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Invitrogen

CD115 (c-fms) Monoclonal Antibody (AFS98), PE-Cyanine7, eBioscience™

28 Published Figures
37 References
View all (93) CSF1R (CD115) antibodies

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Product Details

25-1152-80

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.125 µg/test
View 37 publications 37 publications
Product Specifications

Species Reactivity

Mouse

Published species

Fish, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

AFS98

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Amount

25 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573385

Product Specific Information

Description: The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.

Applications Reported: This AFS98 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AFS98 antibody has been tested by flow cytometric analysis of mouse BM derived macrophages. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CSF1R (FMS) is involved in the production, differentiation, and function of macrophages, and is implicated in promyelocytic leukemias. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. CSF1R is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and plays an important role in innate immunity and in inflammatory processes. The first intron of the CSF1R gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: c-fms; CSF-1 receptor; CSF-1-R; CSFIR; EC 2.7.10.1; kinase CSFR; OTTHUMP00000224093; proto-oncogene c-Fms; proto-oncogene fms; unnamed protein product

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Gene Aliases: CD115; CSF-1R; Csfmr; Fim-2; Fim2; Fms; M-CSF-R; M-CSFR

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UniProt ID: (Mouse) P09581

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Entrez Gene ID: (Mouse) 12978

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Function(s)
protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity macrophage colony-stimulating factor receptor activity protein binding ATP binding growth factor binding protein phosphatase binding cytokine binding protein homodimerization activity
Process(es)
microglial cell activation neutrophil homeostasis positive regulation of protein phosphorylation myeloid leukocyte differentiation response to ischemia response to stress signal transduction transmembrane receptor protein tyrosine kinase signaling pathway axon guidance cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation regulation of cell shape anatomical structure morphogenesis positive regulation of gene expression positive regulation of macrophage chemotaxis negative regulation of myotube differentiation microglia development cell migration peptidyl-tyrosine phosphorylation cytokine-mediated signaling pathway telencephalon development dentate gyrus development olfactory bulb development forebrain neuron differentiation regulation of cell morphogenesis hemopoiesis osteoclast differentiation positive regulation of cell migration positive regulation of bone mineralization negative regulation of epithelial cell differentiation ruffle organization positive regulation of chemokine production positive regulation of tumor necrosis factor production regulation of mononuclear cell proliferation regulation of actin cytoskeleton organization monocyte homeostasis cellular response to macrophage colony-stimulating factor stimulus macrophage colony-stimulating factor signaling pathway positive regulation of tyrosine phosphorylation of STAT protein negative regulation of apoptotic process regulation of MAPK cascade positive regulation by host of viral process regulation of bone resorption cell-cell junction maintenance positive regulation of osteoclast differentiation protein autophosphorylation positive regulation of lymphocyte proliferation positive regulation of protein kinase B signaling positive regulation of protein tyrosine kinase activity microglial cell proliferation positive regulation of astrocyte activation positive regulation of ERK1 and ERK2 cascade positive regulation of leukocyte proliferation cellular response to cytokine stimulus positive regulation of macrophage proliferation positive regulation of cell cycle G1/S phase transition negative regulation of platelet formation regulation of macrophage migration positive regulation of macrophage migration neuron projection extension positive regulation of cell motility positive regulation of myoblast proliferation
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