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Invitrogen
UBA3 Polyclonal Antibody
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FIGURE: 1 / 3
UBA3 Antibody (PA5-116096) in IHC (P)
Immunohistochemistry analysis of UBA3 in rat testis tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with UBA3 polyclonal antibody (Product # PA5-116096) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-116096
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human UBA3(Accession Q8TBC4), corresponding to amino acid residues N341-N391.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total UBA3.
Target Information
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp566J164; MGC22384; NEDD8-activating enzyme E1 catalytic subunit; NEDD8-activating enzyme E1 subunit 2; NEDD8-activating enzyme E1C; Nedd8-activating enzyme hUba3; similar to yeast UBA3; ubiquitin-like protein, NEDD8, activating enzyme; UBA3, ubiquitin-activating enzyme E1 homolog; UBE1C; Ubiquitin-activating enzyme 3; Ubiquitin-activating enzyme E1C; ubiquitin-activating enzyme E1C (homologous to yeast UBA3); ubiquitin-activating enzyme E1C (UBA3 homolog, yeast); Ubiquitin-like modifier-activating enzyme 3; unnamed protein product
Gene Aliases: A830034N06Rik; AI256736; AI848246; AW546539; hUBA3; NAE2; UBA3; UBE1C
UniProt ID: (Human) Q8TBC4, (Mouse) Q8C878, (Rat) Q99MI7
Entrez Gene ID: (Human) 9039, (Mouse) 22200, (Rat) 117553
nucleotide binding
protein binding
ATP binding
small protein activating enzyme activity
ligase activity
NEDD8 activating enzyme activity
NEDD8 transferase activity
identical protein binding
protein heterodimerization activity
acid-amino acid ligase activity
ligand-dependent nuclear receptor binding
ubiquitin-protein ligase
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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