The Thermo Scientific iodoTMTzero Label Reagent can be used as an isotopic 'light' duplex tag for MS-level protein quantitation. This tag, along with the iodoTMTsixplex reagents can enable the quantitation of protein expression changes in cell-based and tissue samples that may not be amenable to metabolic isotopic labeling strategies (e.g., SILAC). Similar to iodoacetamide, iodoTMT Reagents react specifically with reduced cysteines (Cys) in peptides and proteins. IodoTMT Reagents can be differentiated by mass spectrometry (MS), enabling quantitation of the relative abundance of cysteine modifications, such as S-nitrosylation, oxidation and disulfide bonds, in cultured cells grown or treated with different conditions.
Features of the iodoTMTzero Label Reagent:
—only reacts with sulfhydryl groups (reduced, unmodified cysteine residues)
—labeled proteins and peptides are not susceptible to reducing agents
—options for duplex isotopic (MS) or sixplex isobaric (MS/MS) quantitation
—can be used to study cysteine modifications (e.g. di-sulfide bridges, S-nitrosylation)
IodoTMT Products replace our previously offered CysTMT™ Reagents, which utilized a dithiopyridine reactive group to reversibly label cysteine sulfhydryls. For information about customized reagent sets, please contact our Large Volume and Custom Sales Department.
These products are subject to a limited use label license.More Product Data
• Specific labeling, enrichment and quantitation of S-nitrosylated peptides using iodoTMT reagentsRelated ProductsiodoTMTsixplex™ Label Reagent Set, 1 x 0.2 mgiodoTMTsixplex™ Isobaric Mass Tag Labeling KitImmobilized Anti-TMT™ Antibody ResinTMT™ Elution Buffer
For Research Use Only. Not for use in diagnostic procedures.