3D image of t cell surrounded by red blood cells

Learn about assays and techniques for detecting and monitoring cell health and function by flow cytometry. Topics include apoptosis, cell counting, proliferation and viability as well as cell cycle, endocytosis, oxidative stress and more.

Flow cytometry assays features

Scientific poster

histogram plot of cell populations with varying hypoxic conditions

Fluorescent hypoxia sensor for flow cytometry (PDF)

This scientific poster features the methods employed to detect hypoxia in living cells using flow cytometry by a novel fluorogenic hypoxia reagent.

Application note

plot of cellevent caspase reagent fluorescence in response to apoptosis inducing drugs

Flow cytometry analysis of dose response for apoptosis induction (PDF)

This application note describes the use of the Invitrogen Attune NxT Flow Cytometer with Autosampler to evaluate the effectiveness of several cancer drugs to induce apoptosis in a dose-dependent manner in Jurkat cells.


Flow cytometry assays subtopics


Flow cytometry assays learning resources

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Type Title Categories
Application note (2017) Flow cytometry analysis of dose response for apoptosis induction apoptosis, Attune NxT, Autosampler, flow cytometry, cell toxicity, CellEvent Caspase-3/7 Green 
BioProbes article (2018)A comprehensive resource for state-of-the-art flow cytometry methods. Guidelines for the use of flow cytometry and cell sorting in immunological studiesantibodies and proteins, cell counting, cell cycle, cell proliferation, flow cytometer calibration, flow cytometry, flow cytometry compensation, flow cytometry sample preparation, immunology, immunophenotyping, viability
BioProbes article (2017)An evaluation of dyes for tracking cell proliferation by flow and image cytometrycell proliferation, cell tracking, flow cytometry, fluorescence microscopy/fluorescence imaging
BioProbes article (2017)Journal club: measurement and characterization of apoptosis by flow cytometryapoptosis, caspase substrates, flow cytometry, fluorescence, viability
BioProbes article (2017)Powering up drug discovery—Cancer biology applications using the Attune NxT Flow Cytometerapoptosis, Attune NxT, cancer, flow cytometer, fluorescence, multicolor flow cytometry, viability
BioProbes article (2016)A guide to studying cell proliferation by generational tracing—Tracking cell division with flow cytometryCellTrace, cell cycle, cell proliferation, cell tracking, flow cytometry
BioProbes article (2016)High-speed cell counting with the Attune NxT Flow Cytometer—Accurately measure cell concentrations by flow cytometryAttune NxT, bacteria, cell concentration, cell counting, flow cytometer, immunophenotyping, microbiology, viability
BioProbes article (2015)Journal Club—Multiparameter detection of early apoptosis markers without compensationapoptosis, Attune NxT, cell structure-mitochondria, flow cytometry compensation, fluorescence, immunophenotyping
BioProbes article (2014)Click-iT EdU Flow Cytometry Kits— Simplifying cell proliferation analysiscell proliferation, Click-iT, flow cytometry, fluorescence
BioProbes article (2014)Multiplex your cell proliferation assay with GFP, RFP, and R-PE probes—Click-iT Plus EdU Proliferation Kits for imaging and flow cytometrycell proliferation, Click-iT, cell proliferation, multicolor flow cytometry
BioProbes article (2014)Tracking cells through time and space—Choose from fluorescent Dyes, Qdot nanocrystals, and targeted fluorescent proteinsCellTrace, cell tracking, fluorescence microscopy/fluorescence imaging, fluorescent proteins,  live cells
BioProbes article (2013)Checking vital signs: Don't let dead cells mislead you—Molecular Probes cell health assays for flow cytometryapoptosis, caspase substrate, cell cycle, cell proliferation, cell tracking, Click-iT, flow cytometry, fluorescence, immunophenotyping, oxidative stress, viability,
BioProbes article (2011)Multiplex your cell proliferation assay with GFP, RFP, and R-PE probes—Click-iT Plus EdU Proliferation Kits for imaging and flow cytometrycell proliferation, Click-iT, cell proliferation, multicolor flow cytometry
BioProbes article (2011)Superior live-cell tracing, generation after generation—The CellTrace Far Red Cell Proliferation Kitcell proliferation, CellTrace, flow cytometry, fluorescence
BioProbes article (2010)A ratiometric approach to apoptosis detection by flow cytometryapoptosis, flow cytometry, fluorescence
BioProbes article (2010)Advanced cell proliferation analysisAttune NxT cell proliferation, cell tracking, flow cytometry, fluorescence
