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Human HGF Protein (PHG0254) in Bioactivity
MKN45 cells were treated with 100 ng/mL human HGF recombinant protein (Product # PHG0254) for 10 minutes. A western blot was performed using anti-phospho-c-Met (Tyr1349) recombinant rabbit polyclonal antibody (Product # 710792) and a 156 kDa band corresponding to phospho-c-Met (Tyr1349) was observed to be upregulated upon treatment. Tubulin was used as a loading control.
Product Details
PHG0254
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Published species
Human
Expression System
Insect cells
Amino acid sequence
Human HGF recombinant protein is a heterodimer consisting of a 463 amino acid alpha chain and a 234 amino acid beta chain
Molecular weight
70 kDa
Class
Recombinant
Type
Protein
Purity
>98% by SDS-PAGE
Endotoxin concentration
<0.1 ng/µg
Activity
ED50 = 1 - 20 ng/mL; determined by the dose-dependent proliferation of monkey 4MBr-5 cells.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Wet or Dry ice (international)
Product Specific Information
Carrier-Free
Source: (BTI-Tn-5B1-4) Hi-5 Insect cells
Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile PBS containing 0.1% human serum albumin. Make any further dilutions of the reconstituted protein in low endotoxin medium or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA.
Storage: Store the lyophilized protein at ≤-20°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.
Target Information
Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Fibroblast derived tumor cytotoxic factor; fibroblast-derived tumor cytotoxic factor; Hepatocyte growth factor; Hepatocyte growth factor (hepapoietin A; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin A; Hepatopoietin-A; HepatopoietinA; Lung fibroblast derived mitogen; lung fibroblast-derived mitogen; Scatter factor; scatter factor); SF
Gene Aliases: DFNB39; F-TCF; HGF; HGFB; HPTA; SF
UniProt ID: (Human) P14210
Entrez Gene ID: (Human) 3082
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