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Invitrogen
WISP1 Polyclonal Antibody
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FIGURE: 1 / 3
WISP1 Antibody (PA5-80228) in IHC (P)
Immunohistochemistry (Paraffin) analysis of WISP1 in paraffin-embedded section of human testicular germ cell tumor tissue using WISP1 Polyclonal Antibody (Product # PA5-80228). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 2 µg/mL dilut... View More
Product Details
PA5-80228
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human WISP1 (354-367aa ADLESYPDFSEIAN).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
4mg trehalose, 0.9mg NaCl, 0.2mg Na2PO4
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat kidney tissue, mouse kidney tissue. IHC: human colorectal adenocarcinoma tissue, human testicular germ cell tumor tissue.
Target Information
This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Alternative splicing of this gene generates 2 transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CCN family member 4; ELM-1; unnamed protein product; WISP-1; WISP1 3'UTR-associated RNA 1; WISP1 overlapping transcript 1 (non-protein coding); WISP1vx; Wnt-1-induced secreted protein; WNT1 induced secreted protein 1; WNT1 inducible signaling pathway protein 1; WNT1-inducible-signaling pathway protein 1
Gene Aliases: AW146261; CCN4; Elm1; WISP1; WISP1-OT1; WISP1-UT1; WISP1c; WISP1i; WISP1tc
UniProt ID: (Human) O95388, (Rat) Q99PP0, (Mouse) O54775
Entrez Gene ID: (Human) 8840, (Rat) 65154, (Mouse) 22402
osteoblast differentiation
regulation of cytokine production
cell adhesion
signal transduction
cell-cell signaling
positive regulation of smooth muscle cell migration
Wnt signaling pathway
positive regulation of Wnt signaling pathway
osteoclast differentiation
negative regulation of chondrocyte differentiation
glucose homeostasis
regulation of cell differentiation
positive regulation of cell differentiation
negative regulation of fat cell differentiation
positive regulation of osteoblast differentiation
positive regulation of smooth muscle cell proliferation
positive regulation of inflammatory response
regulation of multicellular organismal process
bone development
positive regulation of wound healing
regulation of cell growth
negative regulation of cell death
positive regulation of cell growth involved in cardiac muscle cell development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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