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BCL6 Monoclonal Antibody (BCL-UP), APC, eBioscience™
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FIGURE: 1 / 1
BCL6 Antibody (17-9880-41) in Flow
Surface staining of human tonsil cells with Anti-Human CD4 eFluor® 450, CD19 PE, CD279 (PD-1) PE-Cy7, CD278 (ICOS) PerCP-eFluor® 710, and CD45RO FITC antibodies followed by intracellular staining with Anti-Human Bcl-6 APC antibody using the Foxp3 Fixation/Permeabilization Buffers (Product # 00-5521-00) and protocol. The histogram (right) demonstrates Bcl-6 staining of CD4+CD45RO+CD278highCD279high (purple histogram) and CD4+CD45RO+CD278lowCD279low (blue histogram) cells. Cell populations were gated as indicated on the contour plot (left). ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
17-9880-41
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
BCL-UP
Conjugate
APC
APC
APC
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody BCL-UP recognizes Bcl-6, also known as B-cell lymphoma 6,which is a member of the POZ family of transcriptional repressors. Bcl-6 is expressed most abundantly in germinal center B cells, as well as in differentiating and activated B cells. This 79 kDa protein is essential for germinal center B cell proliferation and survival. During B cell development, Bcl-6 plays an important role in promoting pre-B cell survival to enable the generation of a diverse B cell repertoire. Moreover, Bcl-6 is expressed constitutively in diffuse large B cell lymphomas (DLBCLs), Burkitt's lymphoma, and non-Hodgkin's lymphoma. Reports also suggest expression of Bcl-6 in CD4+ T follicular helper cells and memory T cells. Bcl-6 has been reported to be antagonized by Blimp-1.
Applications Reported: This BCL-UP antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This BCL-UP antibody has been pre-titrated and tested in human tonsil cells by intracellular staining with the Foxp3 Fixation/Permeabilization Buffers (Product # 00-5521-00) and protocol. Please see BestProtocols® Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Bcl-6 proto-oncogene product (a Kruppel-type zinc-finger protein) is mainly expressed in normal germinal center B cells and related lymphomas. Bcl-6 is involved in chromosome rearrangements at 3q27 in non-Hodgkin's lymphomas and Bcl-6 rearrangements have been detected in 33%-45% of diffuse large B cell lymphomas. Bcl-6 has been detected immunohistochemically in follicular lymphomas, diffuse large B cell lymphomas, Burkitt's lymphomas and in nodular, lymphocyte predominant Hodgkin's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B cell CLL/lymphoma 6; B-cell lymphoma 5 protein; B-cell lymphoma 5 protein (BCL5); B-cell lymphoma 6 protein transcript; BCL-5; BCL-6; cys his2 zinc finger transcription factor; cys-his2 zinc finger transcription factor; LAZ-3 protein; Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3); lymphoma-associated zinc finger gene on chromosome 3; protein LAZ-3; transcriptional repressor; B-cell CLL/lymphoma 6 spliced short isoform; transcriptional repressor; zinc finger protein 51; unnamed protein product; zinc finger and BTB domain-containing protein 27; Zinc finger and BTB domain-containing protein 27 (ZBTB27); zinc finger protein 51; Zinc Finger Protein 51 (ZNF51); zinc finger transcription factor BCL6S
Gene Aliases: BCL5; BCL6A; LAZ3; ZBTB27; ZNF51
UniProt ID: (Human) P41182
Entrez Gene ID: (Human) 604
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
intronic transcription regulatory region sequence-specific DNA binding
transcription corepressor binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
chromatin DNA binding
identical protein binding
sequence-specific DNA binding
DNA-binding transcription factor binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
regulation of cytokine production
regulation of germinal center formation
regulation of immune system process
negative regulation of B cell apoptotic process
cellular response to DNA damage stimulus
negative regulation of cell growth
type 2 immune response
regulation of cell proliferation
regulation of apoptotic process
positive regulation of apoptotic process
regulation of T cell differentiation
positive regulation of regulatory T cell differentiation
regulation of cell differentiation
positive regulation of cell differentiation
negative regulation of transcription, DNA-templated
regulation of inflammatory response
regulation of immune response
positive regulation of lymphocyte activation
regulation of leukocyte cell-cell adhesion
negative regulation of mitotic cell cycle DNA replication
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