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          • Primary Antibodies ›
          • SHH Antibodies

          Invitrogen

          SHH Polyclonal Antibody

          View all (25) SHH antibodies

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          Cite SHH Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          SHH Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          SHH Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          SHH Antibody (PA5-88595) in ICC/IF

          Immunofluorescence analysis of SHH in HeLa cells. Samples were incubated with SHH Polyclonal antibody (Product # PA5-88595) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          SHH Antibody in Immunocytochemistry (ICC/IF)
          SHH Antibody in Western Blot (WB)
          SHH Polyclonal Antibody

          Product Details

          PA5-88595

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:100
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthetic peptide corresponding to a sequence within amino acids 231-330 of human Sonic Hedgehog (Shh) (NP_0001841)
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.41 mg/mL

          Amount

          41 µg

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2805022

          Product Specific Information

          Positive Samples: A-549; Cellular Location: Cell membrane, Lipid-anchor, extracellular space

          Immunogen sequence: GRLLYSDFLT FLDRDDGAKK VFYVIETREP RERLLLTAAH LLFVAPHNDS ATGEPEASSG SGPPSGGALG PRALFASRVR PGQRVYVVAE RDGDRRLLPA

          Target Information

          Sonic Hedgehog (SHH), which is expressed only during embryogenesis, is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the Sonic Hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: HHG-1; SHH; Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; ShhNC; sonic hedgehog homolog; Sonic hedgehog protein

