RNA Barcoding Kits for SOLiD® Next-Generation Sequencing
Utilize the tremendous sequencing capacity of SOLiD® System with library multiplexing.
Reach Your RNA sequencing Goals with Maximum Efficiency
- Multiplex whole transcriptome, small-RNA, and SAGE™ libraries
- Unique barcode sequences provide optimal multiplexing
- Three kits available
Barcoding in RNA applications is advantageous when examining differentially expressed genes within a time course series or between drug treatments. SOLiD® RNA Barcoding Kits conveniently multiplex whole transcriptome and small RNA libraries constructed by the SOLiD® Total RNA-Seq Kit, and libraries constructed by the SOLiD® SAGE™ Kit.
Using unique barcode sequences for optimal multiplexing of up to 48 libraries, SOLiD® RNA Barcoding Kits enable the assignment of a unique identifier to templated beads made from a single library. Once the identifiers are assigned, multiple batches of templated beads may be pooled together for emulsion PCR and then sequenced.
Three kits are available. Each contains 16 different barcodes selected for
- Uniform melting temperature
- Low error rate
- Orthogonal sequences unique in color space
Barcodes are added to the target sequence 3' end using a modified P2 adapter (figure 1). SOLiD® RNA Barcoding Kits are compatible with SOLiD® Total RNA-Seq and SOLiD® SAGE™ protocols, making it easy to integrate barcodes during library construction (figure 2).
Following sequencing of the target, additional rounds of ligation-based sequencing are performed using primer sets complimentary to the barcode. The resulting reads are sorted by the barcode and aligned in groups to the reference sequence, enabling sequencing of multiple libraries on the same slide—maximizing throughput while dramatically reducing costs.