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Proteintech
GAPDH Monoclonal Antibody (1E6D9), HRP
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Product Details
HRP-60004
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Plant,
Rat,
Zebrafish
Published species
Hamster,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
1E6D9
Immunogen
Recombinant protein
Conjugate
Form
Liquid
Concentration
0.67 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.5% BSA
Contains
0.02% thimerosal, 0.05% ProClin 300
Storage conditions
-20°C, store in dark
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; BARS-38; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; GAPDH; GAPDH-2; glyceraldehyde 3-phosphate dehydrogenase, testis-specific; Glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase 2; glyceraldehyde-3-phosphate dehydrogenase type 2; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; HGNC:4141; MGC88685; Peptidyl-cysteine S-nitrosylase GAPDH; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase
Gene Aliases: BARS-38; bb02e05; cb350; cb609; CDABP0047; fb71f08; fk58c09; G3PD; g3pdh; GAPD; Gapd-s; GAPD2; GAPDH; GAPDH-2; GAPDH2; GAPDHS; GAPDS; HEL-S-162eP; HEL-S-278; HSD-35; HSD35; mg:bb02e05; OK/SW-cl.12; wu:fb33a10; wu:fb71f08; wu:fk58c09; wu:ft80f05; zgc:76908
UniProt ID: (Human) P04406, (Human) O14556, (Mouse) P16858, (Rat) P04797, (Rat) Q9ESV6, (Mouse) Q64467, (Zebrafish) Q5XJ10, (Zebrafish) Q5MJ86
Entrez Gene ID: (Human) 2597, (Human) 26330, (Mouse) 14433, (Rat) 24383, (Rat) 66020, (Mouse) 14447, (Zebrafish) 317743, (Zebrafish) 406367
glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
protein binding
microtubule binding
peptidyl-cysteine S-nitrosylase activity
identical protein binding
NADP binding
NAD binding
molecular_function
oxidoreductase activity
oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
transferase activity
nucleotide binding
dehydrogenase
microtubule cytoskeleton organization
gluconeogenesis
regulation of macroautophagy
negative regulation of translation
peptidyl-cysteine S-trans-nitrosylation
protein stabilization
neuron apoptotic process
canonical glycolysis
cellular response to interferon-gamma
spermatid development
sperm motility
positive regulation of glycolytic process
carbohydrate metabolic process
glycolytic process
apoptotic process
multicellular organism development
oxidation-reduction process
response to ammonium ion
regulation of translation
glucose metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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