Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis.
The SuperScript VILO cDNA Synthesis Kit can be used to synthesize cDNA from for a wide range of input RNA amounts. This kit provides the high temperature capability of SuperScript III Reverse Transcriptase in an optimized format for generating first-strand cDNA for use in RT–qPCR.
Notes
• For superior first-strand cDNA synthesis in two-step RT-qPCR, try SuperScript IV VILO Master Mix or SuperScript IV VILO Master Mix with ezDNase Enzyme.
• This kit is included with EXPRESS Two-Step RT–qPCR kits. Reagents are provided for 50 or 250 20-μL cDNA synthesis reactions.
Detection Method | qPCR |
Format | Kit |
GC-Rich PCR Performance | High |
Reaction Speed | 60 min. |
Technique | Reverse Transcription |
Optimal Reaction Temperature | 42°C |
Reverse Transcriptase | SuperScript III |
Ribonuclease H Activity | Reduced |
Shipping Condition | Dry Ice |
For Use With (Application) | Real Time PCR (qPCR) |
Final Product Type | First-Strand cDNA |
No. of Reactions | 250 Reactions |
Reaction Format | Master Mix |
Reagent Type | Reverse Transcription |
Size (Final Product) | Up to 10 kb |
Starting Material | RNA |
Unit Size | 250 reactions |
Detection Method | qPCR |
Format | Kit |
GC-Rich PCR Performance | High |
Reaction Speed | 60 min. |
Technique | Reverse Transcription |
Optimal Reaction Temperature | 42°C |
Reverse Transcriptase | SuperScript III |
Ribonuclease H Activity | Reduced |
Shipping Condition | Dry Ice |
For Use With (Application) | Real Time PCR (qPCR) |
Final Product Type | First-Strand cDNA |
No. of Reactions | 50 Reactions |
Reaction Format | Master Mix |
Reagent Type | Reverse Transcription |
Size (Final Product) | Up to 10 kb |
Starting Material | RNA |
Unit Size | 50 reactions |
Catalog Number | Specifications | Unit Size | No. of Reactions | Price (USD) | Availability | Quantity | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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11754250 also known as 11754-250 | 250 reactions | 250 Reactions | Price: 3,055.00 Online price: 2,902.65 Your price: | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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11754050 | 50 reactions | 50 Reactions | Price: 694.00 Online price: 659.65 Your price: | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis.
The SuperScript VILO cDNA Synthesis Kit can be used to synthesize cDNA from for a wide range of input RNA amounts. This kit provides the high temperature capability of SuperScript III Reverse Transcriptase in an optimized format for generating first-strand cDNA for use in RT–qPCR.
Notes
• For superior first-strand cDNA synthesis in two-step RT-qPCR, try SuperScript IV VILO Master Mix (Cat. No. 11756050) or SuperScript IV VILO Master Mix with ezDNase Enzyme (Cat. No. 11766050).
• This kit is included with EXPRESS Two-Step RT–qPCR kits. Reagents are provided for 50 or 250 20-μL cDNA synthesis reactions.
• 5X VILO Reaction Mix, 1,000 μL
• 10X SuperScript III Enzyme Blend, 500 μL
Store at –5 to –30°C.