BioProbes article (2009) A growing list of tools for the violet laser Alexa Fluor, apoptosis, cell cycle, Click-iT, flow cytometry, fluorescent dyes, immunophenotyping, nanocrystals, Qdot, viability
BioProbes article (2009) Beyond classification—Click-iT EDU outperforms BRDU across all species Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, nucleic acid detection, nucleic acid quantitation, nucleosides, sample preparation
BioProbes article (2009) Dead cell stains in flow cytometry: A comprehensive analysis apoptosis, fluorescent dyes, viability
BioProbes article (2009) Near-infrared dye for live cell cycle analysis cell cycle, flow cytometry, fluorescent dyes, live-cell imaging, nucleic acid detection
BioProbes article (2008) The picture of cell health Alexa Fluor, cell health, cell proliferation, Click-iT, fluorescent dyes, flow cytometry, fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader, nucleic acid quantitation, nucleosides, reagents
BioProbes article (2007) Breakthrough cell proliferation assay for flow cytometry Alexa Fluor, cell proliferation, Click-iT, flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, nucleic acid quantitation
BioProbes article (2007) Dead-cell discrimination in a different light flow cytometry, fluorescent dyes, viability
BioProbes article (2007) Improved results are just a click away Alexa Fluor, cell proliferation, Click-iT, flow cytometry, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, in vivo imaging, multicolor flow cytometry, nucleic acid quantitation, nucleosides
BioProbes article (2007) Investigating apoptosis-induced loss of SYTO dye fluorescence apoptosis, cell structure-mitochondria, enzyme activity detection, flow cytometry, fluorescent dyes
BioProbes article (2007) Looking for apoptosis? antibodies, apoptosis, colorimetric, ELISA, flow cytometry, fluorescence, fluorescent dyes, immunocytochemistry (ICC), protein detection, western blotting
BioProbes article (2007) Multiparametric approaches for studying apoptosis Alexa Fluor, antibodies, apoptosis, cell structure-mitochondria, cell structure-plasma membrane, enzyme activity detection, flow cytometry, fluorescent dyes, viability
BioProbes article (2007) New applications for Vybrant DyeCycle stains Alexa Fluor, cell cycle, cell isolation, drug discovery, flow cytometry, fluorescent dyes, high content analysis, live-cell imaging, multicolor flow cytometry, stem cell research
BioProbes article (2006) Analyzing cell culture health and concentration apoptosis, cell counting, fluorescent dyes, flow cytometry, fluorescence microscopy/fluorescence imaging, microplate reader, particles, viability
BioProbes article (2006)Analyzing cell culture health and concentration apoptosis, cell counting, fluorescent dyes, flow cytometry, fluorescence microscopy/fluorescence imaging, microplate reader, particles, viability
Guided learningT cell stimulation and proliferation eLearning courseantibodies, cell proliferation, cell stimulation, flow cytometry, immunoassays, immunology, immunophenotyping, T cells
Molecular Probes HandbookViability and cytotoxicity assay reagents—Section 15.2cell health, cell viability and function, fluorescence microscopy/fluorescence imaging, microplate reader
Molecular Probes HandbookViability and cytotoxicity assay kits for diverse cell types—Section 15.3cell health, cell viability and function, fluorescence microscopy/fluorescence imaging, microplate reader
Molecular Probes HandbookAssays for apoptosis and autophagy—Section 15.5apoptosis, autophagy, flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging
ProtocolsAnnexin V staining protocol for flow cytometry (Invitrogen eBioscience reagents)apoptosis, flow cytometry
ProtocolsBrdU Staining Protocol for Flow Cytometry (Invitrogen eBioscience reagents)cell proliferation, flow cytometry
ProtocolsCellTrace CFSE cell proliferation Kit protocolcell proliferation, flow cytometry
ProtocolsCellTrace Far Red cell proliferation Kit protocolcell proliferation, flow cytometry
ProtocolsCellTrace Violet cell proliferation Kit protocolcell proliferation, flow cytometry
ProtocolsLIVE/DEAD Fixable Dead cell stains protocolflow cytometry, viability
ProtocolsLIVE/DEAD Sperm Viability Kit flow cytometry protocolflow cytometry, viability
ProtocolsLIVE/DEAD Violet Viability/Vitality Kit protocolflow cytometry, viability
ProtocolsPharmacological Induction of Apoptosis with Camptothecin (Invitrogen eBioscience reagents)apoptosis, flow cytometry
ProtocolsStaining cells with eFluor proliferation dyes for flow cytometry (Invitrogen eBioscience reagents)cell proliferation, flow cytometry
