Invitrogen

VEGF Receptor 3 Monoclonal Antibody (AFL4), Functional Grade, eBioscience™

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VEGF Receptor 3 Antibody — Published figure using VEGF Receptor 3 monoclonal antibody (Product # 16-5988-85)

Product Details

Tested Applications

Dilution

Control (Ctrl)

Assay-Dependent

Flow Cytometry (Flow)

Assay-Dependent

Functional Assay (FN)

Assay-Dependent

Published Applications

Western Blot (WB)

See 4 publications below

Flow Cytometry (Flow)

See 1 publication below

Immunohistochemistry (IHC)

See 2 publications below

Immunofluorescence (IF)

See 1 publication below

Miscellaneous PubMed (MISC)

See 1 publication below

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host / Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

AFL4

Conjugate

Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

RRID

AB_469192

Product Specific Information

Description: The AFL4 monoclonal antibody reacts with the mouse VEGF receptor-3, also known as Flt-4. This 195 kDa molecule was identified as an endothelial-specific member of the receptor tyrosine kinase (RTK) family. During early embryogenesis all endothelial cells express VEGFR-3, while in the adult tissues, VEGFR-3 expression disappears from the vascular endothelial cells and is observed only on the lymphatic endothelium. However, VEGFR-3 expression is induced in the adult tissue upon tumor implementation suggesting an important role for this receptor in the tumor angiogenesis. VEGF-C and VEGF-D bind to and activate VEGFR-3. AFL4 is an antagonist mAb.

Applications Reported: The AFL4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The AFL4 antibody has been tested by flow cytometric analysis of in vitro differentiated mouse endothelial cells. In brief, mouse ES cells were incubated on collagen IV matrix for 4 days and subsequently stimulated with VEGF under serum free conditions to induce further differentiation. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

Vascular endothelial growth factor is a key regulator of blood vessel development in embryos and angiogenesis in adult tissues. Unlike VEGF, the related VEGFC stimulates the growth of lymphatic vessels through its specificl ymphatic endothelial receptor VEGFR-3. In 1998, Dumont showed that targeted inactivation of the VEGFR-3 gene in mice resulted in defective blood vessel development in early embryos. Vasculogenesis and angiogenesis occurred, but large vessels became abnormally organized with defective lumens, leading to fluid accumulation in the pericardial cavity and cardiovascular failure at embryonic day 9.5. Thus, VEGFR3 has an essential role in the development of theembryonic cardiovascular system before the emergence of the lymphatic vessels.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: chylous ascites; FLT-4; Fms-like tyrosine kinase 4; receptor protein tyrosine kinase; Tyrosine-protein kinase receptor FLT4; Vascular endothelial growth factor receptor 3; vascular endothelial growth factor receptor-3; VEGFR-3

Gene Aliases: AI323512; Chy; Flt-4; Flt4; VEGFR-3; VEGFR3

UniProt ID: (Mouse) P35917

Entrez Gene ID: (Mouse) 14257

Function(s)

Process(es)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity vascular endothelial growth factor-activated receptor activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups growth factor binding protein phosphatase binding

VEGF Receptor 3 Monoclonal Antibody (AFL4), Functional Grade, eBioscience™

Product Details

Product Specific Information

Target Information

Bioinformatics

Material safety data sheets (MSDS)

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