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Invitrogen

BCL6 Monoclonal Antibody (BCL-UP), PerCP-eFluor™ 710, eBioscience™

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Product Details

46-9880-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.03 µg)/test
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Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

BCL-UP

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11063697

Product Specific Information

Description: The monoclonal antibody BCL-UP recognizes Bcl-6, also known as B-cell lymphoma 6,which is a member of the POZ family of transcriptional repressors. Bcl-6 is expressed most abundantly in germinal center B cells, as well as in differentiating and activated B cells. This 79 kDa protein is essential for germinal center B cell proliferation and survival. During B cell development, Bcl-6 plays an important role in promoting pre-B cell survival to enable the generation of a diverse B cell repertoire. Moreover, Bcl-6 is expressed constitutively in diffuse large B cell lymphomas (DLBCLs), Burkitt's lymphoma, and non-Hodgkin's lymphoma. Reports also suggest expression of Bcl-6 in CD4+ T follicular helper cells and memory T cells. Bcl-6 has been reported to be antagonized by Blimp-1.

Applications Reported: This BCL-UP antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This BCL-UP antibody has been pre-titrated and tested by intracellular flow cytometric analysis of human tonsil cells using the Foxp3 Fixation/Permeabilization Buffers (Product # 00-5521-00) and protocol. Please see BestProtocols® Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Bcl-6 proto-oncogene product (a Kruppel-type zinc-finger protein) is mainly expressed in normal germinal center B cells and related lymphomas. Bcl-6 is involved in chromosome rearrangements at 3q27 in non-Hodgkin's lymphomas and Bcl-6 rearrangements have been detected in 33%-45% of diffuse large B cell lymphomas. Bcl-6 has been detected immunohistochemically in follicular lymphomas, diffuse large B cell lymphomas, Burkitt's lymphomas and in nodular, lymphocyte predominant Hodgkin's disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: B cell CLL/lymphoma 6; B-cell lymphoma 5 protein; B-cell lymphoma 5 protein (BCL5); B-cell lymphoma 6 protein; B-cell lymphoma 6 protein transcript; BCL-5; BCL-6; cys his2 zinc finger transcription factor; cys-his2 zinc finger transcription factor; LAZ-3 protein; Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3); lymphoma-associated zinc finger gene on chromosome 3; Protein LAZ-3; transcriptional repressor; B-cell CLL/lymphoma 6 spliced short isoform; transcriptional repressor; zinc finger protein 51; unnamed protein product; Zinc finger and BTB domain-containing protein 27; Zinc finger and BTB domain-containing protein 27 (ZBTB27); Zinc finger protein 51; Zinc Finger Protein 51 (ZNF51); zinc finger transcription factor BCL6S

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Gene Aliases: BCL5; BCL6; BCL6A; LAZ3; ZBTB27; ZNF51

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UniProt ID: (Human) P41182

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Entrez Gene ID: (Human) 604

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Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding intronic transcription regulatory region sequence-specific DNA binding transcription corepressor binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding zinc ion binding chromatin DNA binding identical protein binding sequence-specific DNA binding metal ion binding DNA-binding transcription factor binding sequence-specific double-stranded DNA binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter cell morphogenesis regulation of cytokine production negative regulation of cell-matrix adhesion plasma cell differentiation immune system process germinal center formation regulation of germinal center formation regulation of immune system process negative regulation of type 2 immune response negative regulation of B cell apoptotic process regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter inflammatory response cellular response to DNA damage stimulus cell-matrix adhesion Rho protein signal transduction spermatogenesis protein localization cell proliferation pyramidal neuron differentiation actin cytoskeleton organization B cell differentiation negative regulation of cell growth positive regulation of B cell proliferation heterochromatin assembly negative regulation of mast cell cytokine production mononuclear cell proliferation negative regulation of mononuclear cell proliferation negative regulation of Rho protein signal transduction type 2 immune response B cell proliferation regulation of cell proliferation regulation of T cell proliferation regulation of apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process regulation of memory T cell differentiation T-helper 2 cell differentiation regulation of T cell differentiation positive regulation of regulatory T cell differentiation regulation of cell differentiation positive regulation of cell differentiation negative regulation of T-helper 2 cell differentiation positive regulation of neuron differentiation negative regulation of Notch signaling pathway negative regulation of transcription, DNA-templated isotype switching to IgE isotypes negative regulation of isotype switching to IgE isotypes erythrocyte development cell motility regulation of inflammatory response regulation of immune response positive regulation of lymphocyte activation negative regulation of plasma cell differentiation regulation of leukocyte cell-cell adhesion negative regulation of mitotic cell cycle DNA replication positive regulation of cell motility negative regulation of cellular senescence
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