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Invitrogen
DNMT3L Polyclonal Antibody
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FIGURE: 1 / 2
DNMT3L Antibody (PA5-76619) in WB
Western blot was performed using Anti-DNMT3L Polyclonal Antibody (Product # PA5-76619) and a 43 kDa band corresponding to DNMT3L was observed across the cell lines and tissues tested. Uncharacterized bands of ~ 110 kDa and ~30 kDa were observed in tissues. Modified whole cell extracts (1% SDS) (30 µg lysate) of BeWo (Lane 1), HeLa (Lane 2), Jurkat (Lane 3), Caco-2 (Lane 4), PC-3 (Lane 5), SK-BR-3 (Lane 6), tissue extracts (30 µg lysate) of Mouse Testis (Lane 7), Rat Testis (Lane 8), Mouse Kidney (Lane 9) and Mouse Liver (Lane 10) were elec... View More
Product Details
PA5-76619
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant full length Human DNMT3L.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and development. Three families of mammalian DNA methyltransferases have been identified: DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells and functions as a maintenance methyltransferase, transferring proper methylation patterns to newly synthesized DNA during replication. DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues. DNMT3A and DNMT3B function as de novo methyltransferases that methylate previously unmethylated regions of DNA. DNMT2 is expressed at low levels in adult somatic tissues and its inactivation affects neither de novo nor maintenance DNA methylation. DNMT3L is a catalytically inactive regulatory factor for the DNMT3A and DNMT3B de novo methyltransferases that is expressed at low levels in embryonic stem cells, testis, ovaries, and thymus. These de novo methyltransferases consist of a heterotetrameric complex containing two molecules of DNMT3L, and either two molecules of DNMT3A or DNMT3B. DNMT3L contains an amino-terminal ATRX-DNMT3-DNMT3L (ADD) domain and a carboxy-terminal methyltransferase-like domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytosine-5-methyltransferase 3-like protein; DNA (cytosine-5)-methyltransferase 3-like; DNA (cytosine-5-)-methyltransferase 3-like; Dnmt 3L; human cytosine-5-methyltransferase 3-like protein; MGC1090
Gene Aliases: D6Ertd14e; DNMT3L; ecat7
UniProt ID: (Human) Q9UJW3, (Mouse) Q9CWR8
Entrez Gene ID: (Human) 29947, (Mouse) 54427
placenta development
methylation-dependent chromatin silencing
male meiosis I
spermatogenesis
post-embryonic development
regulation of gene expression
negative regulation of macromolecule biosynthetic process
cell differentiation
methylation
hypermethylation of CpG island
protection of DNA demethylation of female pronucleus
negative regulation of gene expression, epigenetic
negative regulation of transcription, DNA-templated
stem cell differentiation
chorionic trophoblast cell differentiation
genetic imprinting
negative regulation of methylation-dependent chromatin silencing
transposable element silencing by heterochromatin formation
autosome genomic imprinting
transposable element silencing by piRNA-mediated DNA methylation
in utero embryonic development
positive regulation of catalytic activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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