Informational:Inquire about OEM or Commercial Supply version of this product here.
Informational:Upgrade to Invitrogen SuperScript™ IV VILO™ Master Mix or SuperScript™ IV VILO™ Master Mix with ezDNase Enzyme—super-fast, super-efficient, and super-high yields.
Invitrogen™

SuperScript™ VILO™ cDNA Synthesis Kit

Catalog number: 11754250
Invitrogen™

SuperScript™ VILO™ cDNA Synthesis Kit

Catalog number: 11754250

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis.

The SuperScript VILO cDNA Synthesis Kit can be used to synthesize cDNA from for a wide range of input RNA amounts. This kit provides the high temperature capability of SuperScript III Reverse Transcriptase in an optimized format for generating first-strand cDNA for use in RT–qPCR.

Notes
• For superior first-strand cDNA synthesis in two-step RT-qPCR, try SuperScript IV VILO Master Mix or SuperScript IV VILO Master Mix with ezDNase Enzyme.
• This kit is included with EXPRESS Two-Step RT–qPCR kits. Reagents are provided for 50 or 250 20-μL cDNA synthesis reactions.

 
Catalog Number
11754250
also known as 11754-250
Unit Size
250 reactions
No. of Reactions
250 Reactions
Price (USD)
Price: 3,055.00
Online price: 2,902.65
Your price:
Availability
-
Full specifications
Quantity
Detection MethodqPCR
FormatKit
GC-Rich PCR PerformanceHigh
Reaction Speed60 min.
TechniqueReverse Transcription
Optimal Reaction Temperature42°C
Reverse TranscriptaseSuperScript III
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR)
Final Product TypeFirst-Strand cDNA
No. of Reactions250 Reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Size (Final Product)Up to 10 kb
Starting MaterialRNA
Unit Size250 reactions
Catalog Number
Unit Size
50 reactions
No. of Reactions
50 Reactions
Price (USD)
Price: 694.00
Online price: 659.65
Your price:
Availability
-
Full specifications
Quantity
Detection MethodqPCR
FormatKit
GC-Rich PCR PerformanceHigh
Reaction Speed60 min.
TechniqueReverse Transcription
Optimal Reaction Temperature42°C
Reverse TranscriptaseSuperScript III
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR)
Final Product TypeFirst-Strand cDNA
No. of Reactions50 Reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Size (Final Product)Up to 10 kb
Starting MaterialRNA
Unit Size50 reactions
Showing 2 of 2
Catalog NumberSpecificationsUnit SizeNo. of ReactionsPrice (USD)AvailabilityQuantity
11754250
also known as 11754-250
Full specifications
250 reactions250 Reactions
Price: 3,055.00
Online price: 2,902.65
Your price:
-
Detection MethodqPCR
FormatKit
GC-Rich PCR PerformanceHigh
Reaction Speed60 min.
TechniqueReverse Transcription
Optimal Reaction Temperature42°C
Reverse TranscriptaseSuperScript III
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR)
Final Product TypeFirst-Strand cDNA
No. of Reactions250 Reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Size (Final Product)Up to 10 kb
Starting MaterialRNA
Unit Size250 reactions
11754050Full specifications
50 reactions50 Reactions
Price: 694.00
Online price: 659.65
Your price:
-
Detection MethodqPCR
FormatKit
GC-Rich PCR PerformanceHigh
Reaction Speed60 min.
TechniqueReverse Transcription
Optimal Reaction Temperature42°C
Reverse TranscriptaseSuperScript III
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR)
Final Product TypeFirst-Strand cDNA
No. of Reactions50 Reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Size (Final Product)Up to 10 kb
Starting MaterialRNA
Unit Size50 reactions
Showing 2 of 2

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis.

The SuperScript VILO cDNA Synthesis Kit can be used to synthesize cDNA from for a wide range of input RNA amounts. This kit provides the high temperature capability of SuperScript III Reverse Transcriptase in an optimized format for generating first-strand cDNA for use in RT–qPCR.

Notes
• For superior first-strand cDNA synthesis in two-step RT-qPCR, try SuperScript IV VILO Master Mix (Cat. No. 11756050) or SuperScript IV VILO Master Mix with ezDNase Enzyme (Cat. No. 11766050).
• This kit is included with EXPRESS Two-Step RT–qPCR kits. Reagents are provided for 50 or 250 20-μL cDNA synthesis reactions.

For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

• 5X VILO Reaction Mix, 1,000 μL
• 10X SuperScript III Enzyme Blend, 500 μL

Store at –5 to –30°C.

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text