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Proteintech
GAPDH Monoclonal Antibody (1E6D9), CoraLite® Plus 750
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
CL750-60004
Product Specifications
Species Reactivity
Human,
Mouse,
Plant,
Rat,
Yeast,
Zebrafish
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
1E6D9
Immunogen
GAPDH fusion protein Ag0766
Conjugate
CoraLite® Plus 750
CoraLite® Plus 750
CoraLite® Plus 750
Excitation/Emission Max
755/780 nm
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.5% BSA
Contains
0.05% ProClin 300
Storage conditions
-20°C, store in dark
Shipping conditions
Wet ice
Product Specific Information
Immunogen sequence: MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 38 kDa BFA-dependent ADP-ribosylation substrate; BARS-38; EC 1.2.1.12; GAPA; GAPA-1; GAPC; GAPC-1; GAPC1; GAPCP-1; GAPCp1; GAPDH; GAPDH-2; GAPDH-A; glyceraldehyde 3-phosphate dehydrogenase, testis-specific; Glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase 2; glyceraldehyde-3-phosphate dehydrogenase type 2; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; HGNC:4141; MGC88685; Peptidyl-cysteine S-nitrosylase GAPDH; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase
Gene Aliases: BARS-38; bb02e05; cb350; cb609; CDABP0047; fb71f08; fk58c09; g3pdh; GAPD; Gapd-s; GAPD2; GAPDH; gapdh-2; GAPDH2; GAPDHS; GAPDS; HSD-35; HSD35; mg:bb02e05; OK/SW-cl.12; wu:fb33a10; wu:fb71f08; wu:fk58c09; wu:ft80f05; zgc:76908
UniProt ID: (Mouse) P16858, (Rat) P04797, (Rat) Q9ESV6, (Mouse) Q64467, (Zebrafish) Q5XJ10, (Zebrafish) Q5MJ86
Entrez Gene ID: (Mouse) 14433, (Rat) 24383, (Rat) 66020, (Mouse) 14447, (Zebrafish) 317743, (Zebrafish) 406367
glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
microtubule binding
peptidyl-cysteine S-nitrosylase activity
identical protein binding
protein binding
NADP binding
NAD binding
molecular_function
oxidoreductase activity
oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
transferase activity
nucleotide binding
dehydrogenase
microtubule cytoskeleton organization
carbohydrate metabolic process
gluconeogenesis
glycolytic process
apoptotic process
multicellular organism development
negative regulation of translation
peptidyl-cysteine S-trans-nitrosylation
protein stabilization
neuron apoptotic process
oxidation-reduction process
response to ammonium ion
cellular response to interferon-gamma
regulation of translation
glucose metabolic process
spermatid development
sperm motility
positive regulation of glycolytic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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