ProtocolsSYTOX Dead Cell Stains protocolcell viability, flow cytometry
ProtocolsViability staining protocol for flow cytometry (Invitrogen eBioscience reagents)cell viability, flow cytometry
Scientific poster (2018) Induced pluripotent stem cell sorting, culture and differentiation to desired cell lineage Attune/Attune NxT, cell counting, cell sorting, Countess, EVOS, fluorescence activated cell sorting, iSort Automated Cell Sorter, imaging microscopy, stem cell research
Scientific poster (2018) Hypoxia measurements in live and fixed cells using fluorescence microscopy, high-content imaging and flow cytometry Attune/Attune NxT, brightfield microscopy, CellInsight, EVOS, high content analysis (HCA), hypoxia, spheroids
Scientific poster (2018) Prospective cytometric study evaluating the alkaline phosphatase activity as a potential functional biomarker of primitive malignant cells in acute leukemia antibodies, Attune/Attune NxT, cancer, cell function, flow cytometry, immunophenotyping, stem cell research
Scientific poster (2018) Synergistic effect of curcumin in combination with proapoptotic drugs in human cell models for chronic (CLL) lymphocytic leukaemia Attune/Attune NxT Flow Cytometer, cell cycle, cancer, drug development, drug research, flow cytometry
Scientific poster (2018) Differently cell doublet profiles: comparison of light scattering with fluorescence measurement techniques Attune/Attune NxT Flow Cytometer, cell counting, cell cycle, cell viability, flow cytometry, single cell discrimination
Scientific poster (2018) Fluorescent hypoxia sensor for flow cytometry apoptosis, Attune/Attune NxT, brightfield microscopy, EVOS, flow cytometry, hypoxia, immunology
Scientific Poster (2016) A novel non-cytotoxic fluorescent dye for cell proliferation analysis in flow cytometry Attune NxT, cell health, cell proliferation, CellTrace, EVOS, flow cytometry, fluorescence microscopy/fluorescence imaging
Scientific Poster (2016) Cell mediated cytotoxicity in “untouched” whole blood Attune NxT, cell concentration, cell cycle, flow cytometry assays, viability, whole blood
Scientific Poster (2016) No-lyse no-wash new strategy for CD34+ for absolute cell counting without beads antibodies, Attune NxT, cell counting, compensation, flow cytometry assays , fluorescent dyes
Scientific Poster (2015) A no lyse, no-wash approach to characterizing phagocyte phenotype and function in whole human blood on the Attune NxT Flow Cytometer Attune NxT, cell cycle, flow cytometer, flow cytometry assays, immunophenotyping, no lyse, phagocytosis, whole blood
Scientific poster (2014)Copper-safe Click-iT Plus EdU proliferation assay: Improved compatibility with simultaneous phycobiliprotein and fluorescent protein detectioncell proliferation, Click-iT, flow cytometry, immunophenotyping
Scientific poster (2014)Flow cytometry multiplexing used to analyze metabolic activity and assess pharmaceutical compound toxicity as a high-throughput screening toolAttune NxT, drug discovery, flow cytometer, flow cytometry assay
Scientific Poster (2014) Click-iT EdU Plus for flow cytometry: enhanced compatibility for multiplexing Attune NxT, cell proliferation, Click-iT, multicolor flow cytometry
Scientific poster (2013) Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health apoptosis, ArrayScan, caspase substrates, cell health, cells, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, microplate reader, oxidative stress
Scientific Poster (2013) Cells coming to life: Tools for visualizing immune response endocytosis/phagocytosis, flow cytometry, fluorescence microscopy/fluorescence imaging, live-cell imaging, pH detection, T cell stimulation
Scientific poster (2013) Improved click chemistry demonstrating EdU cell proliferation with GFP-expressing cells and R-PE-based immunophenotyping Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometry assays, fluorescent dyes, fluorescent proteins, immunophenotyping
Scientific poster (2013) Monitor caspase 3/7 activity without cell fixation: A novel apoptosis reagentAlexa Fluor, apoptosis, Attune NxT, caspase substrates, cells, fluorescent dyes, multicolor flow cytometry
Scientific poster (2013) Reactive oxygen probes—A broad range of colors with easier labeling and compatibility with fixation: Novel CellROX reagentscells, fluorescent dyes, multicolor flow cytometry, oxidative stress
Scientific poster (2013) Reactive oxygen probes—A broad range of colors with easier labeling: Novel CellROX reagentscells, fluorescent dyes, multicolor flow cytometry, oxidative stress, viability
