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BCL6 Monoclonal Antibody (BCL-UP), PerCP-eFluor™ 710, eBioscience™
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FIGURE: 1 / 1
BCL6 Antibody (46-9880-42) in Flow
Surface staining of human tonsil cells with the Anti-Human CD4 eFluor® 450, CD19 APC-eFluor® 780, CD279 (PD-1) PE, CD278 (ICOS) PE-Cy7, and CD45RO FITC antibodies followed by intracellularstaining with Anti-Human Bcl-6 PerCP-eFluor® 710 antibody using the Foxp3 Fixation/Permeabilization Buffers (Product # 00-5521-00) and protocol. The histogram (right) demonstrates Bcl-6 staining of CD4+CD45RO+CD278highCD279high (purple histogram) and CD4+CD45RO+CD278lowCD279low (blue histogram) cells. Cell populations were gated as indicated on the contou... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
46-9880-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
BCL-UP
Conjugate
PerCP-eFluor™ 710
PerCP-eFluor™ 710
PerCP-eFluor™ 710
Excitation/Emission Max
482/708 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody BCL-UP recognizes Bcl-6, also known as B-cell lymphoma 6,which is a member of the POZ family of transcriptional repressors. Bcl-6 is expressed most abundantly in germinal center B cells, as well as in differentiating and activated B cells. This 79 kDa protein is essential for germinal center B cell proliferation and survival. During B cell development, Bcl-6 plays an important role in promoting pre-B cell survival to enable the generation of a diverse B cell repertoire. Moreover, Bcl-6 is expressed constitutively in diffuse large B cell lymphomas (DLBCLs), Burkitt's lymphoma, and non-Hodgkin's lymphoma. Reports also suggest expression of Bcl-6 in CD4+ T follicular helper cells and memory T cells. Bcl-6 has been reported to be antagonized by Blimp-1.
Applications Reported: This BCL-UP antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This BCL-UP antibody has been pre-titrated and tested by intracellular flow cytometric analysis of human tonsil cells using the Foxp3 Fixation/Permeabilization Buffers (Product # 00-5521-00) and protocol. Please see BestProtocols® Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.
Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Bcl-6 proto-oncogene product (a Kruppel-type zinc-finger protein) is mainly expressed in normal germinal center B cells and related lymphomas. Bcl-6 is involved in chromosome rearrangements at 3q27 in non-Hodgkin's lymphomas and Bcl-6 rearrangements have been detected in 33%-45% of diffuse large B cell lymphomas. Bcl-6 has been detected immunohistochemically in follicular lymphomas, diffuse large B cell lymphomas, Burkitt's lymphomas and in nodular, lymphocyte predominant Hodgkin's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: B cell CLL/lymphoma 6; B-cell lymphoma 5 protein; B-cell lymphoma 5 protein (BCL5); B-cell lymphoma 6 protein transcript; BCL-5; BCL-6; cys his2 zinc finger transcription factor; cys-his2 zinc finger transcription factor; LAZ-3 protein; Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3); lymphoma-associated zinc finger gene on chromosome 3; protein LAZ-3; transcriptional repressor; B-cell CLL/lymphoma 6 spliced short isoform; transcriptional repressor; zinc finger protein 51; unnamed protein product; zinc finger and BTB domain-containing protein 27; Zinc finger and BTB domain-containing protein 27 (ZBTB27); zinc finger protein 51; Zinc Finger Protein 51 (ZNF51); zinc finger transcription factor BCL6S
Gene Aliases: BCL5; BCL6A; LAZ3; ZBTB27; ZNF51
UniProt ID: (Human) P41182
Entrez Gene ID: (Human) 604
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
intronic transcription regulatory region sequence-specific DNA binding
transcription corepressor binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
chromatin DNA binding
identical protein binding
sequence-specific DNA binding
DNA-binding transcription factor binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
regulation of cytokine production
regulation of germinal center formation
regulation of immune system process
negative regulation of B cell apoptotic process
cellular response to DNA damage stimulus
negative regulation of cell growth
type 2 immune response
regulation of cell proliferation
regulation of apoptotic process
positive regulation of apoptotic process
regulation of T cell differentiation
positive regulation of regulatory T cell differentiation
regulation of cell differentiation
positive regulation of cell differentiation
negative regulation of transcription, DNA-templated
regulation of inflammatory response
regulation of immune response
positive regulation of lymphocyte activation
regulation of leukocyte cell-cell adhesion
negative regulation of mitotic cell cycle DNA replication
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