Which RNA Isolation Kit is Right for Your qRT-PCR Experiment?

 Order nowOrder nowOrder nowOrder now
 Gold standard for highly pure, intact RNAHigh-quality RNA in less than 20 minutesHigh-throughput purification of RNA and DNAComplete, no purification system for qRT-PCR results
  Trizol Reagents PureLink Kits MagMAX Kits Cells to Ct Kits
RecommendationsMost Cited, Top Seller  Best for qRT-PCR
Isolation methodOrganic reagentSilica filter, column format (convenience)Magnetic beads (scalability, flexibility)Chemical lysis (time to qRT-PCR results)
High throughput compatibleNoYes (plate format available)YesYes
RT reagents includedNoNoNoYes
qPCR reagents includedNoNoNoYes
Prep time~1hr< 20 minutes~45 minutes10 minutes
Compatible sample typesMost sample types, particularly more difficult to lyseCells and tissuesCells, blood, plantsCells
Amount of starting material100 mg of tissue or 107 cells (requires 1ml reagent)Up to 200 mg tissue, 5x106 to 1 x 108 cellsUp to 100 mg tissue, Up to 5x106 cells1–10,000 cells

Which RNA Isolation Kit is Right for Your qRT-PCR Experiment?

 Order nowOrder nowOrder nowOrder now
 Gold standard for highly pure, intact RNAHigh-quality RNA in less than 20 minutesHigh-throughput purification of RNA and DNAComplete, no purification system for qRT-PCR results
  Trizol® Reagents PureLink™ Kits MagMAX™ Kits Cells to Ct™ Kits
RecommendationsMost Cited, Top Seller  Best for qRT-PCR
Isolation methodOrganic reagentSilica filter, column format (convenience)Magnetic beads (scalability, flexibility)Chemical lysis (time to qRT-PCR results)
High throughput compatibleNoYes (plate format available)YesYes
RT reagents includedNoNoNoYes
qPCR reagents includedNoNoNoYes
Prep time~1hr< 20 minutes~45 minutes10 minutes
Compatible sample typesMost sample types, particularly more difficult to lyseCells and tissuesCells, blood, plantsCells
Amount of starting material100 mg of tissue or 107 cells (requires 1ml reagent)Up to 200 mg tissue, 5x106 to 1 x 108 cellsUp to 100 mg tissue, Up to 5x106 cells1–10,000 cells
Price per prep range$1.40 - $7.42$4.50 - $6.03$3.00 - $8.00$9.62 - $19.00

Eliminate Genomic DNA Contamination

Genomic DNA contamination can lead to false positive RT-PCR results. We offer a variety of Ambion tools for minimizing genomic DNA contamination from RNA samples prior to RT-PCR. For example, DNA-free DNase Treatment and Removal Reagents are designed for removing contaminating DNA from RNA samples and for the removal of DNase after treatment without Proteinase K treatment and organic extraction. In addition, we offer TURBO DNase enzyme kits, a hyperactive enzyme engineered from wild-type bovine DNase. The proficiency of TURBO DNase enzyme in binding very low concentrations of DNA means that the enzyme is particularly effective in removing trace quantities of DNA contamination.  To prevent cross contamination during PCR experiments, we also offer DNAZap DNA Degradation Solution and RNase-free barrier pipette tips.

Less time to real-time for your RNA applications