Automated and accelerated method development

Accelerate and automate your method development using Thermo Scientific Vanquish Method Development HPLC and UHPLC systems. These systems can optimize separation conditions by testing different combinations of buffers on up to six HPLC columns. Vanquish Method Development HPLC and UHPLC systems use Thermo Scientific Chromeleon Chromatography Data System (CDS), which features downloadable eWorkflows to simplify automated column and buffer scouting while enabling secure and central data storage. Achieve fully automated method development with ChromSwordAuto Chromeleon Connect and ChromSword AutoRobust Chromeleon Connect. Chromeleon CDS is also compatible with the S-Matrix® Fusion QbD® software package.


Automated column and eluent screening option

Screen up to 13 eluents by equipping your Vanquish Method Development HPLC and UHPLC system with a solvent selection valve for extensive, automated screening. The Thermo Scientific Viper Method Scouting Kit contains all the tubing and near-zero dead volume connections to screen four columns automatically without the need for any user interaction.

Universal and sensitive detection techniques

Detect all non- or semi-volatile analytes using a near-universal Thermo Scientific Charged Aerosol Detector. Additionally, gain more confidence in identification using the Thermo Scientific ISQ EC and EM single quadrupole mass detectors for peak identification and impurity profiling.

Automated, software-assisted method development

Automate, simplify and accelerate method development. The Vanquish Method Development HPLC and UHPLC systems are controlled by Chromeleon CDS and compatible with leading method development software packages, such as ChromSwordAuto Chromeleon Connect or Fusion QbD software.

Secure data storage

The Chromeleon Data Vault ensures secure data storage and accessibility for users within the CDS infrastructure. With Vanquish Method Development HPLC and UHPLC systems, all data generated is safely backed up in Chromeleon CDS Data Vault, enabling easy data exchange throughout the organization.

Optimized for small and large molecules
When built using Vanquish Flex and Vanquish Horizon UHPLC components, these systems are biocompatible, enabling robust performance, even with high-salt gradients, while minimizing unwanted interactions between the analyte and system components. ChromSwordAuto Chromeleon Connect includes dedicated algorithms for more efficient separations of large biomolecules.

Method validation
Automate design and execution of robustness testing using ChromSword AutoRobust Chromeleon Connect and accelerate your method validation using the workflows available in the Chromeleon 7 Extension Pack ICH templates.

What are the stages of HPLC method development?

  1. Method scouting includes screening column and mobile phase conditions to choose the most promising combination for a successful HPLC separation. Traditionally, method scouting requires a significant amount of effort for manual column and mobile phase switching as well as instrument method creation. This process can be automated and streamlined using Viper fingertight fittings and solvent extension kits, and software packages such as ChromSwordAuto Chromeleon Connect and Fusion QbD.
  2. Method optimization, involving iterative testing of various separation conditions of the HPLC method, is performed to achieve the best possible resolution, speed, etc. Operators must run many samples and repeatedly make decisions based on results. This is the most time-consuming stage of method development and requires a great deal of user experience and knowledge. ChromSwordAuto Chromeleon Connect and Fusion QbD software significantly accelerates this step by rapidly analyzing separation data and automatically optimizing the HPLC method.
  3. Robustness testing is required to determine the impact of parameter variation on separation quality and is crucial for method validation. DOE and robustness testing can be automated using ChromSword AutoRobust Chromeleon Connect or Fusion QbD software.
  4. Method validation is the industry-specific process for determining whether a method is fit for purpose. Secure documentation of the method development process is necessary in a variety of industries. Chromeleon CDS ICH validation templates and documentation of all stages of the workflow can be stored in the secure Chromeleon Data Vault to ensure compliance.


System specifications

The table below provides an overview of the system specifications for the Vanquish Method Development HPLC and UHPLC systems for a standard setup. Not all specifications are listed. Your specifications may differ based on the components of your system. Find the specification sheets for the system components in the Component specifications, below.



Vanquish Method Development HPLC and UHPLC Systems

System pressure, maximum

700, 1000 or 1500 bar, depends on system configuration

Flow range

0-5, 0-8 or 0-10 mL/min, depends on pumps selected

Pump options (default delay volume)

Binary (35, 200 or 400 µL)

Quaternary (679 µL)

Dual (679 µL)


Delay volume depends on pump(s) selected


Yes, depends on system configuration

pH range

2-12, chloride concentration up to 1 mol/L

or 1-13 (chloride concentration up to 0.1 mol/L), 

depends on system configuration

System design


Touchscreen user interface display compatible (optional)

Yes, depends on system configuration

Solvent monitoring system compatible (optional)

Yes, depends on system configuration

Customizable gradient delay volume to simplify method transfer

Yes, depends on system configuration

SmartInject technology for maximum injection precision and improved column lifetime


Injection volume range

Standard: 0.01-25 or 0.01-100 µL (Vanquish Flex/Vanquish Horizon)

Optional: Up to 0.01-100 or 0.01-1000 µL (Vanquish Core)

Depends on sampler selected

Sample cooling

Yes, 4-40°C 

Depends on sampler selected

Sample capacity (standard)

Based on standard 12 mm OD vials (≤ 1.5 mL)

4 racks of 54 vials (≤ 1.5mL) each, other rack formats available
+ capacity of 12 x 22.5 mm OD vials (≤ 10 mL) in the carousel

Sampler extension available

Yes, with Vanquish Charger

Column compartment thermostatting

Yes, 5-120°C, depends on column compartment selected

Still air and forced air

Column compartment pre-heater

Active and passive  
Depends on system configuration

Column compartment post-column cooler


Depends on column compartment selected

Detector choices

Vanquish Charged Aerosol Detector

Vanquish Diode array Detector

Vanquish Fluorescence Detector

Vanquish Multiple Wavelength Detector

Vanquish Variable Wavelength Detector

Refractive Index Detector

Mass Spectrometer


Component specifications

Standard Vanquish Method Development HPLC and UHPLC systems are made up of the following components. Download the associated specifications sheet for more detail on each component.



Vanquish Horizon Method Development UHPLC System 

Vanquish Flex Method Development UHPLC System

Vanquish Core Method Development HPLC System


Download Specification Sheet

Vanquish Binary Pump H

 Vanquish Binary Pump F 
Vanquish Quaternary Pump F 

Vanquish Dual Pump F

Vanquish Binary Pump C 
Vanquish Quaternary Pump C 


Download Specification Sheet

Vanquish Split Sampler HT or

Vanquish Dual Split Sampler HT

Vanquish Split Sampler FT

Vanquish Dual Split Sampler FT

Vanquish Split Sampler CT


Vanquish Split Sampler C

Extended sample capacity (optional)

Download Specification Sheet

Vanquish Charger  

Column compartment

Download Specification Sheet

Up to 3 Vanquish Column Compartment H


Up to 3 Vanquish Column Compartment C

Virtually zero dead volume system connections - Method scouting capabilities

Download Specification Sheet

Automated Viper Method Scouting kit for Vanquish Systems (6036.2807)


2 x Valve 6 position 7 port (6036.1570)

Automated Viper Method Scouting kit for Vanquish Systems (6036.2807)


2 x Valve 6 position 7 port (6230.1530)

Virtually zero dead volume system connections - Eluent scouting capabilities

Extension kit for Automated Method Scouting



Visit our HPLC and UHPLC Detectors page


ChromSword is a trademark of VWR International BVBA. S-Matrix and Fusion QbD are trademarks of S-Matrix Corporation.

Contact us to get a quote or discuss an HPLC/UHPLC solution

For Research Use Only. Not for use in diagnostic procedures.