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Invitrogen
TLE1 Polyclonal Antibody
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FIGURE: 1 / 1
TLE1 Antibody (PA5-120212) in WB
Western blot analysis of TLE1 in extracts of Mouse liver. Samples were incubated with TLE1 Polyclonal antibody (Product # PA5-120212) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-120212
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 256-355 of human TLE1 (NP_005068.2).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: Mouse liver. The target is usually found in the following locations: Nucleus.
Immunogen sequence: DPSSPRASPA HSPRENGIDK NRLLKKDASS SPASTASSAS STSLKSKEMS LHEKASTPVL KSSTPTPRSD MPTPGTSATP GLRPGLGKPP AIDPLVNQAA
Target Information
Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Grg-1; Groucho-related protein 1; RP11-203D19.1; TLE 1; transducin-like enhancer of split 4 (homolog of Drosophila E(spl)) - like; Transducin-like enhancer protein 1
Gene Aliases: C230057C06Rik; Estm14; Grg-1; Grg1; Tle1; Tle4l
UniProt ID: (Mouse) Q62440
Entrez Gene ID: (Mouse) 21885
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
positive regulation of gene expression
Wnt signaling pathway
negative regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of transcription, DNA-templated
negative regulation of anoikis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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