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인용 및 참조 문헌 (5)
Invitrogen™
SuperScript™ VILO™ cDNA Synthesis Kit
The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is자세히 알아보기
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보기 방식 변경
| 카탈로그 번호 | 반응 수 |
|---|---|
| 11754050 | 50 Reactions |
| 11754250 | 250 Reactions |
카탈로그 번호 11754050
제품 가격(KRW)
827,000
온라인 행사
Ends: 31-Mar-2026
972,000할인액 145,000 (15%)
Each
반응 수:
50 Reactions
제품 가격(KRW)
827,000
온라인 행사
Ends: 31-Mar-2026
972,000할인액 145,000 (15%)
Each
The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a 2-tube format with the VILO Reaction Mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis. The kit can be used to synthesize cDNA from for a wide range of input RNA amounts.
Note: For superior first-strand cDNA synthesis performance in two-step RT-qPCR, we recommend SuperScript IV VILO Master Mix or SuperScript IV VILO Master Mix with ezDNase Enzyme. The master mix provides all cDNA synthesis reaction components in a convenient one-tube master mix format. It elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV RT and further optimized buffer. The master mix enables efficient cDNA synthesis at higher temperatures in less time and provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates.
본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
Note: For superior first-strand cDNA synthesis performance in two-step RT-qPCR, we recommend SuperScript IV VILO Master Mix or SuperScript IV VILO Master Mix with ezDNase Enzyme. The master mix provides all cDNA synthesis reaction components in a convenient one-tube master mix format. It elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV RT and further optimized buffer. The master mix enables efficient cDNA synthesis at higher temperatures in less time and provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates.
본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
최종 제품 유형First-Strand cDNA
형식Kit
반응 수50 Reactions
최적 반응 온도42°C
수량50 reactions
반응 형식Master Mix
시약 유형Reverse Transcription
역전사 효소SuperScript III
리보뉴클레아제 H 활성Reduced
배송 조건Dry Ice
크기(최종 제품)Up to 10 kb
시작 물질RNA
기술Reverse Transcription
검출 방법qPCR
용도(애플리케이션)Real Time PCR (qPCR)
GC-Rich PCR PerformanceHigh
반응 속도60 min.
Unit SizeEach
구성 및 보관
• 5X VILO Reaction Mix, 200 μL
• 10X SuperScript III Enzyme Blend, 100 μL
Store at –5 to –30°C.
자주 묻는 질문(FAQ)
How long can I store the cDNA from my reverse transcription step?
What is the difference between SuperScript III RT and the RT in the SuperScript VILO kit?
How much of the first-strand cDNA reaction should I load for PCR?
In what concentration range of total RNA does the SuperScript VILO cDNA Synthesis Kit (Cat. No. 11754050) work?
What is the RNA input for the Oncomine Focus Assay, Chef-Ready Library kit?
인용 및 참조 문헌 (5)
인용 및 참조 문헌
Abstract
A useful approach to total analysis of RISC-associated RNA.
Journal:BMC Res Notes
PubMed ID:19706194
'ABSTRACT: BACKGROUND: Identifying the endogenous RNA induced silencing complex(RISC)-associated RNAs is essential for understanding the cellular regulatory networks by miRNAs. Recently, isolation of RISC-associated mRNAs using antibody was reported, but their method needs a large amount of initial materials. We tried to improve the protocol and constructed an efficient and
Transcriptome analysis of proliferating Arabidopsis endosperm reveals biological implications for the control of syncytial division, cytokinin signaling, and gene expression regulation.
Journal:Plant Physiol
PubMed ID:18923020
During the early stages of seed development, Arabidopsis (Arabidopsis thaliana) endosperm is syncytial and proliferates rapidly through repeated rounds of mitosis without cytokinesis. This stage of endosperm development is important in determining final seed size and is a model for studying aspects of cellular and molecular biology, such as the
T cells modulate Epstein-Barr virus latency phenotypes during infection of humanized mice.
Journal:
PubMed ID:24390326
Human B cells, the main target of Epstein-Barr virus (EBV), can display several types of latent viral protein expression, denoted 0, I, IIa, IIb, or III. Of these, only type III expression induces proliferation of cells in vitro. These latency types are present at specific stages of infection and are
Decrease of miR-146b-5p in monocytes during obesity is associated with loss of the anti-inflammatory but not insulin signaling action of adiponectin.
Journal:PLoS One
PubMed ID:22393448
Low adiponectin, a well-recognized antidiabetic adipokine, has been associated with obesity-related inflammation, oxidative stress and insulin resistance. Globular adiponectin is an important regulator of the interleukin-1 receptor-associated kinase (IRAK)/NF?B pathway in monocytes of obese subjects. It protects against inflammation and oxidative stress by inducing IRAK3. microRNA (miR)-146b-5p inhibits NF?B-mediated inflammation
Cyclin D1 splice variants: polymorphism, risk, and isoform-specific regulation in prostate cancer.
Journal:Clin Cancer Res
PubMed ID:19706803
PURPOSE: Alternative CCND1 splicing results in cyclin D1b, which has specialized, protumorigenic functions in prostate not shared by the cyclin D1a (full length) isoform. Here, the frequency, tumor relevance, and mechanisms controlling cyclin D1b were challenged. EXPERIMENTAL DESIGN: First, relative expression of both cyclin D1 isoforms was determined in prostate