Invitrogen

VEGF Receptor 3 Monoclonal Antibody (AFL4), Functional Grade, eBioscience™

Product Image Gallery

VEGF Receptor 3 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P)) — Published figure using VEGF Receptor 3 monoclonal antibody (Product # 16-5988-85) in Immunohistochemistry and Immunohistochemistry (Paraffin)

VEGF Receptor 3 Antibody in Immunohistochemistry (IHC) — Published figure using VEGF Receptor 3 monoclonal antibody (Product # 16-5988-85) in Immunohistochemistry

VEGF Receptor 3 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow) — Published figure using VEGF Receptor 3 monoclonal antibody (Product # 16-5988-85) in Immunohistochemistry and Flow Cytometry

VEGF Receptor 3 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Figure 4 Fbxw7 controls endothelial Notch signaling. Protein extracts prepared from P6 control (lanes 1 & 3 from the left) and Fbxw7 iECKO lungs (lanes 2 & 4) showed increased amounts of active Notch1 (NICD-Val1744) and reduced VEGFR3 protein (A). VE-Cadherin (Cdh5) was used for normalization. Relative Fbxw7 mRNA expression in HUVECs transfected with siFbxw7 relative to siControl siRNAs (n = 3 per group) (B). Human GAPDH was used for normalization. VEGF-A stimulation (50 ng/ml for the indicated time periods) led to a transient accumulation of active Notch (NICD) in HUVECs transfected with siControl . NICD levels were upregulated after Fbxw7 silencing (C). Western blot data (band intensity in pixels) was quantified with ImageJ; the graph shows average values from 3 independent experiments (D). Alpha-tubulin was used for normalization. Confocal images showing the pronounced reduction of VEGFR3 protein (green) in the endothelium (Isolectin B4; red) of the P6 Fbxw7 iECKO retina (E). Scale bar is 100 um. Quantitative analysis (with Volocity 5; n = 3 for each group) of image data confirmed reduced VEGFR3 levels (number of pixels) in mutant ECs (F). Error bars (in D and F) indicate SEM. P values are indicated as ** (<0.001) and * (p<0.05).

VEGF Receptor 3 Antibody in Western Blot (WB) — Published figure using VEGF Receptor 3 monoclonal antibody (Product # 16-5988-85) in Western Blot

VEGF Receptor 3 Antibody in Flow Cytometry (Flow) — Published figure using VEGF Receptor 3 monoclonal antibody (Product # 16-5988-85) in Flow Cytometry

Product Details

16-5988-85

Applications	Tested Dilution	Publications
Western Blot (WB)	-	View 5 publications 5 publications
Immunohistochemistry (IHC)	-	View 8 publications 8 publications
Immunohistochemistry (Paraffin) (IHC (P))	-	View 1 publication 1 publication
Flow Cytometry (Flow)	Assay-Dependent	View 2 publications 2 publications
Functional Assay (FN)	Assay-Dependent	-
Control (Ctrl)	Assay-Dependent	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Rat / IgG2a, kappa
Recommended Isotype Control	Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	AFL4
Conjugate	Functional Grade Biotin Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	no preservative
Storage conditions	4° C
RRID	AB_469192

Product Specific Information

Description: The AFL4 monoclonal antibody reacts with the mouse VEGF receptor-3, also known as Flt-4. This 195 kDa molecule was identified as an endothelial-specific member of the receptor tyrosine kinase (RTK) family. During early embryogenesis all endothelial cells express VEGFR-3, while in the adult tissues, VEGFR-3 expression disappears from the vascular endothelial cells and is observed only on the lymphatic endothelium. However, VEGFR-3 expression is induced in the adult tissue upon tumor implementation suggesting an important role for this receptor in the tumor angiogenesis. VEGF-C and VEGF-D bind to and activate VEGFR-3. AFL4 is an antagonist mAb.

