Thermo Scientific™

SuperSignal™ West Dura Extended Duration Substrate

Catalog number:  34075

Related applications:

Protein Electrophoresis & Western Blotting

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Description

Thermo Scientific SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate with stable light output for mid-femtogram level detection for western blot analysis.

SuperSignal West Dura Extended Duration Substrate characteristics include:
Sensitivity: mid-femtogram
Stability: 24-hr working solution stability; 1-year kit stability at room temperature
Compatibility: nitrocellulose and PVDF membranes
Signal duration: 24 hours
Recommended primary antibody concentration: 1:1,000–1:50,000 dilution (0.02–1.0 µg/mL)
Recommended secondary antibody concentration: 1:50,000–1:250,000 dilution (4–20 ng/mL)

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SuperSignal West Dura Extended Duration Substrate for HRP is optimized for high sensitivity and long signal duration, making it ideal for cooled CCD camera-based detection systems. Unlike substrates with signals that decline to barely detectable levels in 30–60 minutes, the signal produced with SuperSignal West Dura chemiluminescent substrate is stable for 24 hours, allowing multiple film or camera exposures.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line: SuperSignal™
Recommended Antibody Concentrations: 2°: 1:50-250K (4-20ng/mL), 1°: 1:5K (0.02- 1µg/mL)
Signal Duration: 24 hr
Substrate Properties: Chemical Substrate
Substrate Type: HRP (Horseradish Peroxidase) Substrate
Detection Method: Chemiluminescent
Kit Contents: Luminol/Enhancer, 50mL
Quantity: 100 mL Kit
Sensitivity: Mid-femtogram

Contents & storage

Sufficient For: 12 mini-gel blots; 1000 cm2 of membrane
• Luminol/Enhancer, 50 mL
• Stable Peroxide Buffer, 50 mL

Store at room temperature.