Search
Invitrogen
c-MAF Recombinant Mouse Monoclonal Antibody (sym0F1), Alexa Fluor™ Plus 488
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 3
c-MAF Antibody (740099MP488) in Flow
Flow cytometry analysis of c-MAF Recombinant Mouse Monoclonal Antibody (sym0F1), Alexa Fluor™ Plus 488 (Product # 740099MP488) was performed using RPMI 8226 and Daudi cells. Cells were fixed with eBioscience™ Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00), permeabilized and stained with 0.5 µg of the c-MAF Recombinant Mouse Monoclonal Antibody (sym0F1), Alexa Fluor™ Plus 488 (Product # 740099MP488). Higher expression of c-MAF was observed in RPMI 8226 (pink histogram) in comparison to Daudi (blue histogram). Viable c... View More
Product Details
740099MP488
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
sym0F1
Conjugate
Excitation/Emission Max
493/518 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Protein A/G
Storage buffer
proprietary buffer, pH 6.3-7.3
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 494 nm; Emission: 519 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
c-maf is the cellular counterpart of oncogenic v-maf that belongs to the family of basic region leucine zipper domain transcription factors. The leucine-zipper domain is involved in the interaction with LRPICD. There are two forms of human c-maf mRNA, c-maf-long and c-maf-short. It is identified in the genome of the acute transforming avian retrovirus AS42. c-maf targets are IL-4 in Th2 cells, the crystalline genes in lens fiber cells, insulin gene in islet, p53 and L7 where it exerts its transcriptional role through binding to a Maf recognition element (MARE). It regulates Th2 differentiation and lineage-specific hematopoiesis. c-maf is a transcription factor for IL-10 gene expression in LPS-activated macrophages. Chromosomal aberration involving maf is found in some forms of multiple myeloma. It is expressed in myeloma cell lines and resting monocytes/macrophages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene; b-zip transcription factor; bZIP Maf transcription factor; c-maf proto-oncogene; MGC71685; Proto-oncogene c-Maf; T lymphocyte c-maf long form; Transcription factor Maf; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog; V-maf musculoaponeurotic fibrosarcoma oncogene homolog
Gene Aliases: AYGRP; c-MAF; CCA4; CTRCT21; MAF
UniProt ID: (Human) O75444
Entrez Gene ID: (Human) 4094
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
identical protein binding
sequence-specific DNA binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
lens development in camera-type eye
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
response to nutrient
gene expression
positive regulation of gene expression
megakaryocyte differentiation
regulation of chondrocyte differentiation
positive regulation of transcription from RNA polymerase II promoter
cell development
inner ear development
lens fiber cell differentiation
integrated stress response signaling
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support