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Invitrogen

IL-17A Polyclonal Antibody, Biotin, PeproTech®

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IL-17A Polyclonal Antibody, Biotin, PeproTech®

Product Details

500-P07GBT-500UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.25-1.0 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Goat

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human IL-17A
View immunogen

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Amount

500 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_3251038

Product Specific Information

AA Sequence of recombinant protein: MIVKAGITIP RNPGCPNSED KNFPRTVMVN LNIHNRNTNT NPKRSSDYYN RSTSPWNLHR NEDPERYPSV IWEAKCRHLG CINADGNVDY HMNSVPIQQE ILVLRREPPH CPNSFRLEKI LVSVGCTCVT PIVHHVA.

Preparation: Produced from sera of goats immunized with highly pure Recombinant Human IL-17A. Anti-Human IL-17A-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Human IL-17A by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-17A (500-P07G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-17A.

Western Blot: To detect hIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIL-17A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P07GBT-1MG will be provided as 2 x 500 µg

Target Information

Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; IL-17; ILN; inflammatory cytokine; Interleukin; interleukin 17; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin-17; Interleukin-17A; Interleukin17A; R-IL-17-A

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Gene Aliases: CTLA-8; CTLA8; IL-17; IL-17A; IL17; IL17A; ILA17

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UniProt ID: (Human) Q16552

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Entrez Gene ID: (Human) 3605

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Function(s)
cytokine activity protein binding protein homodimerization activity protein heterodimerization activity
Process(es)
positive regulation of antimicrobial peptide production adaptive immune response immune system process apoptotic process inflammatory response immune response Notch signaling pathway cell-cell signaling cell death response to wounding gene expression positive regulation of keratinocyte proliferation keratinocyte differentiation positive regulation of interleukin-1 beta production positive regulation of interleukin-12 production positive regulation of interleukin-16 production positive regulation of interleukin-23 production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production interleukin-17A-mediated signaling pathway keratinocyte proliferation innate immune response positive regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter defense response to Gram-negative bacterium defense response to Gram-positive bacterium defense response to fungus positive regulation of protein kinase B signaling intestinal epithelial structure maintenance cellular response to interleukin-1 fibroblast activation regulation of primary metabolic process interleukin-17-mediated signaling pathway granulocyte migration negative regulation of inflammatory response to wounding positive regulation of cytokine production involved in inflammatory response positive regulation of bicellular tight junction assembly positive regulation of tumor necrosis factor superfamily cytokine production negative regulation of interleukin-17-mediated signaling pathway positive regulation of chemokine (C-X-C motif) ligand 1 production
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