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Invitrogen
VEGF Receptor 3 Monoclonal Antibody (AFL4), eBioscience™
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FIGURE: 1 / 12
VEGF Receptor 3 Antibody (14-5988-82) in IHC
Life science alliance 2022 -
Figure S5. Increased Vegfr3 expression induced by Tbx1 Cre activation of TgVegfr3 is revealed by qRT-PCR but not by immunofluorescence. (A, B, C, D, E, F, G, H, I, J, K, L) Representative coronal brain sections of Tbx1 Cre/ + (A, B, C, D, E, F) and TgVegfr3; Tbx1 Cre/ + (G, H, I, J, K, L) embryos (E18.5) stained for VEGFR3 (red), VEGFR2 (green), and GFP ( Tbx1 cell fate, white). The cartoon shows the positi... View More
Product Details
14-5988-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
AFL4
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The AFL4 monoclonal antibody reacts with the mouse VEGF receptor-3, also known as Flt-4. This 195 kDa molecule was identified as an endothelial-specific member of the receptor tyrosine kinase (RTK) family. During early embryogenesis all endothelial cells express VEGFR-3, while in the adult tissues, VEGFR-3 expression disappears from the vascular endothelial cells and is observed only on the lymphatic endothelium. However, VEGFR-3 expression is induced in the adult tissue upon tumor implementation suggesting an important role for this receptor in the tumor angiogenesis. VEGF-C and VEGF-D bind to and activate VEGFR-3. AFL4 is an antagonist mAb.
Applications Reported: The AFL4 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections. It has also been reported in blocking of ligand binding. (Please use Functional Grade purified AFL4, Product # 16-5988, in functional assays).
Applications Tested: The AFL4 antibody has been tested by flow cytometric analysis of in vitro differentiated mouse endothelial cells. In brief, mouse ES cells were incubated on collagen IV matrix for 4 days and subsequently stimulated with VEGF under serum free conditions to induce further differentiation. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Vascular endothelial growth factor is a key regulator of blood vessel development in embryos and angiogenesis in adult tissues. Unlike VEGF, the related VEGFC stimulates the growth of lymphatic vessels through its specificl ymphatic endothelial receptor VEGFR-3. In 1998, Dumont showed that targeted inactivation of the VEGFR-3 gene in mice resulted in defective blood vessel development in early embryos. Vasculogenesis and angiogenesis occurred, but large vessels became abnormally organized with defective lumens, leading to fluid accumulation in the pericardial cavity and cardiovascular failure at embryonic day 9.5. Thus, VEGFR3 has an essential role in the development of theembryonic cardiovascular system before the emergence of the lymphatic vessels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: chylous ascites; FLT-4; Fms-like tyrosine kinase 4; receptor protein tyrosine kinase; soluble VEGFR 3; sVEGFR 3; Tyrosine-protein kinase receptor FLT4; Vascular endothelial growth factor receptor 3; vascular endothelial growth factor receptor-3; VEGF R3; VEGFR-3
Gene Aliases: AI323512; Chy; Flt-4; Flt4; VEGFR-3; VEGFR3
UniProt ID: (Mouse) P35917
Entrez Gene ID: (Mouse) 14257
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
vascular endothelial growth factor-activated receptor activity
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
growth factor binding
protein phosphatase binding
transmembrane signal receptor
angiogenesis
positive regulation of protein phosphorylation
positive regulation of endothelial cell proliferation
vasculature development
lymph vessel development
lymphangiogenesis
sprouting angiogenesis
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of cell proliferation
positive regulation of vascular endothelial growth factor production
positive regulation of endothelial cell migration
phosphorylation
peptidyl-tyrosine phosphorylation
positive regulation of cell growth
cellular response to vascular endothelial growth factor stimulus
vascular endothelial growth factor signaling pathway
negative regulation of apoptotic process
positive regulation of MAPK cascade
positive regulation of JNK cascade
protein autophosphorylation
vascular endothelial growth factor receptor signaling pathway
blood vessel morphogenesis
regulation of blood vessel remodeling
positive regulation of ERK1 and ERK2 cascade
positive regulation of protein kinase C signaling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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