CyQUANT™ Cell Proliferation Assays
CyQUANT™ Cell Proliferation Assays
인용 및 참조 문헌 (7)
Invitrogen™

CyQUANT™ Cell Proliferation Assays

CyQUANT™ NF Cell Proliferation Assay Kit는 빠르고 민감하게 군집 내 세포를 계측하고 마이크로플레이트 형식에서 증식을 측정할 수 있는 방법을 제공합니다.•자세히 알아보기
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카탈로그 번호세포 유형수량
C35011Direct Cell10 Microplates
C35013Direct Red Cell10 Microplates
C35012Direct Cell100 Microplates
C35007NF Cell200 Assays
C7026For cells in culture1000 Assays
C35006NF Cell1000 Assays
C7027Cell Lysis Buffer50 mL
카탈로그 번호 C35011
제품 가격(KRW)
661,000
온라인 행사
Ends: 31-Dec-2025
734,000
할인액 73,000 (10%)
Each
카트에 추가하기
세포 유형:
Direct Cell
수량:
10 Microplates
제품 가격(KRW)
661,000
온라인 행사
Ends: 31-Dec-2025
734,000
할인액 73,000 (10%)
Each
카트에 추가하기
CyQUANT™ NF Cell Proliferation Assay Kit는 빠르고 민감하게 군집 내 세포를 계측하고 마이크로플레이트 형식에서 증식을 측정할 수 있는 방법을 제공합니다.
• MTT 또는 AlamarBlue™ assay 보다 민감
• 선형 검출 범위 100 ∼ 20,000 cell/well (96-well microplate)
• 1시간 내에 분석을 완료할 수 있습니다.

빠르고 간편한 세포 증식 측정 방법
CyQUANT™ NF Cell Proliferation assay는 세포 용해, 긴 배양, 방사능, 세포 염색 제거 등이 필요하지 않습니다. CyQUANT™ NF assay는 세포막 투과 시약과 함께 세포 투과성 DNA 결합 dye를 사용하여 기존 CyQUANT™ 세포 증식 분석과 달리 동결-해동 세포 용해 단계가 필요하지 않습니다. CyQUANT™ NF Cell Proliferation Assay Kit는 96-well 또는 384-well 마이크로플레이트 형식으로 사용할 수 있으며 다음 두 가지 구성으로 제공됩니다. 샘플 크기가 작을 때에는 200 assay kit (C35007), high-throughput 어플리케이션에는 1000 assay kit (C35006)를 사용하십시오.

AlamarBlue™는 TREK Diagnostic Systems의 등록 상표입니다.

본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 유형Direct Cell
배양 환경Suspension Cell Culture, Adherent Cell Culture
검출 방법Fluorescence
염료 유형Other Label(s) or Dye(s)
여기/방출508/527 nm
형식96-well plate, 1536-well plate, 384-well plate
인큐베이션 시간60 min
수량10 Microplates
배송 조건Room Temperature
색상Green
EmissionVisible
용도(애플리케이션)Proliferation Assay
용도 (장비)Microplate Reader, Spectrophotometer, Microscope, HTS Reader
제품라인CyQUANT
제품 유형Cell Proliferation Assay
Unit SizeEach
구성 및 보관
  • 0.5 mL CyQUANT™ Direct nucleic acid stain (component A)
  • 2.5 ml CyQUANT™ Direct background suppressor I (component B)
  • Store both at 2°C to 25°C. Desiccate and protect from light.

자주 묻는 질문(FAQ)

MTT cell proliferation plate assays require cellular metabolism to modify the reagent and thus, only live cells will be counted. Is this true for CyQUANT Direct Cell Proliferation Assay, too?

This is true for MTT (as well as XTT, AlamarBlue Cell Viability Reagent, and PrestoBlue Cell Viability Reagent). CyQUANT Direct will also only count live cells, but for a different reason. The dye in it is a green-fluorescent nucleic acid stain, which will bind to DNA in all cells, live or dead, without the need for cellular metabolism. However, there is a non-cell-permeable quenching reagent in the kit which will both quench extracellular fluorescence (and thus this is a no-wash assay) AND will quench the fluorescence in dead cells (but not live cells).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

MTT cell proliferation assays require cellular metabolism to turn over the reagent, and thus only live cells will be counted. Is this true for CyQUANT Direct as well?

This is true for MTT (as well as XTT, AlamarBlue Cell Viability Reagent, and PrestoBlue Cell Viability Reagent). CyQUANT Direct will also only count live cells, but for a different reason. The dye is a green-fluorescent nucleic acid stain, which will bind to DNA in all cells, live or dead, without the need for cellular metabolism. However, there is a cell impermeant quenching reagent in the kit which will quench both extracellular fluorescence (and thus this is a no-wash assay) and the fluorescence in dead cells (but not live cells).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What volumes should I use to make a 10X stock solution for the CyQUANT Direct Cell Proliferation Assay (Cat. No. C35011, C35012)?

