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Invitrogen
HADHA Polyclonal Antibody
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FIGURE: 1 / 12
HADHA Antibody (PA5-53317) in ICC/IF
Immunofluorescence analysis of HADHA was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with HADHA Polyclonal Antibody (Product # PA5-53317) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A3... View More
Product Details
PA5-53317
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human HADHA. Recombinant protein control fragment (Product #RP-91063).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Amount
10 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: IEYLEEVAIT FAKGLADKKI SPKRDKGLVE KLTAYAMTIP FVRQQVYKKV EEKVRKQTKG LYPAPLKIID VVKTGIEQGS DAGYLCESQK FGELVMTKES KALMGLYHGQ VLCKKNKFGA PQKDVKHLAI LGAGLMGAGI AQVSVDK
Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 85%.
Target Information
This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3-ketoacyl-Coenzyme A (CoA) thiolase, alpha subunit; 3-oxoacyl-CoA thiolase; 78 kDa gastrin-binding protein; gastrin-binding protein; hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), alpha subunit; hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit; long-chain 2-enoyl-CoA hydratase; long-chain-3-hydroxyacyl-CoA dehydrogenase; mitochondrial long-chain 2-enoyl-Coenzyme A (CoA) hydratase, alpha subunit; mitochondrial long-chain L-3-hydroxyacyl-Coenzyme A (CoA) dehydrogenase, alpha subunit; mitochondrial trifunctional enzyme, alpha subunit; mitochondrial trifunctional protein, alpha subunit; MLCL AT; Monolysocardiolipin acyltransferase; TP-alpha; Trifunctional enzyme subunit alpha, mitochondrial; unnamed protein product
Gene Aliases: C77020; ECHA; GBP; HADH; HADHA; LCEH; LCHAD; MLCL AT; MTPA; TP-ALPHA
UniProt ID: (Human) P40939, (Mouse) Q8BMS1
Entrez Gene ID: (Human) 3030, (Mouse) 97212
catalytic activity
3-hydroxyacyl-CoA dehydrogenase activity
acetyl-CoA C-acetyltransferase activity
enoyl-CoA hydratase activity
protein binding
oxidoreductase activity
long-chain-3-hydroxyacyl-CoA dehydrogenase activity
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
transferase activity
lyase activity
3-hydroxyacyl-CoA dehydratase activity
long-chain acyl-CoA hydrolase activity
NAD+ binding
fatty-acyl-CoA binding
acetyl-CoA C-acyltransferase activity
long-chain-enoyl-CoA hydratase activity
protein complex binding
NAD binding
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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