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Invitrogen™

SuperScript™ VILO™ cDNA Synthesis Kit

Catalog number: 11754050
Invitrogen™

SuperScript™ VILO™ cDNA Synthesis Kit

Catalog number: 11754050
Catalog Number
11754050
Unit Size
50 reactions
Price (USD)
Price: 682.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
250 reactions
Price (USD)
Price: 3,025.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
1175405050 reactions
Price: 682.00
Your Price:
-
11754250250 reactions
Price: 3,025.00
Your Price:
-
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The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a 2-tube format with the VILO Reaction Mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis. The kit can be used to synthesize cDNA from for a wide range of input RNA amounts.

Note: For superior first-strand cDNA synthesis performance in two-step RT-qPCR, we recommend SuperScript IV VILO Master Mix or SuperScript IV VILO Master Mix with ezDNase Enzyme. The master mix provides all cDNA synthesis reaction components in a convenient one-tube master mix format. It elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV RT and further optimized buffer. The master mix enables efficient cDNA synthesis at higher temperatures in less time and provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
qPCR
Final Product Type
First-Strand cDNA
Format
cDNA Synthesis Kit
GC-Rich PCR Performance
High
No. of Reactions
50 Reactions
PCR Method
RT-qPCR
Reaction Format
Master Mix
Reaction Speed
60 min.
Reagent Type
Reverse Transcription
Size (Final Product)
Up to 10 kb
Starting Material
RNA
Technique
Reverse Transcription
Volume
20 μL
Optimal Reaction Temperature
42°C
Reverse Transcriptase
SuperScript III
Ribonuclease H Activity
Reduced
Shipping Condition
Dry Ice
For Use With (Application)
Real-Time Quantitative PCR (qPCR)

Contents & Storage

• 5X VILO Reaction Mix
• 10X SuperScript III Enzyme Blend

Store at –30°C to –5°C.