DNA Barcoding Kits for SOLiD® Next-Generation Sequencing
Utilize the tremendous sequencing capacity of SOLiD® System with library multiplexing.
Reach Your DNA Sequencing Goals with Maximum Efficiency
SOLiD® DNA Barcoding Kits are currently available for SOLiD® fragment libraries.
SOLiD® Fragment Library Barcoding kits allow SOLiD® System users to multiplex up to 96 library samples on a single slide with the use of molecular barcodes. Multiplexing enables the pooling of samples prior to emulsion PCR (ePCR) on the SOLiD® System, which enables significant decreases in the cost and handling requirements of ePCR, enrichment, and deposition by allowing more samples per run. Such capabilities substantially benefit applications such as whole genome sequencing of small genomes or targeted resequencing of enriched samples.
Molecular barcodes are 10-base sequences embedded within the SOLiD® system sequencing adaptors (Figure 1), which are used to match sequence reads to original samples when read by the SOLiD® system. These specific DNA sequences are ligated directly to respective fragment libraries. The SOLiD® Fragment Library Barcoding Modules provide the necessary reagents to barcode fragment libraries from 8 µg input genomic DNA when used with SOLiD® Fragment Library Construction Kits.
Since SOLiD® Fragment Library Barcoding Kits follow the SOLiD® Fragment Library Construction protocol, it is easy to integrate barcodes in your fragment library construction workflow (figure 2). With the availability of 6 modules, each containing 16 unique barcodes, it is possible to follow this workflow for up to 96 samples.