3D image of t cell surrounded by red blood cells

Learn about assays and techniques for detecting and monitoring cell health and function by flow cytometry. Topics include apoptosis, cell counting, proliferation and viability as well as cell cycle, endocytosis, oxidative stress and more.

Flow cytometry assays features

Scientific poster

histogram plot of cell populations with varying hypoxic conditions

Fluorescent hypoxia sensor for flow cytometry (PDF)

This scientific poster features the methods employed to detect hypoxia in living cells using flow cytometry by a novel fluorogenic hypoxia reagent.

Application note

plot of cellevent caspase reagent fluorescence in response to apoptosis inducing drugs

Flow cytometry analysis of dose response for apoptosis induction (PDF)

This application note describes the use of the Invitrogen Attune NxT Flow Cytometer with Autosampler to evaluate the effectiveness of several cancer drugs to induce apoptosis in a dose-dependent manner in Jurkat cells.


Flow cytometry assays subtopics


Flow cytometry assays learning resources

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Type Title Categories
Application note (2017) Flow cytometry analysis of dose response for apoptosis induction  apoptosis, Attune NxT, Autosampler, flow cytometry, cell toxicity, CellEvent Caspase-3/7 Green 
BioProbes articles (Issues 50– present day) BioProbes Journal of Cell Biology Application cell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification
Guided learning T cell stimulation and proliferation eLearning course antibodies, cell proliferation, cell stimulation, flow cytometry, immunoassays, immunology, immunophenotyping, T cells
Molecular Probes Handbook Viability and cytotoxicity assay reagents—Section 15.2 cell health, cell viability and function, fluorescence microscopy/fluorescence imaging, microplate reader
Molecular Probes Handbook Viability and cytotoxicity assay kits for diverse cell types—Section 15.3 cell health, cell viability and function, fluorescence microscopy/fluorescence imaging, microplate reader
Molecular Probes Handbook Assays for apoptosis and autophagy—Section 15.5 apoptosis, autophagy, flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging
Protocols Annexin V staining protocol for flow cytometry (Invitrogen eBioscience reagents) apoptosis, flow cytometry
Protocols BrdU Staining Protocol for Flow Cytometry (Invitrogen eBioscience reagents) cell proliferation, flow cytometry
Protocols CellTrace CFSE cell proliferation Kit protocol cell proliferation, flow cytometry
Protocols CellTrace Far Red cell proliferation Kit protocol cell proliferation, flow cytometry
Protocols CellTrace Violet cell proliferation Kit protocol cell proliferation, flow cytometry
Protocols LIVE/DEAD Fixable Dead cell stains protocol flow cytometry, viability
Protocols LIVE/DEAD Sperm Viability Kit flow cytometry protocol flow cytometry, viability
Protocols LIVE/DEAD Violet Viability/Vitality Kit protocol flow cytometry, viability
Protocols Pharmacological Induction of Apoptosis with Camptothecin (Invitrogen eBioscience reagents) apoptosis, flow cytometry
Protocols Staining cells with eFluor proliferation dyes for flow cytometry (Invitrogen eBioscience reagents) cell proliferation, flow cytometry
Protocols SYTOX Dead Cell Stains protocol cell viability, flow cytometry
Protocols Viability staining protocol for flow cytometry (Invitrogen eBioscience reagents) cell viability, flow cytometry
Scientific poster (2018) Induced pluripotent stem cell sorting, culture and differentiation to desired cell lineage   Attune/Attune NxT, cell counting, cell sorting, Countess, EVOS, fluorescence activated cell sorting, iSort Automated Cell Sorter, imaging microscopy, stem cell research
Scientific poster (2018) Hypoxia measurements in live and fixed cells using fluorescence microscopy, high-content imaging and flow cytometry   Attune/Attune NxT, brightfield microscopy, CellInsight, EVOS, high content analysis (HCA), hypoxia, spheroids
Scientific poster (2018) Prospective cytometric study evaluating the alkaline phosphatase activity as a potential functional biomarker of primitive malignant cells in acute leukemia   antibodies, Attune/Attune NxT, cancer, cell function, flow cytometry, immunophenotyping, stem cell research
