Labeled and unlabeled tubulin. Tetramethylrhodamine-labeled tubulin and unlabeled tubulin.

Tetramethylrhodamine-labeled tubulin (T7460) copolymerized with unlabeled tubulin (T7451) in G-PEM buffer (80 mM PIPES, 1 mM MgCl2 and 1 mM GTP). After 10 minutes, the polymerized tubulin was diluted 1:1000 with G-PEM buffer containing 60% glycerol and 0.1% glutaraldehyde and photographed through a longpass filter set appropriate for tetramethylrhodamine.

Tetramethylrhodamine-labeled tubulin (T7460) copolymerized with unlabeled tubulin (T7451) in G-PEM buffer (80 mM PIPES, 1 mM MgCl<sub>2</sub> and 1 mM GTP). After 10 minutes, the polymerized tubulin was diluted 1:1000 with G-PEM buffer containing 60% glycerol and 0.1% glutaraldehyde and photographed through a longpass filter set appropriate for tetramethylrhodamine.

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