SDS-PAGE analysis of Human Fc gamma RIIIA (CD16a) (176 Val) His-tag (Product # A42534). The specification for purity shows: ≥ 95 % (SDS-PAGE), ≥ 95 % (SEC-HPLC).

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Product Details

A42534

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-Dependent

Binding assay (Binding)

Assay-dependent

Product Specifications

Species

Human

Expression System

CHO cells

Amino acid sequence

Human CD16a, amino acids Met1-Gln208 (Accession # P08637-1) with natural variant F176V with a C-terminal His-tag

Tag

His-tag

Molecular weight

23.3 kDa

Class

Recombinant

Type

Protein

Purity

≥95 % as determined by SDS-PAGE. ≥95 % as determined by SEC-HPLC.

Endotoxin concentration

<1.0 EU/µg

Activity

Loaded Recombinant Human CD16a/FCGR3A/Fc gamma RIIA Protein His Tag on His1K Biosensor, can bind Rituximab with an affinity constant of 0.46 µM as determined in BLI assay.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.2, with 5% mannitol, 5% trehalose, 0.01% Tween 80

Contains

no preservative

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient

Product Specific Information

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Target Information

CD16, known as Fc gamma receptor III, exists in two distinct isoforms: Fc gamma RIIIA and Fc gamma RIIIB. These isoforms are encoded by the genes FCGR3A and FCGR3B, respectively, which are located on chromosome 1 and share a high degree of similarity. Fc gamma RIIIA is expressed as a polypeptide-anchored form on monocytes, macrophages, and lymphocytes such as natural killer (NK) cells, but not on T or B cells. This receptor recognizes the Fc portion of immunoglobulin G (IgG) and is involved in the clearance of immune complexes from circulation. Upon binding IgG, Fc gamma RIIIA activates signal transduction pathways that result in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release, and antigen presentation. Fc gamma RIIIB is expressed on polymorphonuclear neutrophils (PMN) as a glycosylphosphatidylinositol (GPI)-anchored form. It plays a role in similar immune functions, including cellular-mediated cytotoxicity and enhancement of virus infections. Mutations in the FCGR3A gene have been associated with Immunodeficiency 20 and linked to susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD16-I; CD16-II; CD16a; CD16a antigen; CD16b; CD32; CD32 receptor 2; CDw32; Fc fragment of IgG low affinity IIIa receptor (CD16a) isoform FCGR3A_1; Fc fragment of IgG low affinity IIIa receptor (CD16a) isoform FCGR3A_2; Fc fragment of IgG receptor IIIa; Fc fragment of IgG receptor IIIb; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III-A; Fc gamma receptor III-B; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RII-b; Fc-gamma RII-c; Fc-gamma RIII; Fc-gamma RIII-alpha; Fc-gamma RIII-beta; fc-gamma RIIIb; Fc-gamma-RIIb; Fc-gamma-RIIc; FCG2; FcgammaRIIIA; FcgammaRIIIb; FCGR2; FCGR2A; FCGR2B; FCGR2C; FcR-10; FcRII-b; FcRII-c; IgG Fc receptor III-1; IgG Fc receptor III-2; IgG Fc receptor III-A; IgG receptor precursor; immunoglobulin G Fc receptor III; low affinity IgG receptor; low affinity IgG receptor CD16; low affinity IgG receptor precursor; Low affinity immunoglobulin gamma Fc region receptor III-A; low affinity immunoglobulin gamma Fc region receptor III-A/III-B recombinant; Low affinity immunoglobulin gamma Fc region receptor III-B; low affinity immunoglobulin gamma receptor III-a Fc fragment; neutrophil-specific antigen NA; RP11-5K23.1; unnamed protein product

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Gene Aliases: CD16; CD16-I; CD16-II; CD16A; CD16B; FCG3; FCGR3; FCGR3A; FCGR3B; FCGRIII; FcGRIIIA; FCR-10; FCRIII; FCRIIIA; FCRIIIb; IGFR3; IMD20

Human Fc gamma RIIIA (CD16a) (176 Val) His-tag Recombinant Protein

Human Fc gamma RIIIA (CD16a) (176 Val) His-tag Protein (A42534)