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Invitrogen™

SuperScript™ VILO™ cDNA Synthesis Kit

Catalog number: 11754050
Invitrogen™

SuperScript™ VILO™ cDNA Synthesis Kit

Catalog number: 11754050
Catalog Number
11754050
Unit Size
50 reactions
Price (USD)
Price: 694.00
Online price: 659.65
Your price:
Availability
-
Quantity
Catalog Number
11754250
also known as 11754-250
Unit Size
250 reactions
Price (USD)
Price: 3,055.00
Online price: 2,902.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
1175405050 reactions
Price: 694.00
Online price: 659.65
Your price:
-
11754250
also known as 11754-250
250 reactions
Price: 3,055.00
Online price: 2,902.65
Your price:
-
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The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis.

The SuperScript VILO cDNA Synthesis Kit can be used to synthesize cDNA from for a wide range of input RNA amounts. This kit provides the high temperature capability of SuperScript III Reverse Transcriptase in an optimized format for generating first-strand cDNA for use in RT–qPCR.

Notes
• For superior first-strand cDNA synthesis in two-step RT-qPCR, try SuperScript IV VILO Master Mix or SuperScript IV VILO Master Mix with ezDNase Enzyme.
• This kit is included with EXPRESS Two-Step RT–qPCR kits. Reagents are provided for 50 or 250 20-μL cDNA synthesis reactions.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
qPCR
Format
Kit
GC-Rich PCR Performance
High
Reaction Speed
60 min.
Technique
Reverse Transcription
Optimal Reaction Temperature
42°C
Reverse Transcriptase
SuperScript III
Ribonuclease H Activity
Reduced
Shipping Condition
Dry Ice
For Use With (Application)
Real Time PCR (qPCR)
Final Product Type
First-Strand cDNA
No. of Reactions
50 Reactions
Reaction Format
Master Mix
Reagent Type
Reverse Transcription
Size (Final Product)
Up to 10 kb
Starting Material
RNA

Contents & Storage

• 5X VILO Reaction Mix, 200 μL
• 10X SuperScript III Enzyme Blend, 100 μL

Store at –5 to –30°C.

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