Scientific poster (2018) Synergistic effect of curcumin in combination with proapoptotic drugs in human cell models for chronic (CLL) lymphocytic leukaemia   Attune/Attune NxT Flow Cytometer, cell cycle, cancer, drug development, drug research, flow cytometry
Scientific poster (2018) Differently cell doublet profiles: comparison of light scattering with fluorescence measurement techniques   Attune/Attune NxT Flow Cytometer, cell counting, cell cycle, cell viability, flow cytometry, single cell discrimination
Scientific poster (2018) Fluorescent hypoxia sensor for flow cytometry   apoptosis, Attune/Attune NxT, brightfield microscopy, EVOS, flow cytometry, hypoxia, immunology
Scientific Poster (2016) A novel non-cytotoxic fluorescent dye for cell proliferation analysis in flow cytometry   Attune NxT, cell health, cell proliferation, CellTrace, EVOS, flow cytometry, fluorescence microscopy/fluorescence imaging
Scientific Poster (2016) Cell mediated cytotoxicity in “untouched” whole blood   Attune NxT, cell concentration, cell cycle, flow cytometry assays, viability, whole blood
Scientific Poster (2016) No-lyse no-wash new strategy for CD34+ for absolute cell counting without beads   antibodies, Attune NxT, cell counting, compensation, flow cytometry assays , fluorescent dyes
Scientific Poster (2015) A no lyse, no-wash approach to characterizing phagocyte phenotype and function in whole human blood on the Attune NxT Flow Cytometer   Attune NxT, cell cycle, flow cytometer, flow cytometry assays, immunophenotyping, no lyse, phagocytosis, whole blood
Scientific poster (2014) Copper-safe Click-iT Plus EdU proliferation assay: Improved compatibility with simultaneous phycobiliprotein and fluorescent protein detection cell proliferation, Click-iT, flow cytometry, immunophenotyping
Scientific poster (2014) Flow cytometry multiplexing used to analyze metabolic activity and assess pharmaceutical compound toxicity as a high-throughput screening tool Attune NxT, drug discovery, flow cytometer, flow cytometry assay
Scientific Poster (2014) Click-iT EdU Plus for flow cytometry: enhanced compatibility for multiplexing   Attune NxT, cell proliferation, Click-iT, multicolor flow cytometry
Scientific poster (2013) Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health   apoptosis, ArrayScan, caspase substrates, cell health, cells, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, microplate reader, oxidative stress
Scientific Poster (2013) Cells coming to life: Tools for visualizing immune response   endocytosis/phagocytosis, flow cytometry, fluorescence microscopy/fluorescence imaging, live-cell imaging, pH detection, T cell stimulation
Scientific poster (2013) Improved click chemistry demonstrating EdU cell proliferation with GFP-expressing cells and R-PE-based immunophenotyping   Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometry assays, fluorescent dyes, fluorescent proteins, immunophenotyping
Scientific poster (2013) Monitor caspase 3/7 activity without cell fixation: A novel apoptosis reagent   Alexa Fluor, apoptosis, Attune NxT, caspase substrates, cells, fluorescent dyes, multicolor flow cytometry
Scientific poster (2013) Reactive oxygen probes—A broad range of colors with easier labeling and compatibility with fixation: Novel CellROX reagents   cells, fluorescent dyes, multicolor flow cytometry, oxidative stress
Scientific poster (2013) Reactive oxygen probes—A broad range of colors with easier labeling: Novel CellROX reagents   cells, fluorescent dyes, multicolor flow cytometry, oxidative stress, viability
Scientific poster (2011) The next step in the analysis of lymphocyte proliferation   Alexa Fluor, antibodies, Attune NxT, cell proliferation, CellTrace, flow cytometry assays, fluorescent dyes, live-cell imaging, violet laser-excited reagents
Scientific poster (2010) Analysis of proliferation dynamics in primary and immortalized human cells   Alexa Fluor, cancer, cell proliferation, CellTrace, circulatory system, Click-iT, flow cytometry, fluorescent dyes, fluorescent proteins, nucleic acid quantitation
