Thermo Scientific iodoTMTsixplex Label Reagents enable concurrent identification and multiplexed quantitation of proteins in different samples using tandem mass spectrometry. The iodoTMT™ Reagents are sets of isobaric (mass and structure) isomers that are iodoacetyl-activated for covalent, irreversible labeling of sulfhydryl (—SH) groups. Similar to iodoacetamide, iodoTMT Reagents react specifically with reduced cysteines (Cys) in peptides and proteins. IodoTMT Reagents can be differentiated by mass spectrometry (MS), enabling quantitation of the relative abundance of cysteine modifications, such as S-nitrosylation, oxidation and disulfide bonds, in cultured cells grown or treated with different conditions.
Features of iodoTMTsixplex Label Reagents:
•
Specific—only reacts with sulfhydryl groups (reduced, unmodified cysteine residues)
•
Irreversible—labeled proteins and peptides are not susceptible to reducing agents
•
Flexible—options for duplex isotopic (MS) or sixplex isobaric (MS/MS) quantitation
•
Versatile—can be used to study cysteine modifications (e.g. di-sulfide bridges, S-nitrosylation)
IodoTMT Products replace our previously offered CysTMT™ Reagents, which utilized a dithiopyridine reactive group to reversibly label cysteine sulfhydryls. For information about customized reagent sets, please contact our Large Volume and Custom Sales Department.
These products are subject to a limited use label license.
More Product Data•
Specific labeling, enrichment and quantitation of S-nitrosylated peptides using iodoTMT reagentsRelated ProductsiodoTMTsixplex™ Isobaric Mass Tag Labeling KitiodoTMTzero™ Label Reagent, 5 x 0.2 mgImmobilized Anti-TMT™ Antibody ResinTMT™ Elution Buffer