Applications Reported: The AFL4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The AFL4 antibody has been tested by flow cytometric analysis of in vitro differentiated mouse endothelial cells. In brief, mouse ES cells were incubated on collagen IV matrix for 4 days and subsequently stimulated with VEGF under serum free conditions to induce further differentiation. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

Vascular endothelial growth factor is a key regulator of blood vessel development in embryos and angiogenesis in adult tissues. Unlike VEGF, the related VEGFC stimulates the growth of lymphatic vessels through its specificl ymphatic endothelial receptor VEGFR-3. In 1998, Dumont showed that targeted inactivation of the VEGFR-3 gene in mice resulted in defective blood vessel development in early embryos. Vasculogenesis and angiogenesis occurred, but large vessels became abnormally organized with defective lumens, leading to fluid accumulation in the pericardial cavity and cardiovascular failure at embryonic day 9.5. Thus, VEGFR3 has an essential role in the development of theembryonic cardiovascular system before the emergence of the lymphatic vessels.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Endothelial cells in the innate response to allergens and initiation of atopic asthma.

The Journal of clinical investigation

Asosingh K,Weiss K,Queisser K,Wanner N,Yin M,Aronica M,Erzurum S

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 16-5988-85) in Immunohistochemistry

Mon Jul 02 00:00:00 EDT 2018

G-Protein-Coupled Receptor-2-Interacting Protein-1 Controls Stalk Cell Fate by Inhibiting Delta-like 4-Notch1 Signaling.

Cell reports

Majumder S,Zhu G,Xu X,Senchanthisai S,Jiang D,Liu H,Xue C,Wang X,Coia H,Cui Z,Smolock EM,Libby RT,Berk BC,Pang J

16-5988 was used in Western Blotting to show that GIT1 inhibits the Notch1-Dll4 signaling pathway by competing with Notch1 ANK domain for binding to RBP-J in stalk cells.

Tue Dec 06 00:00:00 EST 2016

G-Protein-Coupled Receptor-2-Interacting Protein-1 Controls Stalk Cell Fate by Inhibiting Delta-like 4-Notch1 Signaling.

Cell reports

Majumder S,Zhu G,Xu X,Senchanthisai S,Jiang D,Liu H,Xue C,Wang X,Coia H,Cui Z,Smolock EM,Libby RT,Berk BC,Pang J

16-5988 was used in Western Blotting to show that GIT1 inhibits the Notch1-Dll4 signaling pathway by competing with Notch1 ANK domain for binding to RBP-J in stalk cells.

Tue Dec 06 00:00:00 EST 2016

Lymphatic Contribution to the Cellular Niche in Heterotopic Ossification.

Annals of surgery

Loder S,Agarwal S,Sorkin M,Breuler C,Li J,Peterson J,Gardenier J,Hsieh HH,Wang SC,Mehrara BJ,Levi B

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 16-5988-85) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

VEGF-D promotes pulmonary oedema in hyperoxic acute lung injury.

The Journal of pathology

Sato T,Paquet-Fifield S,Harris NC,Roufail S,Turner DJ,Yuan Y,Zhang YF,Fox SB,Hibbs ML,Wilkinson-Berka JL,Williams RA,Stacker SA,Sly PD,Achen MG

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 16-5988-85) in Immunohistochemistry

Wed Jun 01 00:00:00 EDT 2016

A Selenium Containing Inhibitor for the Treatment of Hepatocellular Cancer.

Pharmaceuticals (Basel, Switzerland)

Tagaram HR,Desai D,Li G,Liu D,Rountree CB,Gowda K,Berg A,Amin S,Staveley-O'Carroll KF,Kimchi ET

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 16-5988-85) in Immunohistochemistry

Thu Mar 24 00:00:00 EDT 2016

A Selenium Containing Inhibitor for the Treatment of Hepatocellular Cancer.

Pharmaceuticals (Basel, Switzerland)

Tagaram HR,Desai D,Li G,Liu D,Rountree CB,Gowda K,Berg A,Amin S,Staveley-O'Carroll KF,Kimchi ET

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 16-5988-85) in Immunohistochemistry

Thu Mar 24 00:00:00 EDT 2016

Vascular endothelial growth factor c/vascular endothelial growth factor receptor 3 signaling regulates chemokine gradients and lymphocyte migration from tissues to lymphatics.

Transplantation

Iwami D,Brinkman CC,Bromberg JS

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 16-5988-85) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Vascular endothelial growth factor c/vascular endothelial growth factor receptor 3 signaling regulates chemokine gradients and lymphocyte migration from tissues to lymphatics.

