Do I need to add water or buffer to the Displacement Dam when I run one or two gels in the XCell4 SureLock Midi-Cell?
The XCell4 Displacement Dam is used when fewer than 3 gels are run in the XCell4 SureLock Midi-Cell. The Displacement Dam is placed in the lower buffer chamber instead of a second buffer core. Do not add any buffer or water to the Displacement Dam. However, if water or buffer is accidentally added to the Displacement Dam, it does not affect the electrophoresis run.
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What are the maximum load volumes for the NuPAGE Midi Gels?
Recommended maximum load volumes:
12 + 2-well gel: 45 µL in sample well, 15 µL in marker well
20-well gel: 25 µL
26-well gel: 15 µL
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What do I need to run the NuPAGE Invitrogen Midi Gels in the Bio-Rad Criterion Cell?
You will need a Midi Gel Adapter. The Midi Gel Adapter is a self-adhesive, upper buffer chamber used when running the NuPAGE Invitrogen Midi-Gel in a Bio-Rad Criterion Cell. The Midi Gel Adapter may be filled with up to 60 mL of buffer. The Midi Gel Adapters are available packaged with the Midi-Gels (Invitrogen and NuPAGE Invitrogen Midi Gels with Cat. Nos. that end in "A") and also on their own in packages of 10 (Cat. No. WA0999). Running the NuPAGE Midi-Gels in the XCell4 SureLock Gel Apparatus does not require the use of the Midi Gel Adapter.
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Are the NuPAGE Midi Gels compatible with multichannel pipettes?
Multichannel pipette compatibility: 12+2-well (8- and 12-channel pipette-compatible) and 26-well (8- and 12-channel pipette-compatible).
The 12-channel pipettes work with the 26-well gels. You will need to alternate lanes (e.g., aligns with every two lanes). There will be two extra lanes (for MW markers). The 12-channel pipettes work with the 12+2 well gels as well.
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How much of the pAd/CMV/V5-DEST or pAd/PL-DESTpAD destination vector do I need to add in the LR reaction?
One should use 2 µl = 300 ng pAd-DEST vector. Please Note that Version D of the pAd/CMV/V5-DEST and pAd/PL-DEST Gateway
Vectors product manual (manual number 25-0544) had an error on page 9 regarding this protocol. The manual instructed users to add 1 µl, but this has since been corrected to reflect the correct protocol of addition of 2 µl.
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