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Invitrogen

CD32b Monoclonal Antibody (AT130-2), APC, eBioscience™

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View all (32) CD32b (FCGR2B) antibodies

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Additional Information:

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Product Details

17-0321-80

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.5 µg/test
View 7 publications 7 publications
Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

AT130-2

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Amount

25 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573141

Product Specific Information

Description: The monoclonal antibody AT130-2 reacts with mouse CD32b, also known as Fc-gamma receptor IIb. CD32b is the only inhibitory member of the Fc-gamma receptor family. Immune complex engagement of CD32b results in the phosphorylation of its intracellular ITIM motif. This engagement serves to attenuate B cell receptor (BCR) signaling and other activating signals, reduces proliferation, reduces antibody production, and may also deliver apoptotic signals. CD32b is the only Fc-gamma receptor expressed by B cells. CD32b is also expressed on monocytes, dendritic cells, neutrophils, eosinophils and basophils, as well as B cell progenitors and plasma cells.

AT130-2 has been reported to have agonist activity.

Applications Reported: This AT130-2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AT130-2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: CD32 receptor 2; CD32 receptor 2a; CD32 receptor 2b; CD32A; fc gamma receptor IIB; Fc gamma receptor precursor; Fc gamma RIIB; fc-gamma RII; fc-gamma RII-a; Fc-gamma-1/gamma-2bR receptor precursor; Fc-gamma-R-beta-1 precursor; Fc-gamma-R-beta-2 precursor; Fc-gamma-RII; fc-gamma-RIIa; fc-gamma-RIIB; FcgammaRIIB; FcGR; FCGR2A1; fcRII-a; FGFR2B; igG Fc receptor II beta; low affinity immunoglobulin gamma Fc region receptor II; lymphocyte antigen 17; unnamed protein product

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Gene Aliases: CD32; F630109E10Rik; Fc[g]RII; Fcgr2; Fcgr2a; FcgRII; Fcr-2; Fcr-3; fcRII; Ly-17; Ly-m20; LyM-1

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UniProt ID: (Mouse) P08101

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Entrez Gene ID: (Mouse) 14130

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Function(s)
beta-amyloid binding protein binding IgG receptor activity low-affinity IgG receptor activity IgG binding immunoglobulin binding macromolecular complex binding
Process(es)
antibody-dependent cellular cytotoxicity negative regulation of type I hypersensitivity immune complex clearance negative regulation of immunoglobulin production negative regulation of inflammatory response to antigenic stimulus negative regulation of acute inflammatory response to antigenic stimulus negative regulation of humoral immune response mediated by circulating immunoglobulin receptor-mediated endocytosis phagocytosis, engulfment defense response cell surface receptor signaling pathway response to bacterium immunoglobulin mediated immune response dendrite development antigen processing and presentation of exogenous peptide antigen via MHC class II cerebellum development negative regulation of B cell proliferation endocytic recycling negative regulation of interleukin-10 production positive regulation of tumor necrosis factor production negative regulation of neutrophil apoptotic process Fc-gamma receptor signaling pathway positive regulation of JNK cascade regulation of long-term neuronal synaptic plasticity negative regulation of phagocytosis positive regulation of phagocytosis regulation of immune response negative regulation of immune response cellular response to molecule of bacterial origin Fc receptor-mediated immune complex endocytosis regulation of dendritic spine maintenance regulation of endoplasmic reticulum stress-induced neuron intrinsic apoptotic signaling pathway cellular response to beta-amyloid positive regulation of response to endoplasmic reticulum stress
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