Gene Expression Analysis
Comprehensive solutions for gene expression profiling, validation, and screening
Gene expression profiling simultaneously compares the expression levels of multiple genes between two or more samples. This analysis can help scientists identify the molecular basis of phenotypic differences and select gene expression targets for in-depth study. Gene expression profiling provides valuable insight into the role of differential gene expression in normal biological and disease processes.
Comprehensive solutions for gene expression profiling, validation, and screening are supported by sequencing, microarrays, and real-time PCR technologies. For discovery, in particular de novo discovery, of differentially expressed genes (DEGs), transcriptome sequencing provides a hypothesis-neutral approach that allows interrogation of any gene in the sample, with a wide dynamic range to allow differential gene expression analysis of low-expressing genes. For analysis deep within the known transcriptome, transcriptome-level microarrays provide fast profiling of coding and non-coding genes, exons, and transcript isoforms. Well-annotated gene expression analysis can be performed with gene-level microarrays. DEGs can be validated using mid-throughput real-time PCR formats such as OpenArray™ and TaqMan™ Array Cards, and the resulting genes of interest can be quantified more precisely by digital PCR or real-time PCR with TaqMan™ Gene Expression Assays. Screening experiments can also be performed to further examine the behavior of these genes across a larger population of samples.
Transcriptome sequencing by next-generation sequencing enables the discovery of differentially expressed genes using a hypothesis-neutral approach. Sanger sequencing methods can also be used for gene expression profiling and analysis.
Real-time PCR is the gold-standard technique for validation of differential gene expression profiles. Our comprehensive offering of TaqMan Gene Expression Assays and flexible formats enable fast and cost-effective results.
Digital PCR enables highly precise measurements and is able to resolve gene expression level changes of two-fold or less. Digital PCR can also be used to determine the absolute quantification of a transcript without the need for a reference gene.
Designed with up-to-date content from public sequence data sources, our microarrays enable fast, detailed transcriptome-wide expression profiling, even from challenging and precious samples.
For Research Use Only. Not for use in diagnostic procedures.