iodoTMTsixplex™ Isobaric Label Reagent Set, 1 x 0.2 mg

Description

Thermo Scientific iodoTMTsixplex Label Reagents enable concurrent identification and multiplexed quantitation of proteins in different samples using tandem mass spectrometry. The iodoTMT™ Reagents are sets of isobaric (mass and structure) isomers that are iodoacetyl-activated for covalent, irreversible labeling of sulfhydryl (—SH) groups. Similar to iodoacetamide, iodoTMT Reagents react specifically with reduced cysteines (Cys) in peptides and proteins. IodoTMT Reagents can be differentiated by mass spectrometry (MS), enabling quantitation of the relative abundance of cysteine modifications, such as S-nitrosylation, oxidation and disulfide bonds, in cultured cells grown or treated with different conditions.

Features of iodoTMTsixplex Label Reagents:

• Specific—only reacts with sulfhydryl groups (reduced, unmodified cysteine residues)
• Irreversible—labeled proteins and peptides are not susceptible to reducing agents
• Flexible—options for duplex isotopic (MS) or sixplex isobaric (MS/MS) quantitation
• Versatile—can be used to study cysteine modifications (e.g. di-sulfide bridges, S-nitrosylation)

IodoTMT Products replace our previously offered CysTMT™ Reagents, which utilized a dithiopyridine reactive group to reversibly label cysteine sulfhydryls. For information about customized reagent sets, please contact our Large Volume and Custom Sales Department.

These products are subject to a limited use label license.

More Product Data
• Specific labeling, enrichment and quantitation of S-nitrosylated peptides using iodoTMT reagents

Related Products
iodoTMTsixplex™ Isobaric Mass Tag Labeling Kit
iodoTMTzero™ Label Reagent, 5 x 0.2 mg
Immobilized Anti-TMT™ Antibody Resin
TMT™ Elution Buffer

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Specifications

Label Type:	Tandem Mass Tag™ (TMT) Labels
Product Line:	iodoTMTsixplex™
Workflow Step:	Protein Labeling
Detection Method:	Mass Spectrometry
Final Product Type:	Protein
For Use With (Equipment):	Mass Spectrometer
Starting Material:	Cell Lysate
Description:	iodoTMTsixplex Label Reagent Set, 1 x 0.2mg

Contents & storage