          View more View less

          Gene Aliases: HHG1; HLP3; HPE3; MCOPCB5; SHH; ShhNC; SMMCI; TPT; TPTPS

          View more View less

          UniProt ID: (Human) Q15465

          View more View less

          Entrez Gene ID: (Human) 6469

          View more View less

          Function(s)
          endopeptidase activity patched binding calcium ion binding protein binding glycosaminoglycan binding peptidase activity zinc ion binding morphogen activity transferase activity hydrolase activity laminin-1 binding metal ion binding cholesterol-protein transferase activity
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter angiogenesis patterning of blood vessels vasculogenesis metanephros development branching involved in ureteric bud morphogenesis cell fate specification neural crest cell migration kidney development hair follicle development vasculature development heart looping positive regulation of neuroblast proliferation positive regulation of mesenchymal cell proliferation osteoblast development lymphoid progenitor cell differentiation determination of left/right asymmetry in lateral mesoderm regulation of transcription, DNA-templated proteolysis protein import into nucleus endocytosis smoothened signaling pathway cell-cell signaling determination of left/right symmetry pattern specification process ectoderm development neuroblast proliferation axon guidance central nervous system development ventral midline development hindgut morphogenesis heart development skeletal muscle tissue development blood coagulation androgen metabolic process cell proliferation positive regulation of cell proliferation embryonic pattern specification tissue development polarity specification of anterior/posterior axis anterior/posterior pattern specification dorsal/ventral pattern formation mesenchymal cell proliferation gene expression regulation of gene expression positive regulation of gene expression negative regulation of gene expression oligodendrocyte development striated muscle tissue development skeletal muscle cell proliferation positive regulation of skeletal muscle cell proliferation myotube differentiation cell migration intein-mediated protein splicing protein autoprocessing spinal cord dorsal/ventral patterning spinal cord motor neuron differentiation thalamus development forebrain regionalization dorsal/ventral neural tube patterning cell proliferation in external granule layer cerebellar granule cell precursor proliferation positive regulation of cerebellar granule cell precursor proliferation telencephalon regionalization regulation of proteolysis positive regulation of Wnt signaling pathway negative regulation of Wnt signaling pathway neuron differentiation respiratory tube development lung development embryonic limb morphogenesis negative regulation of cell migration male genitalia development prostate gland development thyroid gland development forebrain development midbrain development hindbrain development pancreas development hair follicle morphogenesis negative regulation of proteasomal ubiquitin-dependent protein catabolic process T cell differentiation in thymus negative regulation of T cell differentiation in thymus positive regulation of T cell differentiation in thymus positive regulation of immature T cell proliferation in thymus negative regulation of transcription elongation from RNA polymerase II promoter protein localization to nucleus embryonic forelimb morphogenesis embryonic hindlimb morphogenesis tube development trunk neural crest cell migration T cell proliferation regulation of cell proliferation negative regulation of T cell proliferation negative regulation of protein catabolic process positive regulation of protein import into nucleus odontogenesis of dentin-containing tooth odontogenesis regulation of odontogenesis embryonic digit morphogenesis camera-type eye development negative regulation of apoptotic process CD4-positive or CD8-positive, alpha-beta T cell lineage commitment skin development positive thymic T cell selection negative thymic T cell selection intermediate filament organization cell fate commitment myoblast differentiation negative regulation of cell differentiation negative regulation of neuron differentiation positive regulation of smoothened signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter alpha-beta T cell differentiation positive regulation of alpha-beta T cell differentiation negative regulation of alpha-beta T cell differentiation cell development animal organ development thymus development digestive tract morphogenesis embryonic digestive tract morphogenesis embryonic organ development developmental growth embryonic morphogenesis embryonic foregut morphogenesis positive regulation of skeletal muscle tissue development organ formation anatomical structure formation involved in morphogenesis neuron fate commitment embryonic skeletal system development astrocyte differentiation oligodendrocyte differentiation positive regulation of astrocyte differentiation positive regulation of oligodendrocyte differentiation smooth muscle tissue development branching morphogenesis of an epithelial tube inner ear development formation of anatomical boundary stem cell development epithelial cell proliferation positive regulation of epithelial cell proliferation regulation of developmental process striated muscle cell differentiation positive regulation of striated muscle cell differentiation positive regulation of cell division Bergmann glial cell differentiation palate development canonical Wnt signaling pathway limb development limb bud formation regulation of glial cell proliferation lung morphogenesis lung epithelium development trachea development trachea morphogenesis epithelial tube branching involved in lung morphogenesis branching involved in prostate gland morphogenesis branching involved in salivary gland morphogenesis bud outgrowth involved in lung branching right lung development left lung development lung lobe morphogenesis lung-associated mesenchyme development primary prostatic bud elongation prostate epithelial cord elongation salivary gland cavitation epithelial cell proliferation involved in salivary gland morphogenesis epithelial-mesenchymal cell signaling regulation of prostatic bud formation epithelial-mesenchymal signaling involved in prostate gland development epithelial cell proliferation involved in prostate gland development regulation of epithelial cell proliferation involved in prostate gland development positive regulation of epithelial cell proliferation involved in prostate gland development regulation of mesenchymal cell proliferation involved in prostate gland development mesenchymal smoothened signaling pathway involved in prostate gland development artery development mesenchymal cell proliferation involved in lung development somite development positive regulation of sclerotome development cellular response to lithium ion dopaminergic neuron differentiation stem cell proliferation regulation of stem cell proliferation metanephric mesenchymal cell proliferation involved in metanephros development metanephric collecting duct development negative regulation of canonical Wnt signaling pathway establishment of epithelial cell polarity negative regulation of cholesterol efflux mesenchymal cell apoptotic process apoptotic signaling pathway self proteolysis skeletal muscle fiber differentiation regulation of nodal signaling pathway regulation of protein localization to nucleus negative regulation of dopaminergic neuron differentiation tracheoesophageal septum formation negative regulation of ureter smooth muscle cell differentiation positive regulation of ureter smooth muscle cell differentiation negative regulation of kidney smooth muscle cell differentiation positive regulation of kidney smooth muscle cell differentiation positive regulation of mesenchymal cell proliferation involved in ureter development negative regulation of mesenchymal cell apoptotic process
          It has to be done as per old AB suggested Products section.

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