SuperScript™ VILO™ cDNA Synthesis Kit
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SuperScript™ VILO™ cDNA Synthesis Kit
Invitrogen™

SuperScript™ VILO™ cDNA Synthesis Kit

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit isRead more
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Catalog NumberNo. of Reactions
11754250250 Reactions
1175405050 Reactions
Catalog number 11754250
Price (USD)/ Each
2,960.65
Online exclusive
3,175.00 
Save 214.35 (7%)
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Add to cart
No. of Reactions:
250 Reactions
Request bulk or custom format
Price (USD)/ Each
2,960.65
Online exclusive
3,175.00 
Save 214.35 (7%)
Add to cart
SuperScript™ VILO™ cDNA Synthesis Kit
Catalog number11754250
Price (USD)/ Each
2,960.65
Online exclusive
3,175.00 
Save 214.35 (7%)
-
Add to cart

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis.

The SuperScript VILO cDNA Synthesis Kit can be used to synthesize cDNA from for a wide range of input RNA amounts. This kit provides the high temperature capability of SuperScript III Reverse Transcriptase in an optimized format for generating first-strand cDNA for use in RT–qPCR.

Notes
• For superior first-strand cDNA synthesis in two-step RT-qPCR, try SuperScript IV VILO Master Mix or SuperScript IV VILO Master Mix with ezDNase Enzyme.
• This kit is included with EXPRESS Two-Step RT–qPCR kits. Reagents are provided for 50 or 250 20-μL cDNA synthesis reactions.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodqPCR
FormatKit
GC-Rich PCR PerformanceHigh
Reaction Speed60 min.
TechniqueReverse Transcription
Optimal Reaction Temperature42°C
Quantity250 reactions
Reverse TranscriptaseSuperScript III
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR)
Final Product TypeFirst-Strand cDNA
No. of Reactions250 Reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Size (Final Product)Up to 10 kb
Starting MaterialRNA
Unit SizeEach
Contents & Storage

• 5X VILO Reaction Mix, 1,000 μL
• 10X SuperScript III Enzyme Blend, 500 μL

Store at –5 to –30°C.