Scientific poster (2010) Dual pulse labeling using EdU and BrdU to monitor alterations in cellular proliferation by fluorescence microscopy and flow cytometry  Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, drug discovery, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2010) Functional characterization of a novel fluorescent dye for proliferation analysis   Alexa Fluor, cell proliferation, cells, CellTrace, flow cytometry, fluorescent dyes, violet laser-excited reagents
Scientific poster (2010) Non-cytotoxic near-IR DNA stain for cell cycle analysis in living cells   cell cycle, flow cytometry, fluorescent dyes, fluorescent proteins, live-cell imaging, nucleic acid labeling, viability
Scientific poster (2010) Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry and imaging   Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF), immunophenotyping, stem cell research, violet laser-excited reagents
Scientific poster (2010) Simultaneous analysis of cell death mechanisms and oxidative stress using Molecular Probes next generation reagents for imaging and flow cytometry   apoptosis, autophagy, caspase substrates, cells, flow cytometry/flow cytometer, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, oxidative stress
CYToMORE lecture video (2016) Microenvironment cytometry 
Presented by Paul Smith, Cardiff University, UK
cancer, cell cycle, flow cytometry, tumor microenvironments, drug discovery
CYToMORE lecture video (2016) Novel reagent solutions for flow cytometry 
Presented by Jolene Bradford, Thermo Fisher Scientific, USA
Attune NxT, flow cytometry assays
CYToMORE lecture video (2016) RNA flow cytometry 
Presented by Paul Wallace, Roswell Park Cancer Institute, USA
flow cytometry, in situ hybridization, RNA
Technology video Click-iT Plus EdU Proliferation Assay 
Measuring DNA synthesis is the most precise way to detect changes in cell proliferation. Image-based proliferation assays generate spatial and temporal results that can not be detected with other methods. This video describes and compares two of the most referenced image-based proliferation technologies.
cell proliferation, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging
Webinar An introduction to flow cytometric analysis, Part 1: Cell proliferation analysis 
In this 2-part series, we will give an overview of tools and techniques using Invitrogen reagents for flow cytometric analysis of cell proliferation, viability, vitality, and apoptosis.
apoptosis, cell cycle, cell proliferation, multicolor flow cytometry, viability
Webinar An introduction to flow cytometric analysis, Part 2: Cell viability and apoptosis analysis 
In this free webinar, we will discuss flow cytometric analysis of apoptosis and identification of dead cells. Changes introduced by apoptosis can be tested with numerous assays measuring membrane structure, mitochondrial function, metabolism, caspase activity, membrane integrity, and DNA fragmentation. We will also discuss dead cell identification using traditional impermeant nucleic acid dyes.
apoptosis, cell cycle, cell proliferation, multicolor flow cytometry, viability
Webinar DNA content cell cycle analysis using flow cytometry 
Find out how careful acquisition and methodical preparation contribute to accurate and consistent DNA analysis. In this webinar we’ll discuss: an overview of the methods and materials for using flow cytometry to determine cell cycle by measuring DNA content, selection of DNA dyes for live cell and fixed cell analysis and tips and tricks for consistent results
cell counting, cell cycle, cell proliferation, flow cytometry
Webinar Flow cytometry in microbiological research 
In recent years the application of flow cytometry in microbiological research has expanded from detection and quantification of organisms to more complex studies including analysis of host-microbe interactions and detailed spatial and temporal analysis of microbial metabolism in different environments. During this webinar we will discuss how the multi-parametric nature of flow cytometry can be applied to microbiology and the advantages of using this application over traditional microbiological methods.
microbiology, multicolor flow cytometry, viability