RNA Extraction for Downstream Applications
Purified RNA is used in many downstream applications including real-time RT-PCR, microarray analysis, next-generation RNA sequencing (RNA-Seq), northern blotting, and cloning.
Depending on the application, you may need to extract RNA from cellular material or biofluids, remove genomic DNA, or enrich for certain types of RNA.
Microarrays are important tools in molecular biology and genomics labs. Especially for transcriptomics (mRNA and microRNA), the microarray approach offers a streamlined method for analysis of thousands of transcripts in a single experiment.
Northern blot analysis is a common use for RNA. Used to determine mRNA identity, size, and relative abundance, northern blotting allows measurement of gene expression levels and transcript size as well as detection of alternatively spliced transcripts.
For Research Use Only. Not for use in diagnostic procedures.