NuPAGE™ 3 a 8 %, Tris-Acetato, 1,0–1,5 mm, geles de proteínas Mini
NuPAGE™ 3 a 8 %, Tris-Acetato, 1,0–1,5 mm, geles de proteínas Mini
Citas y referencias (7)
Invitrogen™

NuPAGE™ 3 a 8 %, Tris-Acetato, 1,0–1,5 mm, geles de proteínas Mini

Los geles de proteína de Tris-acetato Invitrogen NuPAGE proporcionan una separación excelente de proteínas de alto peso molecular. Son gelesMás información
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Número de catálogoPocillosGel ThicknessCantidad
EA0375BOX10 pocillo1,0 mm10 geles/caja
EA0375PK210 pocillo1,0 mm2 geles/caja
EA03752BOX12 pocillo1,0 mm10 geles/caja
EA03752PK212 pocillo1,0 mm2 geles/caja
EA03755BOX15 pocillo1,0 mm10 geles/caja
EA0378BOX10 pocillo1,5 mm10 geles/caja
EA03785BOX15 pocillo1,5 mm10 geles/caja
Número de catálogo EA0375BOX
Precio (USD)
-
Pocillos:
10 pocillo
Gel Thickness:
1,0 mm
Cantidad:
10 geles/caja
Los geles de proteína de Tris-acetato Invitrogen NuPAGE proporcionan una separación excelente de proteínas de alto peso molecular. Son geles de poliacrilamida de alto rendimiento que simulan las condiciones de desnaturalización o nativas del sistema tradicional laemmli. La fórmula de tampón única mantiene un pH de funcionamiento bajo durante la electroforesis, lo que resulta en una resolución superior de proteínas de alto peso molecular en comparación con los geles de Tris-glicina SDS-PAGE tradicionales.

Características de los geles de Tris-acetato NuPAGE:
• Alta resolución: los geles ofrecen una separación óptima de proteínas de alto peso molecular
• Mejor integridad de las proteínas: se ha optimizado el proceso de preparación de muestras para ayudar a preservar las proteínas
• Vida útil más larga: los geles se pueden almacenar durante al menos ocho meses

Obtenga más información sobre nuestros geles de Tris-acetato NuPAGE ›
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Elija el gel de Tris-acetato NuPAGE adecuado para la separación de proteínas
Obtenga una separación óptima de sus proteínas de alto peso molecular eligiendo la combinación correcta de gel y tampón de desplazamiento. Los geles de proteína de Tris-acetato NuPAGE vienen en una concentración de poliacrilamida del 7 % y un gradiente del 3–8 %. Los geles se suministran en dos tamaños: mini (8 cm x 8 cm) o medio (8,7 cm x 13,3 cm), y 1,0 mm (geles mini y medio) o 1,5 mm (solo formato minigel) de grosor. Los geles de Tris-acetato NuPAGE también se suministran con múltiples formatos de pocillo. Los minigeles se pueden ejecutar mediante nuestra minicelda XCell SureLock o depósito de minigel. Los geles medios se pueden ejecutar mediante nuestra celda media XCell4 SureLock o, convenientemente, con la celda Bio-Rad Criterion™ usando nuestros adaptadores.

Ejecute sus proteínas en forma nativa o desnaturalizada
Los geles de proteína de Tris-acetato NuPAGE no contienen SDS y se pueden utilizar para separar proteínas en forma nativa o desnaturalizada. Para la desnaturalización de las proteínas, le recomendamos utilizar el tampón de muestra LDS NuPAGE y el tampón de desplazamiento de Tris-acetato SDS NuPAGE. Para proteínas nativas, le recomendamos utilizar el tampón de muestra nativo de Tris-glicina Novex y el tampón de desplazamiento nativo de Tris-glicina Novex.

Para la transferencia de proteínas a una membrana, le recomendamos utilizar el tampón de transferencia NuPAGE para la transferencia húmeda tradicional utilizando el módulo blot XCell II o el módulo Mini Blot. También se puede realizar una transferencia semiseca rápida con Invitrogen Power Blotter o una transferencia rápida en seco con el dispositivo de transferencia de gel iBlot 2.

Enlaces relacionados
Descripción general de la electroforesis de proteínas 1D
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Gel Thickness1,0 mm
Longitud (métrico)13 cm
Modo de separaciónPeso molecular
Línea de productosNuPAGE
Cantidad10 geles/caja
Aplicaciones recomendadasDesnaturalización, nativa
Volumen de carga de muestrasHasta 25 µl
Duración de almacenamiento8 meses
Condiciones de envíoHielo húmedo
Requisitos de almacenamientoAlmacenar entre 2 °C y 8 °C. No la congele.
Anchura (métrico)8 cm
Para utilizar con (equipo)Depósito de minigel, Minicelda XCell SureLock
Porcentaje del gelDel 3 al 8%
Tamaño de gelMini
Tipo de gelTris-acetato
Intervalo de separaciónDe 36 a 500 kDa
Tipo de separaciónDesnaturalización, nativa
Pocillos10 pocillo
Unit SizeEach
Contenido y almacenamiento
Una caja contiene 10 geles. Almacenar en el refrigerador (2–8° C). No la congele. La vida útil es de 6 meses.

