Thermo Scientific™

SuperSignal™ West Dura Extended Duration Substrate

Catalog number:  34076

 Related applications:

Protein Assays and Analysis

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Thermo Scientific SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) HRP substrate with stable light output for mid-femtogram-level detection with CCD-based imagers.

Features of SuperSignal West Dura Extended Duration Substrate:

ECL—enhanced chemiluminescent substrate with high sensitivity and long signal duration for horseradish peroxidase (HRP)
24-hour signal duration—light emission is stable for 10 times longer than with typical ECL substrates; acquire multiple exposures to obtain publication-quality blot images
Great sensitivity—provides detection of mid-femtogram levels of target proteins
High intensity—immediate, stable signal generation provides easy detection via film or cooled CCD imaging systems
Stable reagent—24-hour working solution stability; 1-year kit stability at room temperature
Less antibody usage—works best with dilute antibody concentrations:
• 20ng to 1.0 µg/mL primary antibody (1:1000 to 1:50,000 dilution from 1 mg/mL stock)
• 4 to 20 ng/mL secondary antibody (1:50,000 to 1:250,000 dilution from 1 mg/mL stock)

More Product Data
Improved Western blot detection using CCD digital imaging
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Chemiluminescent
Product Line: SuperSignal™
Product Size: 200 mL
Substrate Properties: Chemical Substrate
Substrate Type: HRP (Horseradish Peroxidase) Substrate

Contents & storage

Sufficient For: 25 mini-gel blots; 2000 cm2 of membrane
• Luminol/Enhancer, 100 mL
• Stable Peroxide Buffer, 100 mL

Store at room temperature.


Manuals & protocols