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GPNMB Recombinant Rat Monoclonal Antibody (CTSREVL), Alexa Fluor™ Plus 555
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FIGURE: 1 / 5
GPNMB Antibody (740113TP555) in ICC/IF
Immunofluorescence analysis of GPNMB was performed using GPNMB Recombinant Rat Monoclonal Antibody (CTSREVL), Alexa Fluor™ Plus 555 (Product # 740113TP555) on 70-80% confluent B16-F10 and EL4 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and then blocked with 2% BSA for 1 hour at room temperature. The cells were stained with GPNMB Recombinant Rat Monoclonal Antibody (CTSREVL), Alexa Fluor™ Plus 555 (Product # 740113TP555, 1:100) in 0.1% BSA, incubated at 4 degree Celsius overnight, Panel a) shows representative image ... View More
Product Details
740113TP555
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
CTSREVL
Conjugate
Excitation/Emission Max
558/572 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
proprietary buffer, pH 6.3-7.3
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DC-HIL; dendritic cell-associated heparin sulfate proteoglycan-dependent integrin ligand; Dendritic cell-associated transmembrane protein; iris pigment dispersion; Osteoactivin; Transmembrane glycoprotein NMB
Gene Aliases: DC-HIL; Dchil; Gpnmb; Hgfin; ipd; Nmb
UniProt ID: (Mouse) Q99P91
Entrez Gene ID: (Mouse) 93695
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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