Thermo Scientific™

SuperSignal™ West Dura Extended Duration Substrate

Catalog number: 34075
Thermo Scientific™

SuperSignal™ West Dura Extended Duration Substrate

Catalog number: 34075
Catalog Number
34075
Unit Size
100 mL
Price (USD)
Price: 368.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
200 mL
Price (USD)
Price: 650.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
20 mL
Price (USD)
Price: 123.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
34075100 mL
Price: 368.00
Your Price:
-
34076200 mL
Price: 650.00
Your Price:
-
3707120 mL
Price: 123.00
Your Price:
-
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Thermo Scientific SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

SuperSignal West Dura Extended Duration Substrate characteristics include:
Sensitivity: mid-femtogram
Stability: 24-hr working solution stability; 1-year kit stability at room temperature
Compatibility: nitrocellulose and PVDF membranes
Signal duration: 24 hours
Recommended primary antibody concentration: 1:1,000–1:50,000 dilution (0.02–1.0 µg/mL)
Recommended secondary antibody concentration: 1:50,000–1:250,000 dilution (4–20 ng/mL)

Compare and view all western blot chemiluminescent substrates ›

SuperSignal West Dura Extended Duration Substrate for HRP is optimized for high sensitivity and long signal duration, making it ideal for cooled CCD camera-based detection systems. Unlike substrates with signals that decline to barely detectable levels in 30–60 minutes, the signal produced with SuperSignal West Dura chemiluminescent substrate is stable for 24 hours, allowing multiple film or camera exposures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Chemiluminescent
Form
Liquid
Membrane Compatibility
Nitrocellulose, PVDF
Product Line
SuperSignal™
Product Type
Substrate
Quantity
100 mL
Sensitivity
mid-femtogram level
Substrate
Horseradish Peroxidase
Substrate Properties
Chemical Substrate
Substrate Type
HRP Substrate
Sufficient For
12 Mini-Gel Blots, 1000 sq. cm of Membrane
Validated Application
Western Blotting
Kit Contents
Luminol/Enhancer, 50mL, Stable Peroxide Buffer, 50mL
Recommended Antibody Concentrations
2°: 1:50-250K (4-20ng/mL), 1°: 1:5K (0.02- 1µg/mL)
Signal Duration
24 hr

Contents & Storage

Sufficient For: 12 mini-gel blots; 1000 cm2 of membrane
• Luminol/Enhancer, 50 mL
• Stable Peroxide Buffer, 50 mL

Store at room temperature.