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Human Betacellulin Recombinant Protein, PeproTech®

Human Betacellulin Protein in Functional Assay (Functional)

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Human Betacellulin Protein (100-50-100UG) in Functional

Bioassay analysis of Human Betacellulin Recombinant Protein, PeproTech® (Product # 100-50-1MG).

Product Image Gallery

Product Details

100-50-100UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 11 publications 11 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species

Human

Published species

Human, Mouse

Expression System

E. coli

Amino acid sequence

DGNSTRSPET NGLLCGDPEE NCAATTTQSK RKGHFSRCPK QYKHYCIKGR CRFVVAEQTP SCVCDEGYIG ARCERVDLFY

Molecular weight

9 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

The ED50 was determined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10^7 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino acid residues, which comprises the mature EGF-homologous portion of theBetacellulin protein.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Regulation of CTCF loop formation during pancreatic cell differentiation.

Nature communications

Lyu X,Rowley MJ,Kulik MJ,Dalton S,Corces VG

100-50 was used in In vitro experiments to suggest an important role for CTCF and cohesin in controlling gene expression during cell differentiation.

Mon Oct 09 00:00:00 EDT 2023

Protocol to generate human pluripotent stem cell-derived pancreatic β cells through methionine and zinc deprivation.

STAR protocols

Sim EZ,Enomoto T,Shiraki N,Kume S

100-50 was used in In vitro experiments to describe steps for a short 5-h methionine deprivation pretreatment followed by the application of zinc-deprived media at definitive endoderm differentiation stages to improve differentiation efficiency.

Wed Mar 22 00:00:00 EDT 2023

FALCON systematically interrogates free fatty acid biology and identifies a novel mediator of lipotoxicity.

Cell metabolism

Wieder N,Fried JC,Kim C,Sidhom EH,Brown MR,Marshall JL,Arevalo C,Dvela-Levitt M,Kost-Alimova M,Sieber J,Gabriel KR,Pacheco J,Clish C,Abbasi HS,Singh S,Rutter JC,Therrien M,Yoon H,Lai ZW,Baublis A,Subramanian R,Devkota R,Small J,Sreekanth V,Han M,Lim D,Carpenter AE,Flannick J,Finucane H,Haigis MC,Claussnitzer M,Sheu E,Stevens B,Wagner BK,Choudhary A,Shaw JL,Pablo JL,Greka A

100-50 was used in In vitro experiments to find that c-MAF-inducing protein (CMIP) protects cells from FFA exposure by modulating Akt signaling.

Tue May 02 00:00:00 EDT 2023

Generation of induced pluripotent stem cell-derived beta-cells in blood amino acids-like medium.

Biology open

Ali M,Kato Y,Shiraki N,Kume S

100-50 was used in In vitro experiments to establish a defined culture system to derive SC-β-cells using a blood amino acids-like medium (BALM).

Wed Mar 15 00:00:00 EDT 2023

Differentiating functional human islet-like aggregates from pluripotent stem cells.

STAR protocols

Barsby T,Ibrahim H,Lithovius V,Montaser H,Balboa D,Vähäkangas E,Chandra V,Saarimäki-Vire J,Otonkoski T

100-50 was used in In vitro experiments to present here a robust and reliable protocol by which to differentiate pancreatic islet-like aggregates (SC-islets) from human pluripotent stem cells.

Fri Dec 16 00:00:00 EST 2022

CODEX, a neural network approach to explore signaling dynamics landscapes.

Molecular systems biology

Jacques MA,Dobrzyński M,Gagliardi PA,Sznitman R,Pertz O

Thu Apr 01 00:00:00 EDT 2021

Therapeutically targeting tumor microenvironment-mediated drug resistance in estrogen receptor-positive breast cancer.

The Journal of experimental medicine

Shee K,Yang W,Hinds JW,Hampsch RA,Varn FS,Traphagen NA,Patel K,Cheng C,Jenkins NP,Kettenbach AN,Demidenko E,Owens P,Faber AC,Golub TR,Straussman R,Miller TW

Mon Mar 05 00:00:00 EST 2018

Betacellulin regulates the proliferation and differentiation of retinal progenitor cells in vitro.

Journal of cellular and molecular medicine

Zhang D,Shen B,Zhang Y,Ni N,Wang Y,Fan X,Sun H,Gu P

Mon Jan 01 00:00:00 EST 2018

Image-Based Profiling Can Discriminate the Effects of Inhibitors on Signaling Pathways under Differential Ligand Stimulation.

SLAS discovery : advancing life sciences R & D

Tanabe K,Inagaki A,Henmi Y,Satake M

100-50 was used in Cell Culture to investigate different cellular stimulation effects on efficacy of image-based compound profiling.

Sun Apr 01 00:00:00 EDT 2018

Insulin mutations impair beta-cell development in a patient-derived iPSC model of neonatal diabetes.

eLife

Balboa D,Saarimäki-Vire J,Borshagovski D,Survila M,Lindholm P,Galli E,Eurola S,Ustinov J,Grym H,Huopio H,Partanen J,Wartiovaara K,Otonkoski T

100-50 was used in In vitro experiments to demonstrate that misfolded proinsulin impairs developing beta-cell proliferation without increasing apoptosis.

Fri Nov 09 00:00:00 EST 2018

EGFR Ligands Differentially Stabilize Receptor Dimers to Specify Signaling Kinetics.

Cell

Freed DM,Bessman NJ,Kiyatkin A,Salazar-Cavazos E,Byrne PO,Moore JO,Valley CC,Ferguson KM,Leahy DJ,Lidke DS,Lemmon MA

Thu Oct 19 00:00:00 EDT 2017

Inhibition of epidermal growth factor receptor tyrosine kinase ameliorates collagen-induced arthritis.

Journal of immunology (Baltimore, Md. : 1950)

Swanson CD,Akama-Garren EH,Stein EA,Petralia JD,Ruiz PJ,Edalati A,Lindstrom TM,Robinson WH

Sun Apr 01 00:00:00 EDT 2012

Differentiation of mesenchymal stem cells derived from pancreatic islets and bone marrow into islet-like cell phenotype.

PloS one

Zanini C,Bruno S,Mandili G,Baci D,Cerutti F,Cenacchi G,Izzi L,Camussi G,Forni M

Mon Aug 27 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: OTTHUMP00000160600; OTTHUMP00000219057; Probetacellulin

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Gene Aliases: BTC

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UniProt ID: (Human) P35070

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Entrez Gene ID: (Human) 685

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Human Betacellulin Recombinant Protein, PeproTech®

Human Betacellulin Protein (100-50-100UG) in Functional

Product Details

100-50-100UG

Western Blot (WB)

ELISA (ELISA)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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