mRNA Catcher™ PLUS Purification Kit

Catalog number: K157002

Ambion™  Related applications: Automated Nucleic Acid Purification | RNA Extraction

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The mRNA Catcher™ PLUS Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format. By taking advantage of Locked Nucleic Acid (LNA) technology, the mRNA Catcher™ PLUS Kit provides improved performance and a faster protocol, while preserving mRNA yield and purity (see table). The mRNA Catcher™ PLUS Kit offers:

Robust performance—high binding capacity per well (>80 ng/well)
Reproducible results—low CVs from well-to-well and plate-to-plate (~2%)
Optimized protocols—for small sample sizes (100 cells, 4 mg tissue, and 40 µL blood)
Compatible plate design—may be used with most PCR cyclers

How it works
mRNA from total RNA, cells, tissue, and blood samples is hybridized to an immobilized, single-stranded 20-mer oligo(dT)-containing LNA. The incorporation of LNA into the oligo probe increases the hybridization efficiency and specificity of the mRNA binding. The mRNA can either be eluted or directly reversed-transcribed to cDNA directly in the well. The mRNA Catcher™ PLUS method does not require centrifugation, precipitation, phase separation, or column filtration. In addition, the entire method is amenable to automation for streamlined, high-throughput expression profiling for large-scale processes. Isolated mRNA is suitable for use in downstream applications such as RT-PCR and qRT-PCR.
For Research Use Only. Not for use in diagnostic procedures.


Sample Type (General): RNA, Cells, Tissue, Blood
High Throughput Compatibility: Automated Protocols, High Throughput-Compatible
Downstream Application: Reverse Transcriptase PCR (RT-PCR), Real-Time Quantitative PCR (qPCR), Microarray Analysis, cDNA Library Construction
Format: 96-well plate
System: mRNA Catcher
Quantity: 1
Product Size: 96 preps
Final Product: mRNA
Purification Time: 1.5 hrs
Number of Reactions: 96 Preps
Elution Volume - max: 80 µl
Elution Volume - min: 80 µl
Isolation Technology: Oligo dT Affinity
Sample Type (Specific): Total RNA, Mammalian Cells, Whole Blood, Tissue (Mammalian)
For Use With (Equipment): Robotic Liquid Handling Systems
Shipping Condition: Wet Ice

Contents & storage

• 1 96-well mRNA Catcher™ PLUS plate (4°C)
• 4 ml 2X Lysis Buffer L20 (room temperature)
• 30 ml Wash Buffer W15 (room temperature)
• 4 ml Dilution Buffer DL1 (room temperature)
• 8 ml Elution Buffer E3 (room temperature)
• 100 µl 0.5M DTT (4°C)


Manuals & protocols

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