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The new One Lambda™ AllType™ FASTPlex™ NGS HLA assay for Illumina™ and Ion GeneStudio™ systems has several advantages over its previous version , including an optimized primer mix for 11 HLA loci, more robust PCR amplification, greater on-target accuracy, an improved DRB primer design, and better locus balance. Exon 1 can be included in the multiplex assay to improve HLA allele resolution. Only one tube is needed to pool 96 samples for NGS library preparation since each sample is given a unique barcode, and only one universal adapter is required. The assay takes less than seven hours to complete and less than 90 minutes of hands-on time.
Senior Technical Manager, HLA and MMDx | Thermo Fisher Scientific
West Hills, CA USA
Nov 3, 2021
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