RNA Structure/Function Studies

Tools for RNA structure, function, and research

RNA structure is thought to play a central role in many cellular processes, including transcription initiation, elongation and termination, mRNA splicing, and retroviral infection of eukaryotic cells.  Elucidating the mechanistic aspects of these intricate processes will require detailed understanding of the underlying RNA structure. The structure of RNA molecules are typically comprised of single-stranded and double-stranded regions that give rise to complex three-dimensional structures. These structures are involved in the molecule's interactions with other nucleic acids, proteins, and small molecules.  We have developed an extensive portfolio of products for the synthesis and modification of RNA in order to further understand the role of RNA structure.

Enzymes for researching RNA structure and function
  • RNA-Grade Ribonucleases: A collection of ribonucleases (RNases A and T1) that are optimized for researchers performing RNA structure, RNA sequencing, protein footprinting and boundary experiments.
  • Poly(A) Polymerase: Catalyzes the addition of adenosine to the 3´ end of RNA in a sequence-independent fashion.
  • T7 RNA Polymerase: Catalyzes the 5´→3´ synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from its promoter.
  • SP6 RNA Polymerase: Catalyzes the 5´→3´ synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from its promoter and incorporates modified nucleotides.
  • Anza T4 Polynucleotide Kinase: Transfers the terminal phosphate of ATP to the 5´ hydroxyl terminus of DNA or RNA. Used for 5´ end-labeling of oligonucleotides and polynucleotides.
  • T4 RNA Ligase: Catalyzes the formation of a phosphodiester linkage between a 5´-phosphoryl-terminated ribonucleic acid and a 3´-hydroxyl-terminated ribonucleic acid.

In vitro transcription kits
  • MEGAscript SP6T7, and T3 Kits: Synthesize ultra-high yields of RNA, 10 to 50 times as much as produced by conventional transcription reactions.

Other
  • Cap Analog & Variants: m7G(5´)ppp(5´)G (cap analog) and cap variants. The cap analog is used for synthesis of 5´-capped RNA molecules.
  • Modified UTPs: Incorporate these nucleotides to confer unique characteristics and test for particular reactive groups.

References