Preguntas frecuentes

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What is the advantage of NuPAGE Gels over regular Tris-Glycine gels?

The neutral operating pH of the NuPAGE Gels and buffers provides following advantages over the Laemmli system:
-Longer shelf life of 8-12 months due to improved gel stability
-Improved protein stability during electrophoresis at neutral pH resulting in sharper band resolution and accurate results (Moos et al, 1998)
-Complete reduction of disulfides under mild heating conditions (70 degrees C for 10 min) and absence of cleavage of asp-pro bonds using the NuPAGE LDS Sample buffer (pH > 7.0 at 70 degrees C)
-Reduced state of the proteins maintained during electrophoresis and blotting of the proteins by the NuPAGE Antioxidant
Please refer to the following paper: Moos M Jr, Nguyen NY, Liu TY (1988) Reproducible High Yield Sequencing of Proteins Electrophoretically Separated and Transferred to an Inert Support. J Biol Chem 263:6005-6008.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What does it mean when bands appear to be getting narrower (or "funneling") as they progress down a protein gel?

There may be too much beta-mercaptoethanol (BME), sample buffer salts, or dithiothreitol (DTT) in your samples. If the proteins are over-reduced, they can be negatively charged and actually repel each other across the lanes causing the bands to get narrower as they progress down the gel.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all NuPAGE™ 3 a 8 %, Tris-Acetato, 1,0–1,5 mm, geles de proteínas Mini FAQs

Citations & References (7)

Citations & References
Abstract
Cloning of a novel phosphatidylinositol kinase-related kinase: characterization of the human SMG-1 RNA surveillance protein.
Authors: Denning G; Jamieson L; Maquat L E; Thompson E A; Fields A P;
Journal:J Biol Chem
PubMed ID:11331269
'We have cloned and characterized a new member of the phosphatidylinositol kinase (PIK)-related kinase family. This gene, which we term human SMG-1 (hSMG-1), is orthologous to Caenorhabditis elegans SMG-1, a protein that functions in nonsense-mediated mRNA decay (NMD). cDNA sequencing revealed that hSMG-1 encodes a protein of 3031 amino acids ... More
Multimerization of the ligand binding domains of cyclic nucleotide-gated channels.
Authors: Matulef Kimberly; Zagotta William;
Journal:Neuron
PubMed ID:12367509
Cyclic nucleotide-gated (CNG) channels comprise four subunits and are activated by the direct binding of cyclic nucleotide to an intracellular domain on each subunit. This ligand binding domain is thought to contain a beta roll followed by two alpha helices, designated the B and C helices. To examine the quaternary ... More
Dismantling of cadherin-mediated cell-cell contacts modulates smooth muscle cell proliferation.
Authors:Uglow EB, Slater S, Sala-Newby GB, Aguilera-Garcia CM, Angelini GD, Newby AC, George SJ,
Journal:Circ Res
PubMed ID:12775583
Proliferation of vascular smooth muscle cells (VSMCs) contributes to intimal thickening during atherosclerosis and restenosis. The cadherins are transmembrane proteins, which form cell-cell contacts and may regulate VSMC proliferation. In this study, N-cadherin protein concentration was significantly reduced by stimulation of proliferation with fetal calf serum (FCS) and platelet-derived growth ... More
The cytoplasmic tail of L-selectin interacts with members of the Ezrin-Radixin-Moesin (ERM) family of proteins: cell activation-dependent binding of Moesin but not Ezrin.
Authors:Ivetic A, Deka J, Ridley A, Ager A,
Journal:J Biol Chem
PubMed ID:11706008
L-selectin regulates the recruitment of naive lymphocytes from the bloodstream to secondary lymphoid organs, mediating their initial capture and subsequent rolling along high endothelial cell surface-expressed ligands in peripheral lymph nodes. In vivo, distribution of L-selectin and cell surface levels determine the tethering efficiency and rolling velocity of leukocytes, respectively. ... More
The 'involution' of mannose-binding lectin.
Authors:Seyfarth J, Garred P, Madsen HO,
Journal:Hum Mol Genet
PubMed ID:16115813
Mannose-binding lectin (MBL) acts as a serum opsonin in innate immune defense and induces complement activation by the lectin pathway. In humans, low levels of functional serum MBL are caused by the dominant action of three single nucleotide substitutions in exon 1 that disrupt the glycine-rich backbone structure of the ... More
View all NuPAGE™ 3 a 8 %, Tris-Acetato, 1,0–1,5 mm, geles de proteínas Mini citations