For researchers working with human and mouse pluripotent stem cells (PSCs), reliable feeder-dependent culture systems are important for maintaining pluripotency, reproducibility, and scalability. The Gibco KnockOut Media family offers a solution—cited in thousands of peer-reviewed publications—for feeder-based PSC workflows. Built on defined formulations and rigorously tested performance, KnockOut products help enable robust PSC maintenance, differentiation, and derivation.

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Choose the Gibco KnockOut products that best fit your stem cell culture needs

ProductKnockOut Serum Replacement (KSR)

KnockOut DMEM

KnockOut DMEM/F-12

ApplicationFeeder-dependent PSC cultureFeeder-dependent PSC cultureNeural stem cell culture
Benefits
  • A defined substitute for serum, meant for the culture of ESCs and iPSCs
  • Cited in over 2,000 publications for various PSC-related applications
  • Helps to reduce the burden of qualifying reagents when transitioning to clinical applications
  • A chemically defined basal medium designed for ESCs and iPSCs
  • Mimics the low-osmolality environment of mouse embryonic tissues
  • Reduces PSC differentiation, especially when used with KnockOut SR – Multi-Species
  • A chemically defined, low-osmolality basal medium without HEPES buffer
  • Supports expansion and differentiation of neural stem cells
  • Maintains human ESCs and iPSCs with normal morphology and karyotype
CTS versionCTS KnockOut SR XenoFree MediumCTS KnockOut DMEMCTS KnockOut DMEM/F-12

Learn more about KnockOut Serum Replacement (KSR)

Learn more about CTS KnockOut SR XenoFree Medium

KnockOut media resources

Posters
Protocols

Publications
  1. Guo G, Pinello L, Han X, et al. (2016) Serum-based culture conditions provoke gene expression variability in mouse embryonic stem cells as revealed by single-cell analysis. Cell Reports 14(4):956–965. doi:10.1016/j.celrep.2015.12.089
  2. Sundberg M, Bogetofte H, Lawson T, et al. (2013) Improved cell therapy protocols for Parkinson's disease based on differentiation efficiency and safety of hESC-, hiPSC-, and non-human primate iPSC-derived dopaminergic neurons. Stem Cells 31(8):1548–1562. doi:10.1002/stem.141
  3. Liu J, Koscielska KA, Cao Z, et al. (2012) Signaling defects in iPSC-derived fragile X premutation neurons. Human Molecular Genetics 21(17):3795–3805. doi:10.1093/hmg/dds207
  4. Davies TJ, Fairchild PJ (2012) Optimization of protocols for derivation of mouse embryonic stem cell lines from refractory strains, including the non-obese diabetic mouse. Stem Cells and Development 21(10):1688–1700. doi:10.1089/scd.2011.042
  5. Ef Hirai H, Katoku-Kikyo N, Karian P, et al. (2012) Efficient iPS cell production with the MyoD transactivation domain in serum-free culture. PLoS One 7(3):e34149. doi:10.1371/journal.pone.003414
  6. Wang A, Tang Z, Park IH, et al. (2011) Induced pluripotent stem cells for neural tissue engineering. Biomaterials 32(22):5023–5032. doi:10.1016/j.biomaterials.2011.03.070
  7. Hamanaka S, Yamaguchi T, Kobayashi T, et al. (2011) Generation of germline-competent rat induced pluripotent stem cells. PLoS One 6(7):e22008. doi:10.1371/journal.pone.0022008
  8. Koh KP, Yabuuchi A, Rao S, et al. (2011) Tet1 and Tet2 regulate 5-hydroxymethylcytosine production and cell lineage specification in mouse embryonic stem cells. Cell Stem Cell 8(2):200–213. doi:10.1016/j.stem.2011.01.008
  9. Li Y, Cang M, Lee AS, et al. (2011) Reprogramming of sheep fibroblasts into pluripotency under a drug-inducible expression of mouse-derived defined factors. PLoS One 6(1):e15947. doi:10.1371/journal.pone.0015947
  10. Zhang N, An MC, Montoro D, et al. (2010) Characterization of human Huntington's disease cell model from induced pluripotent stem cells. PLoS Currents 2:RRN1193. doi:10.1371/currents.RRN1193
  11. Li L, Fukunaga-Kalabis M, Yu H, et al. (2010) Human dermal stem cells differentiate into functional epidermal melanocytes. Journal of Cell Science 123(Pt 6):853–860. doi:10.1242/jcs.061598
  12. Zhao XY, Li W, Lv Z, et al.(2010) Efficient and rapid generation of induced pluripotent stem cells using an alternative culture medium. Cell Res 20(3):383–386. doi: 10.1038/cr.2010.26
  13. Li W, Ding S (2010) Generation of novel rat and human pluripotent stem cells by reprogramming and chemical approaches. Methods Mol Biol 636:293–300. doi: 10.1007/978-1-60761-691-7_18

For Research Use Only. Not for use in diagnostic procedures.

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