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Invitrogen

Phospho-SMAD2 (Ser255) Recombinant Rabbit Monoclonal Antibody (2A12)

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Phospho-SMAD2 (Ser255) Recombinant Rabbit Monoclonal Antibody (2A12)

Product Details

MA5-49772

Applications
Tested Dilution
Publications

Immunocytochemistry (ICC/IF)

1:20-1:200
-

ELISA (ELISA)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

2A12

Immunogen

A synthesized peptide derived from human Phospho-SMAD2 (S255)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.2 mg/mL

Amount

120 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3092647

Target Information

SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin. In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: hMAD-2; JV18-1; MAD; MAD homolog 2; Mad protein homolog; Mad-related protein 2; MGC22139; MGC34440; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; pSMAD2; Sma- and Mad-related protein 2; SMAD; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2; Smad2Deltaexon3; unnamed protein product

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Gene Aliases: CHTD8; hMAD-2; hSMAD2; JV18; JV18-1; LDS6; MADH2; MADR2; SMAD2

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UniProt ID: (Human) Q15796

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Entrez Gene ID: (Human) 4087

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding transforming growth factor beta receptor binding protein binding phosphatase binding ubiquitin protein ligase binding type I transforming growth factor beta receptor binding identical protein binding SMAD binding metal ion binding tau protein binding RNA polymerase II sequence-specific DNA binding transcription factor binding co-SMAD binding I-SMAD binding R-SMAD binding disordered domain specific binding DNA-binding transcription factor binding
Process(es)
ureteric bud development in utero embryonic development endoderm formation mesoderm formation regionalization determination of left/right asymmetry in lateral mesoderm aortic valve morphogenesis pulmonary valve morphogenesis endocardial cushion morphogenesis transcription, DNA-templated regulation of transcription, DNA-templated transforming growth factor beta receptor signaling pathway zygotic specification of dorsal/ventral axis gastrulation pattern specification process endoderm development mesoderm development heart development cell proliferation anatomical structure morphogenesis response to glucose post-embryonic development embryonic pattern specification anterior/posterior pattern specification positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial to mesenchymal transition regulation of transforming growth factor beta receptor signaling pathway insulin secretion cell differentiation negative regulation of ossification lung development positive regulation of BMP signaling pathway pancreas development primary miRNA processing activin receptor signaling pathway organ growth intracellular signal transduction nodal signaling pathway cell fate commitment negative regulation of cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter paraxial mesoderm morphogenesis developmental growth embryonic foregut morphogenesis embryonic cranial skeleton morphogenesis regulation of multicellular organismal process pericardium development SMAD protein signal transduction mesenchyme development trophoblast cell migration secondary palate development response to cholesterol response to transforming growth factor beta odontoblast differentiation
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