Upgrade to Invitrogen SuperScript™ IV VILO™ Master Mix or SuperScript™ IV VILO™ Master Mix with ezDNase Enzyme—super-fast, super-efficient, and super-high yields.
Invitrogen™
SuperScript™ VILO™ cDNA Synthesis Kit
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Description
The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a 2-tube format with the VILO Reaction Mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis. The kit can be used to synthesize cDNA from for a wide range of input RNA amounts.Note: For superior first-strand cDNA synthesis performance in two-step RT-qPCR, we recommend SuperScript IV VILO Master Mix or SuperScript IV VILO Master Mix with ezDNase Enzyme. The master mix provides all cDNA synthesis reaction components in a convenient one-tube master mix format. It elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV RT and further optimized buffer. The master mix enables efficient cDNA synthesis at higher temperatures in less time and provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Reverse Transcriptase: | SuperScript™ III |
|---|---|
| Ribonuclease H Activity: | Reduced |
| Downstream Application: | Real-Time Quantitative PCR (qPCR) |
| Reaction Format: | SuperMix or Master Mix |
| Sample Type (General): | RNA |
| Final Product Size(s): | 10 kb or less |
| Optimal Reaction Temperature: | 42° C |
| Primer Type: | Random Primers |
| Product Line: | SuperScript™, VILO™ |
| Product Size: | 50 reactions |
| Shipping Condition: | Dry Ice |
Contents & storage
• 5X VILO Reaction Mix containing random primers, dNTPs, and MgCl2
• 10X SuperScript III Enzyme Blend containing SuperScript III RT, RNaseOUT Recombinant Ribonuclease Inhibitor, and proprietary helper protein
Store in freezer (-5 to -30°C).
• 10X SuperScript III Enzyme Blend containing SuperScript III RT, RNaseOUT Recombinant Ribonuclease Inhibitor, and proprietary helper protein
Store in freezer (-5 to -30°C).
