CTS™ KnockOut™ DMEM
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Gibco™

CTS™ KnockOut™ DMEM

CTS KnockOut D-MEM is a basal medium optimized for the growth of undifferentiated human embryonic (hESCs) and induced pluripotent stem cells (hiPSCs).
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Catalog NumberQuantity
A1286101500 mL
Catalog number A1286101
Price (USD)
61.75
Each
Add to cart
Quantity:
500 mL
Price (USD)
61.75
Each
Add to cart

Gibco CTS KnockOut D-MEM is a basal medium that supports the growth of undifferentiated human embryonic (hESCs) and induced pluripotent stem cells (hiPSCs) and contains no L-glutamine. The osmolarity is optimized to approximate that of mouse embryonic tissue.

  • Defined D-MEM for hESC/iPSC stem cell culture
  • Osmolarity optimized for growth of undifferentiated hESC and iPSC
  • Reduces the burden in qualifying reagents during your transition from research to clinical applications. Gibco CTS products are of high quality and are supplied with harmonized documentation such as Certificates of Analysis, Certificates of Origin, and access to authorization letters for our Drug Master Files, as appropriate.
Specifications
Cell TypeEmbryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)
For Use With (Application)Cell and Gene Therapy Research, Development, and Manufacturing
FormatBottle
Manufacturing QualityISO 13485, MDSAP, FDA-registered, 21 CFR 820
Product TypeCell Culture Medium
Quantity500 mL
Shelf Life12 months from date of manufacture
Shipping ConditionAmbient
ClassificationAnimal Origin-free
FormLiquid
SterilitySterile-filtered
Unit SizeEach
Contents & Storage
Store at 2–8°C and protect from light.

Frequently asked questions (FAQs)

What is CTS?

The Gibco Cell Therapy Systems (CTS) portfolio of cell and gene therapy products are GMP manufactured, safety tested, and backed by regulatory documentation to support your transition from discovery through clinical and commercial manufacturing. Through our CTS solutions, we are committed to helping customers streamline therapeutic development, minimize risk, and ease the burden on their quality systems. Learn more here.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I characterize human embryonic stem ( ES) cells?

Human ES cells are generally characterized by their typical morphology (they grow as tightly packed clusters of small cells with high ratio of nucleus to cytoplasm); surface marker expression; RT-PCR detection of stem cell-specific gene expression (such as Oct3/4, Sox2, and Nanog); alkaline phosphatase staining, and telomerase activity assay. The most commonly used ES specific surface markers include stage-specific embryonic antigens SSEA-3 and SSEA-4 for human ES cells. Other ES-specific surface antigens also include TRA-1-60 and TRA-1-81. (Science 282:1145 (1998).

How are human embryonic stem (ES) cells derived?

Human ES cells are derived from human blastocyst inner cell masses, isolated by immunosurgery with rabbit antiserum to BeWO cells (a human trophoblast cell line) (Science 282:1145 (1998)).

What are ES cells?

Embryonic stem (ES) cells are derived from the early mammalian embryo and are capable of unlimited, undifferentiated proliferation in vitro while maintaining their potential to differentiate into a wide of range of adult tissues including germ cells. The pluripotency of the ES cells is normally demonstrated in vitro by inducing ES cells to differentiate into embryoid bodies and checking lineage-specific markers for differentiated cells in three body layers (endo, meso, and ectoderm), or injecting them into immunodeficient mice and determining the cell types produced in the teratomas.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

View all CTS™ KnockOut™ DMEM FAQs