AMAS (N-α-maleimidoacet-oxysuccinimide ester)

Zitierungen und Referenzen (4)

Thermo Scientific™

AMAS (N-α-maleimidoacet-oxysuccinimide ester)

Katalognummer	Menge
22295	50 mg

Katalognummer 22295

Preis (EUR)

191,00

Menge:

50 mg

Großbestellung oder individuelle Größe anfordern

Preis (EUR)

191,00

Thermo Scientific Pierce AMAS ist ein Amin-zu-Sulfhydryl-Crosslinker, der NHS-Ester und Maleimid-reaktive Gruppen an gegenüberliegenden Enden eines kurzen Spacerarms (4,4 Angström) enthält.

Merkmale von AMAS:

• Reaktive Gruppen: NHS-Ester und Maleimid
• Reaktiv gegen: Amino- und Sulfhydrylgruppen
• Kürzeste Serie aliphatischer Spacer, die AMAS, BMPS, GMBS und EMCS enthält
• NHS-Ester-Endpaare mit primären Aminen bei pH 7 bis -9 enthält und stabile Amidbindungen bildet
• Maleimid reagiert mit -SH-Gruppen bei einem pH-Wert von 6,5 bis 7,5, Bildung stabiler Thioether-Verbindungen
• Nicht spaltbar
• Wasserunlöslich (zuerst in DMF oder DMSO auflösen)
• Sehr kurzer aliphatischer Spacer hat ein geringes Potenzial, eine Immunantwort zu eluieren

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

ZellpermeabilitätJa

BeschreibungAMAS

FormPulver

MarkierungsmethodeChemische Markierung

Molekulargewicht252,18

PEGyliertNein

ProduktliniePierce

Menge50 mg

Reaktiver TeilMaleimid, NHS-Ester

VersandbedingungUmgebungstemperatur

LöslichkeitDMF, DMSO

Länge des Spacer-Arms4,4 Å

WasserlöslichNein

Chemische ReaktivitätAmin-Sulfhydryl

CleavableNein

Crosslinker-TypHeterobifunktional

FormatStandard

ProdukttypCrosslinker

AbstandshalterKurz (<10 Å)

Unit SizeEach

Inhalt und Lagerung

Nach Erhalt getrocknet bei 4 °C lagern.

Häufig gestellte Fragen (FAQ)

Can you provide the shelf-life for AMAS (N-α-maleimidoacet-oxysuccinimide ester)?

AMAS (N-α-maleimidoacet-oxysuccinimide ester is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Zitierungen und Referenzen (4)

Zitierungen und Referenzen

Abstract

Dual binding mode of the nascent polypeptide-associated complex reveals a novel universal adapter site on the ribosome.

Authors:Pech M, Spreter T, Beckmann R, Beatrix B

Journal:J Biol Chem

PubMed ID:20410297

'Nascent polypeptide-associated complex (NAC) was identified in eukaryotes as the first cytosolic factor that contacts the nascent polypeptide chain emerging from the ribosome. NAC is present as a homodimer in archaea and as a highly conserved heterodimer in eukaryotes. Mutations in NAC cause severe embryonically lethal phenotypes in mice, Drosophila ... More

Analysis of RovA, a transcriptional regulator of Yersinia pseudotuberculosis virulence that acts through antirepression and direct transcriptional activation.

Authors:Tran HJ, Heroven AK, Winkler L, Spreter T, Beatrix B, Dersch P

Journal:J Biol Chem

PubMed ID:16257976

'The transcription factor RovA of Yersinia pseudotuberculosis and analogous proteins in other Enterobacteriaceae activate the expression of virulence genes that play a crucial role in stress adaptation and pathogenesis. In this study, we demonstrate that the RovA protein forms dimers independent of DNA binding, stimulates RNA polymerase, most likely via ... More

A central role for the T1 domain in voltage-gated potassium channel formation and function.

Authors:Strang C, Cushman SJ, DeRubeis D, Peterson D, Pfaffinger PJ

Journal:J Biol Chem

PubMed ID:11312262

To interpret the recent atomic structures of the Kv (voltage-dependent potassium) channel T1 domain in a functional context, we must understand both how the T1 domain is integrated into the full-length functional channel protein and what functional roles the T1 domain governs. The T1 domain clearly plays a role in ... More

Spatial and dynamic interactions between phospholamban and the canine cardiac Ca2+ pump revealed with use of heterobifunctional cross-linking agents.

Authors:Chen Z, Stokes DL, Rice WJ, Jones LR

Journal:J Biol Chem

PubMed ID:12972413

Heterobifunctional thiol to amine cross-linking agents were used to gain new insights on the dynamics and conformational factors governing the interaction between the cardiac Ca2+ pump (SERCA2a) and phospholamban (PLB). PLB is a small protein inhibitor of SERCA2a that reduces enzyme affinity for Ca2+ and thereby regulates cardiac contractility. We ... More