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CD314 (NKG2D) Monoclonal Antibody (CX5), Functional Grade, eBioscience™
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FIGURE: 1 / 14
CD314 (NKG2D) Antibody (16-5882-81) in Flow
Nature communications 2024 -
Fig. 7 ZIKV infection of alpha-IFNAR-treated C57BL/6 mice promotes bystander activation independent of viral load. C57BL/6 mice were treated with alpha-IFNAR antibody at -1, 0, 1, 3, 5 dpi and infected via FTPD with 1.4 x 10 6 ZIKV PFU. Ifnar -/- mice were infected via FTPD with 4 x 10 5 ZIKV PFU. a Viral titer in the serum at 3 dpi (n =4, 5). b Spleen cells were isolated for flow cytometry at 8 dpi and CD4... View More
Product Details
16-5882-81
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
CX5
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: The CX5 monoclonal antibody reacts with the mouse NKG2D, a lectin-like molecule expressed on both human and mouse NK cells. Mouse NKG2D binds to retinoic acid-inducible RAE-1alpha, -beta, -gamma, -delta, -ε and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors, through the DAP12/DAP10 adaptor molecules. NKG2D is expressed by all spleen and liver NK cells, NK1.1^+ thymocytes, in vitro activated LAK cells, and a subset of splenic NKT cells.
Applications Reported:The CX5 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of binding of NKG2D to its ligands, RAE-1 and H60, and inhibition NKG2D-dependent NK cell-mediated cytotoxicity against NKG2D ligand-bearing tumors in vitro and in vivo .
Applications Tested: The CX5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that when using this antibody for staining the secondary must be Biotin anti-rat(Product # 12-5783-81). Expression has been confirmed on C57BL/6 and NWNA but not Balb/c or DBA/2 mice. Other mice strains have not been tested. No crossreactivity to Ly-49A, C, D or G2 has been observed. Addition of monoclonal antibody 3D10 to cell cultures induces lysis of target cells.
Applications Reported:This 3D10 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 3D10 antibody has been tested by flow cytometric analyis of C57Bl/6 splenocytes. This can be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer. Use this buffer whenever staining with NovaFluor conjugates, including single-color compensation controls using cells. Whenever possible, we recommend adding CellBlox Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100µL of cell sample containing 10^3 to 10^8 cells.
Excitation: 509 nm; Emission: 614 nm; Laser: 488 nm (Blue) Laser
NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more
Target Information
Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. NK cells can regulate specific humoral and cell-mediated immunity, and preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NK gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular C terminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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