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Invitrogen™

SuperScript™ VILO™ cDNA Synthesis Kit

Catalog number:  11754250

Related applications:

Reverse Transcription

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Description

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a 2-tube format with the VILO Reaction Mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis. The kit can be used to synthesize cDNA from for a wide range of input RNA amounts.

Note: For superior first-strand cDNA synthesis performance in two-step RT-qPCR, we recommend SuperScript IV VILO Master Mix or SuperScript IV VILO Master Mix with ezDNase Enzyme. The master mix provides all cDNA synthesis reaction components in a convenient one-tube master mix format. It elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV RT and further optimized buffer. The master mix enables efficient cDNA synthesis at higher temperatures in less time and provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application): Real-Time Quantitative PCR (qPCR)
Sample Type: RNA
Primer: Random Primers
Quantity: 250 reactions
Size (Final Product): 10 kb or less
Optimal Reaction Temperature: 42° C
Reaction Format: SuperMix or Master Mix
Reverse Transcriptase: SuperScript™ III
Ribonuclease H Activity: Reduced
Shipping Condition: Dry Ice
Product Line: SuperScript™, VILO™

Contents & storage

• 5X VILO Reaction Mix containing random primers, dNTPs, and MgCl2
• 10X SuperScript III Enzyme Blend containing SuperScript III RT, RNaseOUT Recombinant Ribonuclease Inhibitor,and proprietary helper protein

Store in freezer (-5 to -30°C).