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Invitrogen™

SuperScript™ VILO™ cDNA Synthesis Kit

Catalog number: 11754050
Invitrogen™

SuperScript™ VILO™ cDNA Synthesis Kit

Catalog number: 11754050
Catalog Number
11754050
also known as 11754-050
Unit Size
50 reactions
Price (USD)
642.00
Your Price:
Availability
***
Quantity
Catalog Number
11754250
also known as 11754-250
Unit Size
250 reactions
Price (USD)
2,850.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
11754050
also known as 11754-050
50 reactions
642.00
Your Price:
***
11754250
also known as 11754-250
250 reactions
2,850.00
Your Price:
***
Product Overview
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The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a 2-tube format with the VILO Reaction Mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis. The kit can be used to synthesize cDNA from for a wide range of input RNA amounts.

Note: For superior first-strand cDNA synthesis performance in two-step RT-qPCR, we recommend SuperScript IV VILO Master Mix or SuperScript IV VILO Master Mix with ezDNase Enzyme. The master mix provides all cDNA synthesis reaction components in a convenient one-tube master mix format. It elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV RT and further optimized buffer. The master mix enables efficient cDNA synthesis at higher temperatures in less time and provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)
Real-Time Quantitative PCR (qPCR)
No. of Tests
50 Reactions
Optimal Reaction Temperature
42° C
Primer
Random Primers
Product Line
SuperScript™, VILO™
Product Type
cDNA Synthesis Kit
Quantity
50 reactions
Reverse Transcriptase
SuperScript™ III
Ribonuclease H Activity
Reduced
Shipping Condition
Dry Ice
Sufficient For
50 Reactions
Concentration
10 X
Detection Method
Primer-Probe
Fidelity (vs. Taq)
10 X
Format
Tube
GC-Rich PCR Performance
High
No. of Reactions
50 Reactions
PCR Method
qPCR, RT-PCR
Reaction Format
SuperMix or Master Mix
Sample Type
RNA
Size (Final Product)
10 kb or less
Volume
20 μL

Contents & Storage

• 5X VILO Reaction Mix containing random primers, dNTPs, and MgCl2
• 10X SuperScript III Enzyme Blend containing SuperScript III RT, RNaseOUT Recombinant Ribonuclease Inhibitor,and proprietary helper protein

Store in freezer (-5 to -30°C).

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Frequently asked questions (FAQs)

Citations & References