mRNA extraction and enrichment kits for a variety of sample types

Our kits make mRNA extraction and enrichment easy. For one experiment you may need to quickly obtain high quantities of mRNA, while another experiment may demand the purest quality of mRNA. See below for our selection guide or learn more about other RNA extraction products.

Which mRNA purification kit is right for you?

The selection guide below is designed to help compare kits for different mRNA research applications.

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 Rapid mRNA purified from total RNANo purification needed, mRNA direct from crude samplesmRNA from micro-sized samples, best for next-generation sequencing of mRNAHTP mRNA directly or enrich from total RNA
 Dynabeads mRNA Purification Kit Dynabeads mRNA DIRECT Dynabeads mRNA DIRECT Micro Kit mRNA Catcher Plus
Top seller    
Prep time15 minutes15 minutes15 minutes1.5 hours
Direct isolation of mRNA from crude samplesNoYesYesYes
mRNA enrichment from total RNA YesNoNoYes
Amount of starting material<75 μg Total RNA<20 x 106 cells
2–200 mg animal tissue
4–400 mg plant tissue
<1 x 104 cells
<5 mg plant or animal tissue
100 to 1 x 106 cells
<4 mg tissue
40 µL whole blood
100 ng–100 µg total RNA
FormatFast magnetic bead capture from pure total RNAFast magnetic bead capture directly from crude samplesFast magnetic bead capture directly from micro-size samplesHTP-ready 96-well coated plate
High-throughput compatibleYesYesYesYes
Kit size2 mL oligo dT beads5 mL or 10 mL oligo dT beads2 mL oligo dT beads (sufficient for 100 mRNA preps)96 or 960 preps

Magnetic bead mRNA extraction and enrichment kits

Oligo(dT)/magnetic capture methods

Column methods for mRNA purification are reliable but require extensive manual interaction, which means more time and reduced throughput. Magnetic beads conjugated with oligo(dT) offer additional handling and scaling benefits; however, this procedure does require the use of magnetic stands.

The Invitrogen Ambion Poly(A)Purist MAG Kit employs the same hybridization and wash solutions as those in the Poly(A)Purist Kit but with the added ease, speed, and scalability of magnetic beads. The protocol typically takes only 45 minutes, and the kit performs from 8 large enrichment reactions (up to 1 mg total RNA input) to 80 small enrichment reactions (as little as 30 µg total RNA input). Like the Poly(A)Purist Kit, this kit only enriches mRNA from previously purified total RNA.

The Invitrogen Dynabeads mRNA Purification Kit for mRNA Purification from Total RNA Preps, enriches mRNA from purified total RNA, typically in 15 minutes, and allows users the flexibility to elute the mRNA from the beads in as little as 5 µL, or even to perform cDNA synthesis directly on the beads without elution.

For those who prefer using Dynabeads technology with sample lysis and enrichment in one kit, the Invitrogen Dynabeads mRNA DIRECT Kits enable 15-minute lysis and enrichment processing of a variety of sample types. In fact, it is possible to capture mRNA and generate cDNA libraries from a single cell using this technology. The flexible format allows users to scale up or down and gives the option to elute the mRNA from the beads in as little as 5 µL, or to perform cDNA synthesis directly on the beads without elution. The 5 mL size is sufficient for 20 standard isolations, while the 10 mL size is sufficient for 40 standard isolations. 

For those who work with smaller inputs, the Invitrogen Dynabeads mRNA DIRECT Micro Kit provides enough reagents for 100 mRNA isolations from up to 2.5 x 104 mononuclear cells, up to 1 x 104 cultured cells, or up to 5 mg tissue (depending on the tissue), with the same elution flexibility as the other Dynabeads kits.

Learn more about how you can isolate mRNA in 15 minutes

Bacteria lack the relatively stable poly(A) tails found on eukaryotic mRNA. Until very recently, isolating mRNA from bacteria has been virtually impossible. The Invitrogen MICROBExpress Bacterial mRNA Isolation Kit employs a novel technology to remove >95% of the 16S and 23S rRNA from total RNA of E. coli and other bacterial species. The kit is suitable for mRNA enrichment from ≤10 µg of purified bacterial total RNA in about 2 hours from a broad spectrum of gram-positive and gram-negative bacteria. mRNA isolated with the MICROBExpress Kit is a superior template for synthesizing labeled cDNA for array analysis and is ideal for quantitative RT-PCR, northern blotting, and cDNA library construction.

mRNA extraction and enrichment via coated plates

The most distinctive mRNA purification method takes advantage of specially treated plates in conjunction with optimized reagents to both lyse and enrich mRNA from cells, tissue, blood, or purified total RNA. The Invitrogen mRNA Catcher PLUS product utilizes a proprietary surface treatment of a 96-well plate to facilitate easy and automation-friendly mRNA purification. Locked Nucleic Acid (LNA) technology incorporated into the 20-mer oligo(dT) increases specificity and hybridization efficiency while removing centrifugation, precipitation, and phase-separation steps from the overall workflow.

The mRNA Catcher PLUS Kit is capable of handling 100 ng to 100 µg total RNA, 100 to 106 mammalian cells, up to 4 mg tissue, and 40 µL whole blood. The binding capacity is >80 ng/well. The protocol employs a simple lysis, hybridization, wash, and elution process that can be completely automated on open-format liquid handlers in approximately 1.5 hours from start to finish. The system also has the flexibility to elute mRNA or to perform cDNA synthesis directly in the hybridization plate without elution.

  • Protocol Videos—Videos to help you isolate nucleic acids using a variety of techniques.
  • DNA & RNA Selection Guide—Find the right purification products.
  • Videos—View our library of DNA & RNA purification and analysis videos.
  • Application Notes—Explore our application notes from scientists sharing data for isolation products

For Research Use Only. Not for use in diagnostic procedures.