RNA Extraction for Real-Time PCR

Which RNA Isolation Kit is Right for Your qRT-PCR Experiment?

	Gold standard for highly pure, intact RNA	High-quality RNA in less than 20 minutes	High-throughput purification of RNA and DNA	Complete, no purification system for qRT-PCR results
	Order now	Order now	Order now	Order now
	Trizol Reagents	PureLink Kits	MagMAX Kits	Cells to Ct Kits
Recommendations	Most Cited, Top Seller			Best for qRT-PCR
Isolation method	Organic reagent	Silica filter, column format (convenience)	Magnetic beads (scalability, flexibility)	Chemical lysis (time to qRT-PCR results)
High throughput compatible	No	Yes (plate format available)	Yes	Yes
RT reagents included	No	No	No	Yes
qPCR reagents included	No	No	No	Yes
Prep time	~1hr	< 20 minutes	~45 minutes	10 minutes
Compatible sample types	Most sample types, particularly more difficult to lyse	Cells and tissues	Cells, blood, plants	Cells
Amount of starting material	100 mg of tissue or 10⁷ cells (requires 1ml reagent)	Up to 200 mg tissue, 5x10⁶to 1 x 10⁸cells	Up to 100 mg tissue, Up to 5x10⁶ cells	1–10,000 cells

Which RNA Isolation Kit is Right for Your qRT-PCR Experiment?

	Gold standard for highly pure, intact RNA	High-quality RNA in less than 20 minutes	High-throughput purification of RNA and DNA	Complete, no purification system for qRT-PCR results
	Order now	Order now	Order now	Order now
	Trizol® Reagents	PureLink™ Kits	MagMAX™ Kits	Cells to Ct™ Kits
Recommendations	Most Cited, Top Seller			Best for qRT-PCR
Isolation method	Organic reagent	Silica filter, column format (convenience)	Magnetic beads (scalability, flexibility)	Chemical lysis (time to qRT-PCR results)
High throughput compatible	No	Yes (plate format available)	Yes	Yes
RT reagents included	No	No	No	Yes
qPCR reagents included	No	No	No	Yes
Prep time	~1hr	< 20 minutes	~45 minutes	10 minutes
Compatible sample types	Most sample types, particularly more difficult to lyse	Cells and tissues	Cells, blood, plants	Cells
Amount of starting material	100 mg of tissue or 10⁷ cells (requires 1ml reagent)	Up to 200 mg tissue, 5x10⁶to 1 x 10⁸cells	Up to 100 mg tissue, Up to 5x10⁶ cells	1–10,000 cells
Price per prep range	$1.40 - $7.42	$4.50 - $6.03	$3.00 - $8.00	$9.62 - $19.00

Eliminate Genomic DNA Contamination

Genomic DNA contamination can lead to false positive RT-PCR results. We offer a variety of Ambion tools for minimizing genomic DNA contamination from RNA samples prior to RT-PCR. For example, DNA-free DNase Treatment and Removal Reagents are designed for removing contaminating DNA from RNA samples and for the removal of DNase after treatment without Proteinase K treatment and organic extraction. In addition, we offer TURBO DNase enzyme kits, a hyperactive enzyme engineered from wild-type bovine DNase. The proficiency of TURBO DNase enzyme in binding very low concentrations of DNA means that the enzyme is particularly effective in removing trace quantities of DNA contamination. To prevent cross contamination during PCR experiments, we also offer DNAZap DNA Degradation Solution and RNase-free barrier pipette tips.