HLA Typing by Next-Generation Sequencing (NGS) Using the AllType Fastplex Kit and the Relevance of Anti-HLA Antibody Monitoring After Kidney Transplantation 2021 International Clinical Webinar

Dr. Andreani discusses his laboratory’s evaluation of One Lambda AllType FASTPlex NGS 11 Loci Kit on the Ion Torrent and Illumina MiSeq NGS platforms showing 100% concordance on 240 samples previously typed using AllType NGS and demonstrates the changes and improvements to the assay.

HLA mismatch permissibility can be assessed by typing organ donors and sensitized recipients at high resolution and carefully analyzing the distinct molecular configurations of their HLA epitopes using with tools similar the HLAMatchmaker algorithm, which can also be accessed within the HLA Fusion software.  This makes it possible to determine a patient’s eplet load, which has been shown to be predictive of de novo DSA production after renal transplantation2. Dr. Andreani’s laboratory is using One Lambda LABScreen Single Antigen and supplemental LABScreen Single Antigen ExPlex beads in conjunction with HLAMatchmaker to identify these HLA epitope mismatches.

Speaker:
Marco Andreani, PhD
Laboratorio d’Immunogenetica dei Trapianti, Polo di Ricerca di San Paolo; Dipartimento di Oncoematologia e Terapia Cellulare e Genica; IRCCS Ospedale Pediatrico Bambino Gesù
Rome, Italy

Recorded:
Nov 17, 2021

References
  1. Tambur, A.R. and Claas, F.H.J. (2015), HLA Epitopes as Viewed by Antibodies: What Is it All About?. American Journal of Transplantation, 15: 1148-1154. https://doi.org/10.1111/ajt.13192.
  2. Lachmann, N. et al. Donor-Recipient Matching Based on Predicted Indirectly Recognizable HLA Epitopes Independently Predicts the Incidence of De Novo Donor-Specific HLA Antibodies Following Renal Transplantation. Am J Transplant. 2017 Dec;17(12):3076-3086. https://doi.org/10.1111/ajt.14393.