SuperSignal™ West Dura Extended Duration Substrate

Name: SuperSignal™ West Dura Extended Duration Substrate
SKU: 34075

Thermo Scientific SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal forRead more

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Catalog Number	Quantity
34075	100 mL
34076	200 mL
37071	20 mL

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Catalog number 34075

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100 mL

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Thermo Scientific SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

SuperSignal West Dura Extended Duration Substrate characteristics include:

Sensitivity: mid-femtogram
Stability: 24-hr working solution stability; 1-year kit stability at room temperature
Compatibility: nitrocellulose and PVDF membranes
Signal duration: 24 hours
Recommended primary antibody concentration: 1:1,000–1:50,000 dilution (0.02–1.0 μg/mL)
Recommended secondary antibody concentration: 1:50,000–1:250,000 dilution (4–20 ng/mL)

SuperSignal West Dura Extended Duration Substrate for HRP is optimized for high sensitivity and long signal duration, making it ideal for cooled CCD camera-based detection systems. Unlike substrates with signals that decline to barely detectable levels in 30–60 minutes, the signal produced with SuperSignal West Dura chemiluminescent substrate is stable for 24 hours, allowing multiple film or camera exposures.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Kit ContentsLuminol/Enhancer, 50mL, Stable Peroxide Buffer, 50mL

Recommended Antibody Concentrations2°: 1:50-250K (4-20ng/mL), 1°: 1:5K (0.02- 1μg/mL)

SensitivityMid-femtogram

Signal Duration24 hr.

Sufficient For12 Mini-Gel Blots, 1000 sq. cm of Membrane

Detection MethodChemiluminescence

For Use With (Application)Western Blot

FormLiquid

Membrane CompatibilityNitrocellulose, PVDF

Product LineSuperSignal

Product TypeSubstrate

Quantity100 mL

SubstrateHorseradish Peroxidase

Unit SizeEach

Contents & Storage

Sufficient For: 12 mini-gel blots; 1000 cm² of membrane
• Luminol/Enhancer, 50 mL
• Stable Peroxide Buffer, 50 mL

Store at room temperature.

Frequently asked questions (FAQs)

What types of membrane work best with SuperSignal West Dura Extended Duration Substrate?

Most researchers use nitrocellulose or polyvinyldiflouride (PVDF) membranes with SuperSignal West Dura Extended Duration Substrate. Both work well, although nitrocellulose seems to be better suited in some applications than PVDF. In addition, charge-modified nylon membrane performs well with this substrate. Please also see our guide for choosing western blot membranes (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/transfer-proteins-western-blot/membranes-transfer-buffers-western-blotting/membranes-western-blotting.html).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Will SuperSignal West Dura Extended Duration Substrate work with nucleic acid blots (Southern blotting, etc.)?

Yes. However, SuperSignal West Dura Extended Duration Substrate was optimized for use in Western blots and is generally not sensitive enough for most nucleic acid protocols. For Southern and Northern blotting, use our North2South Chemiluminescent Nucleic Acid Hybridization and Detection Kit (Cat. No. 17097), North2South Chemiluminescent Substrate for HRP (Cat. No. 17295), or the Chemiluminescent Nucleic Acid Detection Kit, if probing for biotinylated probes (Cat. No. 89880).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

I used the SuperSignal West Dura Extended Duration Substrate. Why is the entire film black or showing a reversed image?

The antibody concentration (primary and secondary) is too high. Use the antibody dilutions described in the product instructions. Most background will disappear when the proper blocking reagent and a HRP conjugate concentration are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Why do I see more bands with the SuperSignal West Dura Extended Duration Substrate than I've seen in the past with other substrates?

Because this substrate is more sensitive than other chemiluminescent substrates you might detect low-abundance proteins that were not visible before. When using a more sensitive substrate, more careful optimization of blocking buffer steps and antibody concentrations is required.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What protocol should I use to strip and reprobe a blot detected with SuperSignal West Dura Extended Duration Substrate?

See Tech Tip # 23: Strip and reprobe Western blots.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all SuperSignal™ West Dura Extended Duration Substrate, 100 mL FAQs