Fragment Library Construction Kit for SOLiD® Next-Generation Sequencing
The SOLiD® Fragment Library Construction Kit provides a fully optimized set of reagents for constructing 10 fragment libraries for use with the SOLiD® Next Generation Sequencing System.
Reach your sequencing goals with confidence
The kit is available in 3 configurations (Table 1). The SOLiD® Fragment Library Construction Kit with SizeSelect™ Gels contains reagents, columns, and gels for use with the SOLiD® standard protocol. The SOLiD® Fragment Library Construction Kit for use with the express protocol includes all reagents and columns. The SOLiD® Fragment Library Construction Reagents Kit contains no purification components.
The SOLiD® Fragment Library Construction Kit:
- Enables flexibility—choice of 3 kit configurations for use with standard or express SOLiD® protocols
- The optimized and recommended protocol for use with the SOLiD® System
- Components quality-tested together to enable optimal results
How SOLiD® Fragment Library Construction works
Fragment libraries consist of short lengths of DNA specifically selected for optimal performance with a next generation sequencing platform. Briefly, genomic DNA is sheared, primer adaptors are ligated to the fragmented DNA followed by an end-polishing step, and the library is amplified using primers specific to the adaptors (Figure 1). The fragments are size-selected, purified, and are then ready for the sequencing reactions.
The SOLiD® Fragment Library Construction Kit is specifically optimized for use with the SOLiD® Sequencing System.
|Product||Part Number||SOLiD® Fragment Library Construction Reagents||PureLink™ PCR Purification Kit (A11199)||E-Gel® SizeSelect 2% 10 Pack|
|SOLiD® Fragment Library Construction Kit
with SizeSelect™ Gels
|SOLiD® Fragment Library Construction Kit||4443473||Yes||Yes||No|
|SOLiD® Fragment Library Construction Reagents||4443713||Yes||No||No|
|Note: The SOLiD® Fragment Library Oligo Kit (Cat. No. 4401151) is not included and must be purchased separately. For research use only. Not intended for animal or human therapeutic or diagnostic use, unless otherwise indicated.|
SOLiD® Software For Your Application
- SOLiD® BioScope™ Software