To make a 10X stock solution for the CyQUANT Direct Cell Proliferation Assay (Cat. No. C35011, C35012), we suggest using the following volumes:

1.1 mL         PBS
25 µL         CyQUANT Direct nucleic acid stain (Component A)
125 µL         CyQUANT Direct background suppressor I (Component B)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Are the components of the CyQUANT Direct Red Cell Proliferation Assay and CyQuant Direct Cell Proliferation Assay kits interchangeable?

No. The components of the CyQUANT Direct Red Cell Proliferation Assay and CyQuant Direct Cell Proliferation Assay kits are not interchangeable.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between the CyQUANT Direct Red Cell Proliferation Assay (Cat. Nos. C35013, C35014) and the green fluorescent CyQuant Direct Cell Proliferation Assay (Cat Nos. C35011, C35012)?

The two kits utilize different dyes and background suppressors, but they function in the same way and provide similar results as can be seen from Figures 1 and 2 in the CyQUANT Direct Red Cell Proliferation Assay product sheet (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018644_CyQUANT_direct_red_assay_PI.pdf). Dyes from both kits are nucleic acid stains and the background suppressors are cell impermeant reagents that quench extracellular fluorescence. They are usable within the same range (100 to 20,000 cells/well in a 96-well format) and in the same workflow.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all CyQUANT™ Cell Proliferation Assays FAQs

인용 및 참조 문헌 (7)

인용 및 참조 문헌
Abstract
Application of a high-content multiparameter cytotoxicity assay to prioritize compounds based on toxicity potential in humans.
Authors:Abraham VC, Towne DL, Waring JF, Warrior U, Burns DJ,
Journal:J Biomol Screen
PubMed ID:18566484
'Prioritization of compounds based on human hepatotoxicity potential is currently a key unmet need in drug discovery, as it can become a major problem for several lead compounds in later stages of the drug discovery pipeline. The authors report the validation and implementation of a high-content multiparametric cytotoxicity assay based ... More
Activator of G protein signaling 3 promotes epithelial cell proliferation in PKD.
Authors:Nadella R, Blumer JB, Jia G, Kwon M, Akbulut T, Qian F, Sedlic F, Wakatsuki T, Sweeney WE, Wilson PD, Lanier SM, Park F,
Journal:J Am Soc Nephrol
PubMed ID:20488951
'The activation of heterotrimeric G protein signaling is a key feature in the pathophysiology of polycystic kidney diseases (PKD). In this study, we report abnormal overexpression of activator of G protein signaling 3 (AGS3), a receptor-independent regulator of heterotrimeric G proteins, in rodents and humans with both autosomal recessive and ... More
The matricellular protein cysteine-rich protein 61 (CCN1/Cyr61) enhances physiological adaptation of retinal vessels and reduces pathological neovascularization associated with ischemic retinopathy.
Authors:Hasan A, Pokeza N, Shaw L, Lee HS, Lazzaro D, Chintala H, Rosenbaum D, Grant MB, Chaqour B,
Journal:J Biol Chem
PubMed ID:21212276
'Retinal vascular damages are the cardinal hallmarks of retinopathy of prematurity (ROP), a leading cause of vision impairment and blindness in childhood. Both angiogenesis and vasculogenesis are disrupted in the hyperoxia-induced vaso-obliteration phase, and recapitulated, although aberrantly, in the subsequent ischemia-induced neovessel formation phase of ROP. Yet, whereas the histopathological ... More
Selective blockade of cytoskeletal actin remodeling reduces experimental choroidal neovascularization.
Authors:Caballero S, Yang R, Grant MB, Chaqour B,
Journal:Invest Ophthalmol Vis Sci
PubMed ID:21178140
'The efficacy of the peptide Ac-EEED on reducing cell adhesion and proliferation in vitro and choroidal neovascularization (CNV) in vivo was examined. The peptide chimera containing the Ac-EEED sequence was chemically linked to the N terminus of the XMTM delivery peptide from the E(rns) viral surface protein. Ac-EEED or scrambled ... More
Loss of activator of G-protein signaling 3 impairs renal tubular regeneration following acute kidney injury in rodents.
Authors:Regner KR, Nozu K, Lanier SM, Blumer JB, Avner ED, Sweeney WE, Park F,
Journal:FASEB J
PubMed ID:21343176
The intracellular mechanisms underlying renal tubular epithelial cell proliferation and tubular repair following ischemia-reperfusion injury (IRI) remain poorly understood. In this report, we demonstrate that activator of G-protein signaling 3 (AGS3), an unconventional receptor-independent regulator of heterotrimeric G-protein function, influences renal tubular regeneration following IRI. In rat kidneys exposed to ... More
View all CyQUANT™ Cell Proliferation Assays citations