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Bethyl Laboratories
UPF3B Polyclonal Antibody
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FIGURE: 1 / 1
UPF3B Antibody (A303-689A) in IP
Detection of human UPF3B by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-UPF3B antibody (Product # A303-689A) used for IP at 6 µg/mg lysate. UPF3B was also immunoprecipitated by rabbit anti-UPF3B antibody A303-688A, which recognizes an upstream epitope. For blotting immunoprecipitated UPF3B, A303-688A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
Product Details
A303-689A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 433 and 483 of Human Up-Frameshift Suppressor 3 Regulator of Nonsense Transcripts Homolog B.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
UPF3B is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The protein is one of two functional homologs to yeast Upf3p. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hUpf3B; hUpf3p-X; Nonsense mRNA reducing factor 3B; Regulator of nonsense transcripts 3B; unnamed protein product; Up-frameshift suppressor 3 homolog B; Up-frameshift suppressor 3 homolog on chromosome X; UPF3 regulator of nonsense transcripts homolog B
Gene Aliases: HUPF3B; MRX62; MRX82; MRXS14; RENT3B; UPF3B; UPF3BP1; UPF3BP2; UPF3BP3; Upf3p-X; UPF3X
UniProt ID: (Human) Q9BZI7
Entrez Gene ID: (Human) 65109
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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