Transplantation

Iwami D,Brinkman CC,Bromberg JS

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 16-5988-85) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Fbxw7 controls angiogenesis by regulating endothelial Notch activity.

PloS one

Izumi N,Helker C,Ehling M,Behrens A,Herzog W,Adams RH

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 16-5988-85) in Immunofluorescence

Tue Apr 02 00:00:00 EDT 2013

Fbxw7 controls angiogenesis by regulating endothelial Notch activity.

PloS one

Izumi N,Helker C,Ehling M,Behrens A,Herzog W,Adams RH

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 16-5988-85) in Immunofluorescence

Tue Apr 02 00:00:00 EDT 2013

Kidins220/ARMS mediates the integration of the neurotrophin and VEGF pathways in the vascular and nervous systems.

Cell death and differentiation

Cesca F,Yabe A,Spencer-Dene B,Scholz-Starke J,Medrihan L,Maden CH,Gerhardt H,Orriss IR,Baldelli P,Al-Qatari M,Koltzenburg M,Adams RH,Benfenati F,Schiavo G

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 16-5988-85) in Western Blot

Wed Feb 01 00:00:00 EST 2012

Embryonic vascular endothelial cells are malleable to reprogramming via Prox1 to a lymphatic gene signature.

BMC developmental biology

Kim H,Nguyen VP,Petrova TV,Cruz M,Alitalo K,Dumont DJ

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 16-5988-85) in Immunohistochemistry

Mon Jun 28 00:00:00 EDT 2010

Embryonic vascular endothelial cells are malleable to reprogramming via Prox1 to a lymphatic gene signature.

BMC developmental biology

Kim H,Nguyen VP,Petrova TV,Cruz M,Alitalo K,Dumont DJ

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 16-5988-85) in Immunohistochemistry

Mon Jun 28 00:00:00 EDT 2010

Spreds are essential for embryonic lymphangiogenesis by regulating vascular endothelial growth factor receptor 3 signaling.

Molecular and cellular biology

Taniguchi K,Kohno R,Ayada T,Kato R,Ichiyama K,Morisada T,Oike Y,Yonemitsu Y,Maehara Y,Yoshimura A

16-5988 was used in Western Blotting to investigate the overlap in functions of Spred-1 and Spred-2, showing that they are essential for embryonic lymphangiogenesis by regulating VEGFR3 signalling.

Fri Jun 01 00:00:00 EDT 2007

Spreds are essential for embryonic lymphangiogenesis by regulating vascular endothelial growth factor receptor 3 signaling.

Molecular and cellular biology

Taniguchi K,Kohno R,Ayada T,Kato R,Ichiyama K,Morisada T,Oike Y,Yonemitsu Y,Maehara Y,Yoshimura A

16-5988 was used in Western Blotting to investigate the overlap in functions of Spred-1 and Spred-2, showing that they are essential for embryonic lymphangiogenesis by regulating VEGFR3 signalling.

Fri Jun 01 00:00:00 EDT 2007

Vascular endothelial growth factor-C and its receptor VEGFR-3 in the nasal mucosa and in nasopharyngeal tumors.

The American journal of pathology

Saaristo A,Partanen TA,Arola J,Jussila L,Hytönen M,Mäkitie A,Vento S,Kaipainen A,Malmberg H,Alitalo K

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 16-5988-85) in Immunohistochemistry

Sat Jul 01 00:00:00 EDT 2000

Vascular endothelial growth factor receptor-3 in lymphangiogenesis in wound healing.

The American journal of pathology

Paavonen K,Puolakkainen P,Jussila L,Jahkola T,Alitalo K

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 16-5988-85) in Immunohistochemistry

Mon May 01 00:00:00 EDT 2000

Bioinformatics

Protein Aliases: chylous ascites; FLT-4; Fms-like tyrosine kinase 4; receptor protein tyrosine kinase; soluble VEGFR 3; sVEGFR 3; Tyrosine-protein kinase receptor FLT4; Vascular endothelial growth factor receptor 3; vascular endothelial growth factor receptor-3; VEGF R3; VEGFR-3

Gene Aliases: AI323512; Chy; Flt-4; Flt4; VEGFR-3; VEGFR3

UniProt ID: (Mouse) P35917

Entrez Gene ID: (Mouse) 14257

Molecular Function: transmembrane signal receptor