Scientific poster (2011) The next step in the analysis of lymphocyte proliferationAlexa Fluor, antibodies, Attune NxT, cell proliferation, CellTrace, flow cytometry assays, fluorescent dyes, live-cell imaging, violet laser-excited reagents
Scientific poster (2010) Analysis of proliferation dynamics in primary and immortalized human cells Alexa Fluor, cancer, cell proliferation, CellTrace, circulatory system, Click-iT, flow cytometry, fluorescent dyes, fluorescent proteins, nucleic acid quantitation
Scientific poster (2010) Dual pulse labeling using EdU and BrdU to monitor alterations in cellular proliferation by fluorescence microscopy and flow cytometry Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, drug discovery, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2010) Functional characterization of a novel fluorescent dye for proliferation analysis Alexa Fluor, cell proliferation, cells, CellTrace, flow cytometry, fluorescent dyes, violet laser-excited reagents
Scientific poster (2010) Non-cytotoxic near-IR DNA stain for cell cycle analysis in living cells cell cycle, flow cytometry, fluorescent dyes, fluorescent proteins, live-cell imaging, nucleic acid labeling, viability
Scientific poster (2010) Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry and imagingAlexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF), immunophenotyping, stem cell research, violet laser-excited reagents
Scientific poster (2010) Simultaneous analysis of cell death mechanisms and oxidative stress using Molecular Probes next generation reagents for imaging and flow cytometry apoptosis, autophagy, caspase substrates, cells, flow cytometry/flow cytometer, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, oxidative stress
CYToMORE lecture video (2016) Microenvironment cytometry 
Presented by Paul Smith, Cardiff University, UK
cancer, cell cycle, flow cytometry, tumor microenvironments, drug discovery
CYToMORE lecture video (2016) Novel reagent solutions for flow cytometry 
Presented by Jolene Bradford, Thermo Fisher Scientific, USA
Attune NxT, flow cytometry assays
CYToMORE lecture video (2016) RNA flow cytometry 
Presented by Paul Wallace, Roswell Park Cancer Institute, USA
flow cytometry, in situ hybridization, RNA
Technology videoClick-iT Plus EdU Proliferation Assay 
Measuring DNA synthesis is the most precise way to detect changes in cell proliferation. Image-based proliferation assays generate spatial and temporal results that can not be detected with other methods. This video describes and compares two of the most referenced image-based proliferation technologies.
cell proliferation, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging
WebinarAn introduction to flow cytometric analysis, Part 1: Cell proliferation analysis 
In this 2-part series, we will give an overview of tools and techniques using Invitrogen reagents for flow cytometric analysis of cell proliferation, viability, vitality, and apoptosis.
apoptosis, cell cycle, cell proliferation, multicolor flow cytometry, viability
WebinarAn introduction to flow cytometric analysis, Part 2: Cell viability and apoptosis analysis 
In this free webinar, we will discuss flow cytometric analysis of apoptosis and identification of dead cells. Changes introduced by apoptosis can be tested with numerous assays measuring membrane structure, mitochondrial function, metabolism, caspase activity, membrane integrity, and DNA fragmentation. We will also discuss dead cell identification using traditional impermeant nucleic acid dyes.
apoptosis, cell cycle, cell proliferation, multicolor flow cytometry, viability
WebinarDNA content cell cycle analysis using flow cytometry 
Find out how careful acquisition and methodical preparation contribute to accurate and consistent DNA analysis. In this webinar we’ll discuss: an overview of the methods and materials for using flow cytometry to determine cell cycle by measuring DNA content, selection of DNA dyes for live cell and fixed cell analysis and tips and tricks for consistent results
cell counting, cell cycle, cell proliferation, flow cytometry
WebinarFlow cytometry in microbiological research 
In recent years the application of flow cytometry in microbiological research has expanded from detection and quantification of organisms to more complex studies including analysis of host-microbe interactions and detailed spatial and temporal analysis of microbial metabolism in different environments. During this webinar we will discuss how the multi-parametric nature of flow cytometry can be applied to microbiology and the advantages of using this application over traditional microbiological methods.
microbiology, multicolor flow cytometry, viability