Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
The QuantStudio™ 12K Flex Real-Time PCR System helps streamline targeted discovery and validation, pathogen detection, and biomarker identification and screening, at a scale previously unattainable. Achieve the highest sample throughput for mid-density gene expression analysis - in a single day, one user can generate >42,000 gene expression data points without the use of robotics.
Isolate and purify total RNA.
![]() | RNA extraction for Real-Time PCR |
Select inventoried TaqMan® Assays and configure the OpenArray® plate layout using the downloadable workbook tool. QuantStudio™ TaqMan® OpenArray® Real-Time PCR Plates are delivered with assays dried down in the through-holes you specify. Mix DNA samples with master mix in OpenArray® 384-well sample plate.
![]() | TaqMan® OpenArray® Real-Time PCR Plates Configure a Gene Expression OpenArray® Plate using pre-designed TaqMan® Gene Expression Assays. OpenArray® Master Mix, Loader Tips & other plastics: |
Reverse transcription: Choosing the correct reverse transcriptase (RT) for cDNA synthesis is critical to obtaining high yields of cDNA to improve the range of your real-time RT-PCR assays. The SuperScript® VILO™ cDNA Synthesis Kit is designed to increase cDNA yields and improve the range of your qRT-PCR assays. You obtain the same relative representation in your cDNA, irrespective regardless of the abundance of your gene of interest.
![]() | SuperScript® VILO™ cDNA Synthesis Kit – 50 reactions |
Pre-amplification: Pre-amplification is an optional and intermediate amplification step that makes it possible to perform gene expression analysis starting with a limited quantity of RNA. Pre-amplification enables you to stretch your precious sample into many more real-time PCR reactions.
Prepare your sample mix: Mix cDNA or the product of your pre-amplification step with TaqMan® OpenArray® Genotyping Master Mix in a TaqMan® OpenArray® 384-well sample plate.
Load your sample mixes onto the TaqMan® OpenArray® Real-Time PCR Plate using the OpenArray® AccuFill™ System. Next, insert the TaqMan® OpenArray® Real-Time PCR Plate into the OpenArray® case filled with immersion fluid, and seal with adhesive. Run the reactions and collect your data using the OpenArray® Real-Time PCR Instrument.
![]() | TaqMan® OpenArray® Real-Time PCR Master Mix |
Load sample mixes onto TaqMan® OpenArray® Real-Time PCR Plate using the QuantStudio 12K Flex OpenArray® AccuFill™ System. The plate is encased with an alloy bottom for easy handling. Simply apply the adhesive lid and fill with immersion fluid. Perform a real-time plate read using the QuantStudio™ 12K Flex system.
![]() | QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block |
Acquire, analyze, and manage data quickly and easily. Amplification curves are presented for review, and data is easily manipulated to calculate Ct and Tm, as well as ΔCt and ΔΔCt measurements.
![]() | ExpressionSuite Software |
Isolate and purify total RNA.
![]() | RNA extraction for Real-Time PCR |
Select inventoried TaqMan® Assays and configure the OpenArray® plate layout using the downloadable workbook tool. QuantStudio™ TaqMan® OpenArray® Real-Time PCR Plates are delivered with assays dried down in the through-holes you specify. Mix DNA samples with master mix in OpenArray® 384-well sample plate.
![]() | TaqMan® OpenArray® Real-Time PCR Plates Configure a Gene Expression OpenArray® Plate using pre-designed TaqMan® Gene Expression Assays. OpenArray® Master Mix, Loader Tips & other plastics: |
Reverse transcription: Choosing the correct reverse transcriptase (RT) for cDNA synthesis is critical to obtaining high yields of cDNA to improve the range of your real-time RT-PCR assays. The SuperScript® VILO™ cDNA Synthesis Kit is designed to increase cDNA yields and improve the range of your qRT-PCR assays. You obtain the same relative representation in your cDNA, irrespective regardless of the abundance of your gene of interest.
![]() | SuperScript® VILO™ cDNA Synthesis Kit – 50 reactions |
Pre-amplification: Pre-amplification is an optional and intermediate amplification step that makes it possible to perform gene expression analysis starting with a limited quantity of RNA. Pre-amplification enables you to stretch your precious sample into many more real-time PCR reactions.
Prepare your sample mix: Mix cDNA or the product of your pre-amplification step with TaqMan® OpenArray® Genotyping Master Mix in a TaqMan® OpenArray® 384-well sample plate.
Load your sample mixes onto the TaqMan® OpenArray® Real-Time PCR Plate using the OpenArray® AccuFill™ System. Next, insert the TaqMan® OpenArray® Real-Time PCR Plate into the OpenArray® case filled with immersion fluid, and seal with adhesive. Run the reactions and collect your data using the OpenArray® Real-Time PCR Instrument.
![]() | TaqMan® OpenArray® Real-Time PCR Master Mix |
Load sample mixes onto TaqMan® OpenArray® Real-Time PCR Plate using the QuantStudio 12K Flex OpenArray® AccuFill™ System. The plate is encased with an alloy bottom for easy handling. Simply apply the adhesive lid and fill with immersion fluid. Perform a real-time plate read using the QuantStudio™ 12K Flex system.
![]() | QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block |
Acquire, analyze, and manage data quickly and easily. Amplification curves are presented for review, and data is easily manipulated to calculate Ct and Tm, as well as ΔCt and ΔΔCt measurements.
![]() | ExpressionSuite Software |
For Research Use Only. Not for human or animal therapeutic or diagnostic use.