Learning Center Resources

Advancements in AllType FASTPlex 2021 International Clinical Webinar

The new One Lambda AllType FASTPlex NGS HLA assay for Illumina and Ion GeneStudio systems has several advantages over its previous version , including an optimized primer mix for 11 HLA loci, more robust PCR amplification, greater on-target accuracy, an improved DRB primer design, and better locus balance. Exon 1 can be included in the multiplex assay to improve HLA allele resolution. Only one tube is needed to pool 96 samples for NGS library preparation since each sample is given a unique barcode, and only one universal adapter is required. The assay takes less than seven hours to complete and less than 90 minutes of hands-on time.